elispot

ELISPOT Assay to Detect Specific Reaction of T Cells to EBV Peptide Segments for Patients with Nasopharyngeal Carcinoma before and after Radiotherapy

酶联免疫斑点法检测鼻咽癌患者放疗前后T细胞对EB病毒肽段的特异性反应

Activated TH cells which secreted cytokines were detected by ELISPOT , and those which had red spots in their plasma after staining were positive cells .

采用酶联免疫斑点法检测活化的分泌细胞因子的TH细胞；

Technical optimization for detecting the antigen specific CTL immune response in ELISPOT assay

酶联免疫斑点法检测抗原特异的CTL免疫应答试验条件的优化

ELISPOT Detection of Cell Immunity in Over-Loaded Rats with Incremental Exercise

固相酶联免疫斑点技术检测递增负荷过度训练大鼠细胞免疫机能变化研究

There was an inhibition of antigen specific antibody production in pregnancy examined by ELISPOT .

脾脏单个核细胞在妊娠晚期分泌较少的抗原特异性抗体。

Application of ELISPOT and FACS in cellular immune response in patients with chronic hepatitis B virus infection

ELISPOT及FACS在慢性乙型肝炎病毒感染者细胞免疫功能检测中的应用

Cellular immune response between enhanced hepatitis B vaccine and normal hepatitis B vaccine by Elispot were compared .

同时将增效乙型肝炎疫苗与常规乙型肝炎疫苗进行比较。

Kinetics of rBCG_initiated T Cell Immune Responses Monitored by ELISPOT Assay

应用ELISPOT试验测定重组卡介苗诱导的T细胞应答

By ELISA , FCM , ELISPOT , the vaccine immune response effect in Balb / C mice were evaluated .

采用间接ELISA法、流式细胞术、ELISOPT技术，对疫苗在Balb/C小鼠体内的免疫应答效果进行了评估。

T-Lymphocytes Immune Function of IFN-Gamma Production from Tuberculous Pleural Effusions Assayed by ELISPOT

酶联免疫斑点法检测结核性胸水T淋巴细胞分泌IFN-γ的免疫功能

Methods Specific and non specific lymphocyte proliferation test and enzyme linked immunospot ( ELISPOT ) assay were used in the present study .

方法对口服CⅡ致耐大鼠进行特异和非特异性淋巴细胞增殖实验，并采用酶联免疫斑点法（ELISPOT），对CⅡ特异性T细胞在单细胞水平进行细胞内细胞因子检测。

The humoral response against the foreign gene has been detected using Elisa and ELISpot test is ongoing for the cytotoxic responses detection .

通过Elisa检测了针对外源基因的抗体反应，现在正在进行ELISpot试验检测免疫反应。

Detection of mouse immunity induced by HIV transmembrane modified DNA vaccine combined with poxvirus by ELISPOT and its specific CTL function

ELISPOT法检测HIV跨膜修饰的DNA疫苗与痘病毒联合免疫小鼠，其特异性CTL功能的研究

Methods IFN γ secreting cells and AChRAb secreting cells were detected in 22 cases of MG patients by ELISPOT assay .

方法采用免疫酶点方法检测22例重症肌无力患者外周血AChRAb分泌细胞和IFNγ分泌细胞。

Objective : To analyze the diagnostic utility of Enzyme-linked immunospot assay ( ELISPOT ) in children with tuberculosis in XinJiang area .

目的：探讨酶联免疫斑点技术（ELISPOT）对新疆地区小儿结核病的诊断价值。

Detection of LOS-specific antibody-secreting cells by ELISPOT assay

LOS诱导的特异性抗体分泌细胞的ELISPOT法检测

Blood sample was collected , antibody subtypes against HIV were detected , and mice were killed and cellular immune response was examined by ELISPOT at 10 week .

在第10周采集血清样本进行抗HIV抗体亚型检测，然后处死小鼠检测小鼠的细胞免疫水平。

Through lymphocyte proliferation experiment and ELISPOT , the group of nasal tolerance could be obviously suppressed , compared with the control ( P < 0.001 ; P < 0.01 ) .

淋巴细胞增殖实验显示，高、低剂量耐受组与对照组相比均可抑制特异性淋巴细胞增殖反应（P<0·001；P<0·01）。

By ELISPOT and reverse hybridization with PCR-oligonucleotide probes techniques on the diagnosis of spinal tuberculosis , individualized treatment pattern of drug resistance spinal tuberculosis has better prospects .

