转醛醇酶

目的：研究小鼠间充质细胞（K9）中人绒毛膜促性腺激素（hCG）对转醛醇酶（TAL）活性的调节。

AIM : To study the regulation of human chorionic gonadotropin ( hCG ) on the activity of transaldolase ( TAL ) in K9 cell line .

Lachaise等以及本研究室已有的研究表明，在无脊椎动物螃蟹的蜕皮甾酮和小鼠睾丸间质细胞的睾酮生成过程中，转醛醇酶的表达和活性与之呈密切正相关。

Previous study of Lachaise 's lab and our lab had demonstrated that the expression and activity of Transaldolase was positively related to the production of ecdysterone in the invertebrate-crab and testosterone in the mouse Leydig cells .

以RTPCR方法检测转醛醇酶mRNA水平差异，以免疫印迹方法测定转醛醇酶蛋白表达水平差异；

By RT-PCR and Western blotting Transaldolase expression in mRNA level and protein level were determined .

Lachaise等研究表明在螃蟹蜕皮腺中，蜕皮甾酮的合成与转醛醇酶表达、活性呈密切正相关，环鸟苷酸介导的蛋白激酶促进转醛醇酶的磷酸化；

Previous research of Lachaise had demonstrated that in crab steroidogenic organ , ecdysterone synthesis was positively related to expression and enzymatic activity of Transaldolase .

龙眼转醛醇酶基因的克隆与原核表达

Cloning and Prokaryotic Expression of Transaldolase from Longan

转醛醇酶在瘢痕疙瘩组织中的表达

The Expression of Transaldolase in Keloid Tissues

结果显示转醛醇酶在生后7天小鼠睾丸中表达量平均比生后40天小鼠高47.8%；

Transaldolase expression in testes of 7-day-old mice were 47.8 % higher that in 40-day-old mice .

实验结果证实转醛醇酶活性与青春期小鼠睾丸间质细胞生成睾酮是密切相关的；

Results demonstrated that Transaldolase activity was closely correlated with testosterone synthesis in mice Leydig cells .

并且蜕皮甾酮合成的负调控因素&蜕皮抑制激素也能同时抑制着转醛醇酶的表达和活性。

Molt inhibiting hormone , the negative regulator of ecdysterone , can also inhibit the expression and activation of Transaldolase ;

转醛醇酶的表达和活性表现出明显的组织特异性、发育阶段相关性和生理病理状态差异性。

The expression and activation of Tranaldolase have tissue-specificity , correlating with the different develop phase and the difference between physiological and pathological condition .

结果：①转醛醇酶在生后1周小鼠睾丸中表达量平均比成年高47.8%（P<0.005）。

Results : ① Expression of transaldolase in testes of one week old mice was 47.8 % higher than that in adult mice ( P < 0.005 ) .

实验共分为两个部分进行：第一部分研究转醛醇酶在小鼠睾丸中有无表达，并利用蛋白质组学的思路和方法来分析转醛醇酶在小鼠睾丸中是否存在亚型；

The study was divided into two parts : the first part was to probe whether Transaldolase was expressed in mice testes and whether it has any isoforms .