SCNT

SCNT embryos often shows high levels of DNA methylation .

体细胞核移植重构胚常常表现为DNA甲基化水平过高。

The SCNT were performed to confirm the feasibility of optimized maturation media and activation protocol taking cow ear fibroblast , oviduct epithelial cells and cumulus cells as nuclear donors .

以体外培养的奶牛耳部成纤维细胞、输卵管上皮细胞及卵丘细胞为供体细胞，进行体细胞核移植证实了上述奶牛卵母细胞成熟培养液和激活方法的可行性。

Analysis of SC Networks Using SPICE & A Translation Program SCNT

利用SPICE分析开关电容网络的转换程序&SCNT

This study provides useful information for the SCNT and would benefit in promoting the efficiency .

本研究为转基因核移植技术提供了有价值的信息，有利于核移植效率的提高。

Effects of series nuclear transfer on buffalo SCNT were examined .

探讨了水牛体细胞连续核移植的效果。

This paper introduces the methods of zona-free SCNT and the single embryo culture system .

作者就无透明带核移植技术和单个胚胎培养系统作一介绍。

Thus , it is necessary to do more research on series nuclear transfer to improve the efficiency of buffalo SCNT .

说明连续核移植能否作为提高水牛体细胞核移植效率的一种手段尚需进一步研究。

Smooth cells or rough surface cells were used as donor in SCNT . Smooth surface cells led to a higher fusion rate .

用表面光滑细胞及表面粗糙细胞分别作为核移植供体，表面光滑的细胞可以提高供核细胞与受体卵母细胞的融合率。

Effect of BCB Selecting Oocytes Staining on the Developmental Competence of Bovine SCNT Embryos

亮甲酚蓝染色法筛选卵母细胞对牛体细胞克隆胚体外发育的影响

A Computer program SCNT is presented in this paper which can convert a two-phase SC network into its continuous-time model .

本文介绍一个可将双相开关电容网络转换为等效的连续时间电路的计算机程序SCNT。

But the subsequent studies showed that the most of the SCNT embryos in monkey had normal distribution of NuMA .

后来的研究显示，大部分猴子体细胞核移植胚胎NuMA分布基本正常。

The procedure of somatic cell nuclear transfer ( SCNT ) is likely to affect the expression level of growth-related genes especially imprinting genes .

体细胞核移植过程有可能影响克隆动物生长相关基因尤其是印迹基因的表达水平。

Sheep fetal fibroblast cells were transfected and screened . The cells which expressed exogenous genes stably was used as donor cells for SCNT .

转染并筛选绵羊胎儿成纤维细胞得到稳定表达外源基因的转基因细胞系，将其用作体细胞核移植的供体细胞。

Results showed the activation rates of SCNT embryos derived from electrofusion and injection method were 90.3 % and 87.3 % respectively ( P > 0.05 ) .

通过对电融合和注射方法获得的克隆胚胎对比，发现2种克隆胚胎在活化率上没有显著差异（87.3%VS90.3%，P>0.05）。

Animal production by somatic cell nuclear transfer ( SCNT ) offers a range of opportunities in basic and applied research , in agriculture , genetic conservation and human medicine .

体细胞核移植动物的生产，在基础理论研究和应用研究方面，如农业、遗传资源的保护和人类医学等，均具有广阔的应用前景。

In conclusion , some differential methylation sites were identified in SCNT cattle , the significance of which on the abnormal development of cloned animals need to be further investigated .

总而言之，本研究在体细胞核移植牛的基因组上鉴定了几个差异甲基化位点，但这些位点与体细胞克隆动物发育异常的关系还需做进一步研究。

Somatic cell nuclear transfer ( SCNT ) is a key technique for producing transgenic mammalian , so optimization of this technique is important for improving the total efficiency of transgenic animal production .

体细胞核移植技术为生产转基因动物核心技术之一，该技术的优化和成熟，对于成功生产转基因动物至关重要。

The technique of somatic cell nuclear transfer ( SCNT ) has many advantages when used in transgenic animal production , such as increasing the number of transgenic animal rapidly without losing foreign gene .

体细胞核移植技术在制备转基因动物方面有许多优点，例如：可以快速扩大转基因动物的群体，且能保持外源基因稳定传递。

Somatic cell nuclear transfer ( SCNT ) technology has been improved steadily in recent years , but the efficiency of nuclear transfer is still low , which limits the application of SCNT technology .

体细胞核移植技术在多年的研究发展中日益完善，但核移植效率仍然较低，限制了体细胞核移植技术的应用。

This research aim to establish an efficient system for producing pigs by somatic cell nuclear transfer ( SCNT ) . Effect of hormone 、 pFF and EGF on maturation of porcine oocytes have been investigated .

本研究旨在建立一套高效的体细胞克隆猪的生产体系。研究了激素、猪卵泡液及表皮生长因子对猪卵母细胞体外成熟的影响。

In this paper , we mainly focus on the abnormal development and expressing characteristics of " imprinting gene " in reconstituted embryos from porcine somatic nuclear transfer , including its applications and approach of increasing the efficiency of SCNT .

本文主要对猪体细胞核移植重构胚发育异常及印迹基因表达特征作一综述与分析，旨在为研究者提供一些有益的启示。

In conclusion , porcine fibroblasts were effectively synchronized at G0 / G1stages of the cell cycle by serum starvation or culturing to confluence , and there have no different on reconstructed embryos development while used as porcine SCNT donors . 2 .

结果表明，猪成纤维细胞通过血清饥饿法或完全汇合培养法均能有效的将细胞周期同期化至G0/G1期。