转化细胞

犬2型腺病毒E1转化细胞系的特性研究

The studies on CAV-2 sy strain E1 transformed cell line

同时，转化细胞系DNA、RNA和蛋白质出现最大合成速度的时间均相应地延迟。

Meanwhile , the appearance of the maximum synthesis rate for DNA , RNA and protein in transformed cell line exhibited was postponed compared with control cell line .

显微分光光度计对水体有机污染物诱导的转化细胞DNA含量的分析

Analysis on DNA content of trasformation cells induced by extracts of water using microspectrophotometer

甲基硝基亚硝基胍诱发转化细胞环核苷酸和DNA碱基含量变化的探讨

Studies on the Changes of Cycle Nucleotide and Base Contents in Cells Transformed by MNNG

人体胃癌DNA转化细胞诱发裸鼠肿瘤的研究

Study on the tumours induced in nude mouse by malignant transformants of human gastric carcinoma DNA

多胺生物合成的抑制对转化细胞c－Ha－ras癌基因表达的调控

The Regulation of the Inhibition of Polyamine Biosynthesis to the Expression of c-Ha-ras Oncogene in Transformed Cells

检测了Ras癌基因对转化细胞的生长和分化特性的作用。

The growth properties and differentiation of Ras transfectants were examined .

紫外线照射或H2O2处理转化细胞使DNA损伤，诱导细胞内源性P53的表达。

Transformed cells were treated with ultraviolet and H 2O 2 to make DNA damage and induce endogenous P53 expression .

绿色荧光蛋白GFP在转化细胞和肿瘤细胞中的表达

The Expression of GFP Gene in Transformed and Tumor Cells

为了进一步研究转化细胞的DNA合成情况，我们又进行了3H－TdR掺入研究。

Further study on the DNA synthesis of such transformed cells , 3 H-TdR incorporation method was adopted .

反义核酸抑制靶基因表达对EB病毒转化细胞的生长控制研究

Inhibiting Target Gene Expression and Controlling Growth of Epstein-Barr Virus Transformed Cells by Antisense RNA Transcripts

转化细胞可被低浓度conA凝集，并在软琼脂内生长。

The transformed cells could be agglutinated by low concentration of Con A and grow in semisolid agar .

以苜蓿转化细胞系和对照细胞系为材料，利用同位素标志方法研究了3种大分子物质&DNA、RNA和蛋白质的合成动态。

The synthesis of three kinds of macromolecular substances-DNA , RNA and protein in transformed and control cell line of Alfalfa was studied using isotope-tracing methods .

表达短lef-1dsRNA的转化细胞对家蚕核型多角体病毒的抗性核盘菌中dsRNA种类及其与致病力的关系

Resistance to BmNPV of Transformation Cells Expressing Short lef-1 dsRNA

细小病毒是一组无囊膜，线状，裂解性的DNA病毒，它们对转化细胞具有杀伤效应而对相应的非转化细胞则无害，是基因治疗的候选载体。

Autonomous parvoviruses are nonenveloped , linear-stranded , lytic DNA viruses which are cytotoxic to neoplastic cells while innocuous to their non-transformed counterparts , thus become a candidate of choice as vehicles in gene therapy .

GUS活性检测和胭脂碱分析表明，外源基因在转化细胞内得到稳定表达。

Enzyme activity of GUS and NOS assay demonstrated that foreign genes expressed in the transformed plants and calli .

反义PARP基因真核表达重组体的构建及其HeLa转化细胞系的建立

Construction of antisense PARP cDNA recombinants and establishment of the HeLa transformant after antisense plasmid introducing

db-cAMP对转化细胞增殖抑制及其对CaM水平和PKⅡ活性的影响

The effects of db-cAMP on cam level , pk ⅱ activity and inhibition of transformed cells proliferation

非稳定性畸变在多次传代的转化细胞中仍然存在，说明微量~3HTdR可能残留于DNA中，持续对细胞造成损伤。

The fact that unstable aberrations was Still in sight in the malignant transformation cells suggested that there have been a bit of 3 ~ HTdR left in these cells which kept damaging DNA of the cells .

bcr-abl转化细胞中的信号转导途径

Signal Transduction Pathways in bcr-abl Transformed Cells

通过细胞形态及生物学特性观察、Southern杂交实验、软琼脂集落形成实验和P21蛋白表达量的检测等实验对转化细胞进行了研究。

The transfected cells were selected by drug selection . The morphology change , Southern hybridization , frequency of soft agar colonies and the detection of P21 amount were investigated .

对转化细胞系采用Southernblot、NorthernBlot及westernblot杂交分析，证实有稳定的外源基因的整合及表达，而内源性TGFα的表达及合成均

The integration of retrovirus vector and expression of exogenous gene in the transformant PC-7 cells were confirmed by Southern blot , Northern blot and Western blot hybridizations . The gene expression and protein

PARP酶抑制剂诱导转化细胞逆转及外源ras癌基因丢失的研究

Studies on Reversion of a Transformant Line and Loss of Exogenous ras Oncogene after Treatment with an Inhibitor of PARP Enzyme

其中，VP3蛋白质是鸡贫血病毒的致病因子，能选择性的诱导肿瘤细胞和转化细胞发生凋亡，但对人和鼠的正常二倍体细胞无影响，称为凋亡素（apoptin）。

Apoptin ( VP ,) can selectively induce apoptosis in human transformed and malignant cells but not in normal diploid cells .

组蛋白乙酰化修饰对IEC-6恶性转化细胞细胞周期和p53、p21~（WAF1）基因表达的调控

Regulation of histone acetylation on cell cycle and p53 , p21 ~ ( WAF1 ) genes expression in malignant transformation of IEC-6

在一些癌基因的转化细胞系和几乎所有的动物肿瘤中均有ODC基因产物的异常升高。

Elevated levels of ODC gene products are consistantly detected in some oncogene transformed cell lines and virtually all animal tumors .

根据转化细胞合成的冠瘿碱（opine）的类型不同，Ri质粒分为3种类型：甘露碱型（mannopinetype）、黄瓜碱型（cucumopinetype）和农杆碱型（agropinetype）。

There are three types of Ri plasmid , mannopine type , cucumopine type and agropine type , according to the type of opine synthesized in transgenic cell .

转化细胞和正常细胞中核仁蛋白B23的含量差异

Difference in the Level of Nucleolar Protein B 28 between Transformed and Normal Mammalian Cells

结论IMCC保留了MCC的血清依赖性生长特性，其增殖速度快于MCC，不属于恶性转化细胞。

Conclusion IMCC grows faster than MCC and retains the serum-dependent growth character of MCC , which not belong to malignant transformed cells .

这些带有GFP标记的转化细胞系为柑橘的有关基础研究提供了良好的试材，目前正用于柑橘体细胞杂交和不同倍性愈伤组织的社会化控制现象与机理等研究。

These transgenic cell lines are valuable materials in citrus basic research and currently being used in studies on citrus somatic fusion and social control mechanism between calluses of different ploidy levels .