综上所述，以ELISPOT、PCR-寡核苷酸探针反向斑点杂交实验室诊断技术为基础，耐药脊柱结核个体化诊疗模式有较好的应用前景。

Results The cellular immune response in vaccined group was 5 / 5,4 / 5 and 3 / 5 by using ELISPOT , MHC-Pentamer and ICCD respectively .

结果ELISPOT、MHC肽五聚体染色以及胞内细胞因子染色分析法检测疫苗组细胞免疫应答的阳性率分别为5/5、4/5和3/5；

Objective To compare enzyme-linked immunospot assay ( ELISPOT ) and tuberculin skin test ( TST ) and explore their roles in the auxiliary diagnosis of initial pulmonary tuberculosis .

目的比较酶联免疫斑点检测（ELISPOT）和结核菌素试验（TST），探讨ELISPOT在初治肺结核中的辅助诊断价值。

Conclusion The specific cellular immunity in the patients with hepatitis B could be evaluated by determining the number of γ - interferon secreting cells with the method of testing their peripheral blood mononuclear cells by ELISPOT .

结论通过ELISPOT检测乙肝患者外周血单个核细胞γ-干扰素分泌细胞的数量可以了解乙肝患者特异性细胞免疫功能。

ELISPOT results showed that ,Δ BAP31 / GST fusion protein can stimulate the secretion of spleen cells in experimental group-specific IFN - γ, and the levels were significantly higher ( P 0.05 ) .

ELISPOT结果表明，？BAP31/GST融合蛋白可刺激实验组脾细胞分泌特异性IFN-γ，且水平显著高于对照组（P0.05）。

ELISPOT experiment in screening for LTBI , not BCG vaccination effect , strong specificity , no harm to human body , easy to medical personnel to accept , to get the real survey data . 3 .

ELISPOT实验在筛查LTBI方面，不受BCG接种影响，特异性较强，不会对人体造成伤害，易于医务人员接受，利于获得真实的调查数据。

Balb / c mice were immunized and the antibody titer was tested by ELISA . On the other hand , the T cell response was tested in vitro through ELISPOT and LDH-release assay .

免疫小鼠，ELISA法检测抗体，ELISPOT法和LDH释放法体外检测T细胞免疫反应活性。

Antibody-secreting cells ( ASC ) to β - Bungarotoxin binding protein from peripheral blood , bone marrow and thymus were investigated in 12 early engaged myasthenia gravis ( MG ) patients by ELISPOT assay .

应用ELISPOT方法检测了12例早期发病行胸腺切除术的重症肌无力（MG）患者的外周血、骨髓和胸腺的β-BGT结合蛋白抗体。

Objectives : The newly developed Interferon-gamma Release Assay ( IGRA ) is a new Enzyme-linked Immunospot ( ELISpot ) technology based on two tuberculosis-specific antigens , providing a fresh direction for the diagnosis of tuberculosis .

目的：近年来发展起来的基于结核分枝杆菌特异性蛋白的γ干扰素释放试验（Interferon-gammaReleaseAssay，IGRA）作为一种新型的酶联免疫斑点技术，为结核病的诊断提供了新的技术。

Methods We assayed specific antibody levels in serum , saliva and fecal extract by ELISA , antibody secreting cells in gastric mucosa and PP by ELISPOT and cytokines mRNA expression level of T cells in PP by RT-PCR assay .

方法ELISA分析免疫小鼠血清、唾液、粪便提取物特异抗体水平，ELISPOT分析胃粘膜、派伊尔小结（PP）抗原特异性抗体分泌细胞（ASC），RT-PCR分析PPT细胞因子mRNA表达水平。

Cytokine production was assayed by ELISPOT , and CD4 ~ + IFN - γ ~ + T helper cells or CD8 ~ + IFN - γ ~ + cytotoxic lymphocytes ( CTL ) were detected by flow cytometry .

小鼠脾淋巴细胞细胞因子的产生用ELISPOT方法检测，CD4~+IFN-γ~+T辅助细胞和CD8~+IFN-γ~+细胞毒T淋巴细胞（CTL）用流式细胞仪进行检测。

Conclusion : ELISPOT is sensitive and fast to diagnose childhood tuberculosis and is less affected by factors such as malnutrition and severe tuberculosis . Owing to having better diagnostic value , ELISPOT can be applied in the early diagnosis of childhood tuberculosis in Xinjiang area .

结论：ELISPOT诊断小儿结核病敏感、快速，而且不受营养不良、重症结核病因素的影响，具有较高的诊断价值，可用于新疆地区小儿结核病的早期快速诊断。