茎尖
- 名tip
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柑桔茎尖培养的研究I.茎尖培养与增殖
Studies on Shoot Tip Culture of Citrus I.Shoot Tip Culture and Its Propagation
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体细胞胚经过球形胚、心形胚、鱼雷形胚和子叶胚而发育成再生植株,子叶期体细胞胚可观察到明显的V型维管束、茎尖分生组织以及根尖分生组织。
Plantlet regenerations were developed from global embryo , heart-shaped embryo , torpedo-shaped embryo and cotyledon embryo , in which the V-shaped vessel vascular bundles and shoot tip meristem and root tip meristem were observed .
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水蜜桃茎尖基因组DNA不同提取方法研究
Study on the Different Extraction Method of Genomic DNA of Juicy Peach Stem-tip
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外源DNA导入烤烟后变异株D1代茎尖染色体核型分析
Chromosome Karyotype Analysis Of Stem Apex from Transformed with Foreign DNA mutant D_1 Generation Tobacco
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对茎尖进行了转化,只获得抗性植株,但未检测到PCR阳性植株。
The shoot meristems were transformed . But no plants were positive , according to PCR results . 6 .
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1以东方百合品鳞片为外植体,采用MS培养基和不同外源激素配比,进行组织培养,研究其茎尖分生组织分化。
Meristem culture of Oriental Hybrid Lily through its scales was studied in MS medium with different external hormone treatments .
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由茎尖途径培养获得119个F2无性系株系,其再生植株在苗期株系内生长整齐一致;
The regeneration plants 119 materials of F2 clonal population from stem tipes growed uniformly in lines in seedling stage ;
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农杆菌介导的棉花茎尖遗传转化及转betA植株的产生
Agrobacterium-mediated Transformation of Shoot Apex of Cotton and Production of Transgenic Plants Carrying BetA Gene
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结果表明,不同生育期白菜茎尖DNA甲基化水平不同,营养生长期高于生殖生长期。
The results showed that the DNA methylation level of shoot apices at different stages was different , That at vegetative stage was higher than reproductive stage .
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不同采摘期甘薯茎尖中的硝酸盐亚硝酸盐和VC含量分析
Analysis of the Contents of Nitrate , Nitrite and VC in the Stem-apex of Sweet Potatoes in Different Picking Stages
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以茎尖为外植体,采用MS培养基为基本培养基,适当调整激素浓度,进行白网纹草丛生芽诱导技术及外植体消毒技术研究。
Stem-point is used as explant . And medium MS is used as basic medium . Concentration of internal secretion is adjusted properly .
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6-BA对大豆茎尖诱导再生植株的研究
Study on 6-BA to the regeneration of tip shoot of soybean
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以叶用莴苣微茎尖为试材,在含有BA和NAA的MS液体培养基上旋转培养,诱导出大量愈伤组织;
Primordium-like tissue was established from the apical meristem under rotation culture on MS medium with BA and NAA .
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45℃2h以内热激处理可促进菜薹茎尖的增殖。
Heat shock with shoot-tips at 45 ℃ for 2h could stimulate the proliferation of shoots .
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6-BA诱导大豆子叶节和茎尖出芽的研究
Studies on Shoots Induced by 6-BA from Cotyledonary Nodes and Embryonic Tips of Soybean
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SOD在茎尖分布最多,而POD则以活动根分布为最多,差异均达极显著水平,供试的5个品种,其SOD活性差异显著,表明了它们对水分胁迫反应的不一致性。
There were significant differences in SOD activities among the 5 tested cultivars , which indicated that loquat cultivars had different responses to water defficiency stress .
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杨和苹果离体茎尖培养和愈伤组织分化与内源IAA、ABA的关系
Relationship Between the Formation of Shoot Apexes and Calli Differentiation of Poplar and Apple in vitro and Endogenous IAA and ABA
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用不同浓度的6BA对茎尖进行不同时间的处理,不同的品种对6BA的效果不一致。
To treat the apexes with 6-BA with different concentration for different time , we found that the effect was distinct with different varieties .
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春化处理后,两处理植株茎尖组织DNA甲基化水平都较对照下降;但GA含量明显上升,为对照的2~3倍;
After vernalization , DNA methylation level in shoot apices under both treatment condition was decreased comparing with control plants , but GA content was increased to 2-3 times of control 's.
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以河北省两个名优地方品种安国大蒜和永年大蒜为试材,用Ms,B5作基本培养基,研究了大蒜茎尖繁殖脱毒快繁技术。
The virus-eliminating technology with shoot-tip culture and rapid propagation of two local famous elite garlic varieties in Hebei province were studied .
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转基因植株ZR的含量在茎尖和根系中的变化不一致。
Under different IBA concentrations , The content of ZR in shoot tip and roots of the transgenic plants are inconsistent . 3 .
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茎尖途径形成的再生植株R1代变异系数分析发现:在一次有效枝起点上表现出的差异最大,变异频率为44.54%;
The result of analysis about CV ( coefficient of variation ) in R_1 generation suggested that variation in zero point for 1st branches was the highest , and it 's frequency of variation was 44.54 % ;
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21d后调查,茎尖存活率达84.6%。
The survival rate of the shoot tips reached 84.6 % , after 21d regrowth .
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方法剥取不大于1mm的姜茎尖生长点为外植体,以MS为基本培养基,附加不同种类和浓度的植物生长物质进行培养。
Methods The ginger stem apex ((?) < 1 mm ) was taken as explants and cultured on MS medium with different portions of plant growth substances .
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GUS染色结果显示,POS基因在根、叶、茎尖、节位和分蘖芽都有表达。
Staining results showed that POS mRNA was detected in vegetative organs , including root , leaf , shoot apex , upper node and tiller .
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诱导茎尖成苗的培养基为MS+6-BA0.5mg/L。繁殖脱毒苗所用的MS培养基为1/4大量元素+1/2微量元素,并用1/2食用白砂糖代替蔗糖,可使快繁成本降低48.7%。
The MS media for seedlings virus-free culture are 1 / 4 macro-elements + 1 / 2 micro-elements and using edible sugar instead of sucrose , so the cost of media could be decreased to 48.7 % .
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0.2%NaCl明显抑制试管苗的生长;但当培养基中NaCl浓度高达1.0%时,试管苗茎尖和上部叶片仍保持绿色。
While plantlets were obviously stunted at NaCl higher than 0.2 % , their shoot - tips and upper leaves remained green even when 1.0 % NaCl was included in the media .
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泡桐微茎尖培养脱除泡桐丛枝病原(MLO)的研究
Eradication of Paulownia Witches ' Broom Agent ( MLO ) from Infected Plantlet by Meristem Tip Culture
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甘薯茎尖和薯块的多酚含量与绿原酸及其异构体的含量、比例与其DPPH清除能力相关。
The DPPH-scavenging activities of vine tips and roots of sweetpotato were related positively to polyphenolic content and chlorogenic acid and its isomer content .
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初步建立了茎尖培养快速繁殖COLt苗木的技术程序,为实际应用开辟了新途径。
The technical process of rapid propagation with stem tip was primary established and explored a new pathway for practical application .