dotap

AS-ODN / DOTAP complex could exhibited more higher transfection efficiency than free AS-ODN and the transfection efficiency was also influenced by many other factors such as cell density etc.

DOTAP复合物的转染效率明显高于游离AS-ODN；细胞是否贴壁生长及贴壁生长的细胞密度也影响AS-ODN的转染效率。

In the present study , we prepared DOTAP / DOPE liposomes , characterized the interactions between these cationic liposomes and plasmid DNA by physicochemical researches , and studied their transfection efficiency in vitro .

在本研究中，我们制备了DOTAP/DOPE脂质体，对其与DNA的相互作用进行了理化表征，并研究了它们的体外转染效率。

In conclusion , complexes were formed between DOTAP / DOPE cationic lipid carriers and DNA through electrostatic force , ternary complexes were obtained after adding protamine sulfate . Four kinds of complexes could delivery DNA vaccine in vitro and in vivo .

综上所述，DOTAP/DOPE阳离子脂质体、阳离子脂质乳剂与DNA通过静电作用自组装形成复合物，分别加入鱼精蛋白后得到三元复合物，这4种复合物均能介导基因的体外转染和体内表达。