中期染色体

显微镜光度计扫描测量人中期染色体的面积与DNA相对含量

Photometric Scanning of Area and Relative Content of DNA in Human Metaphase Chromosome with a Microscope Photometer

几株细胞中期染色体NORs与间期核AgNORs活性比较

Comparision of the activity between metaphase chromosome NORs and interphase nucleus AgNORs in some cell lines

水稻中期染色体和DNA纤维的高效制备技术

The Highly Efficient Methods for Preparation of Rice Metaphase Chromosomes and DNA Fibres

水稻间期核、粗线期和有丝分裂中期染色体FISH分辨率的比较

Comparative Study of FISH Resolution on Interphase , Pachytene and Metaphase Chromosomes in Rice

有多个形态结构完整的染色体，但染色体在整个生命周期中永远处于高度凝集状态，DNA纤维的折叠方式与典型真核生物中期染色体完全不同；

There are many chromosomes in the nuclei , but the chromosomes remain permanently condensed during cell cycle ;

小鼠成纤维细胞早中期染色体标本制备及G带核型分析

The Methods of Preparing Early Metaphase Chromosomes and the Study on Their G-banding Patterns in Mouse Fibroblasts

核糖体DNA（rDNA）在六种植物中期染色体上的原位杂交定位

In situ Localization of rDNA to Metaphase Chromosomes in Six Species of Plants

用电镜原位杂交技术对玉米中期染色体中RNA的研究

Studies on RNA in the metaphase chromosome of Zea mays by electron microscopy in situ hybridization

目前，这两个技术还有很多不足，该研究建立了高效制备水稻中期染色体和DNA纤维的方法。

However these techniques are still in deficiency at present . In this study , a method to prepare metaphase chromosomes with high efficiency was deve-lopped in rice .

利用加入氨甲喋呤和胸苷使细胞分裂同步化并结合胰酶G带技术，分析了猪前中期染色体高分辨G带。

Analysis of high resolution G bands of prometaphase chromosomes in pigs was carried out by cell synchronization using amethopterin and thymidine treatment combining GTG technique .

本文利用RNase－Gold标记法对K＿（562）细胞中期染色体内RNA的分布特点进行了电镜观察，发现RNA较均匀地分布于染色单体切面内部及边缘。

In the paper , we have studied the RNA distribution in the metaphase chromosomes of K_ ( 562 ) cell using RNase-gold method .

将上述定位的鸡的20个功能基因的BAC克隆分别与利用胚胎成纤维细胞制备的鸭和鸵鸟的中期染色体杂交。

The above BAC clones of20 chicken genes were hybridized by FISH with duck and ostrich metaphase chromosomes prepared from embryo fibroblasts .

有丝分裂中期染色体上的CPD带纹与粗线期染色体上显著的带纹具有对应性。

The CPD bands exhibited on mitotic metaphase chromosomes corresponded to the prominent bands exhibited on the pachytene chromosomes .

采用传统的核型分析技术，分析了栽培丹参有丝分裂中期染色体的核型和C-banding。

Karyotype and C-banding of chromosomes of Salvia miltiorrhiza at mitosis metaphase were analyzed with traditional methods .

应用LM（光镜）与SEM（扫描电镜）观察同一病例非显带和不同程度G显带的人体中期染色体，证明染色体固有的大体形态相同，而表面结构有显著的差异。

Observing the non-banded and G-banded human metaphase chrom - somes of the same patient by the LM and the SEM , It was evident that chromosomes specific gross morphology was the same .

粗线期SC染色体C-带、G带显示了较为清晰的带纹，但带纹沿染色体纵长基本均匀分布，而早中期染色体C-带、G-带显带效果不理想；

Synaptonemal complexes in meiotic pachytene displayed conspicuous C-banding and G-banding , but the bandings regularly distributed along the lengthwise chromosomes . The results of C-banding and G-banding in mitotic early metaphase was not clear .

CPD染色在8条粗线期染色体上显示出了10条红色的CPD带纹，在6对有丝分裂中期染色体上显示出了12条CPD带纹。

Ten red CPD bands were shown on eight pachytene bivalents , and 12 bands were shown on six pairs of mitotic metaphase chromosomes .

为了快速检测特异性染色体，采用引物原位标记和细胞化学技术，以7号，17号，X和Y染色体特异性寡核苷酸作引物，对外周血淋巴细胞分裂中期染色体和间期核进行定位研究。

In order to identify specific chromosomes rapidly , with specific primers for chromosome 7 , 17 , X and Y , both metaphase and interphase nuclei of peripheral blood lymphocytes were detected by primed in situ ( PRINS ) labeling technique .

1例间期细胞FISH与羊水培养结果一致，另1例羊水细胞培养仅收到很少分裂中期染色体，改做FISH后可见到较好的荧光信号。

The result from one case with interphase cells by FISH was coincidence with it by amniotic cell culture . The other case got a little metaphase chromosomes by amniotic cell culture , and then got better signals in interphase cells by FISH .

【结果】在大白菜中期染色体上，分别检出了5对25Srdna杂交信号和3对5Srdna杂交信号。

【 Result 】 Five pairs of hybridization signals of 25S rDNA and three pairs of hybridization signals of 5S rDNA were detected on the metaphase chromosomes .

应用荧光原位杂交技术进行45Srdna在不同倍性沙田柚中期染色体上的定位研究。

Chromosome localization of 45S rDNA was studied by means of fluorescence in situ hybridization ( FISH ) on metaphase chromosomes of different ploidy Citrus grandis .

应用细胞融合技术和扫描电镜研究了BK（牛肾）细胞早熟凝集染色体（PCC）和诱导PCC的CHO中期染色体的超微结构。

The ultrastructure of prematurely condensed chromosomes ( PCC ) in BK cells and the CHO metaphase chromosomes ( the PCC inducer ) were studied with cell fusion technique and SEM .

方法：分别应用Giemsa法和染色体着丝粒点&核仁组织区（Cd-NOR）同步银染法对人类外周血淋巴细胞中期染色体标本进行染色。

METHODS : Giemsa method and Simultaneous silver staining technique of both NOR and Cd are used to stain metaphase chromosome specimen of human outer circular blood respectively .

方法应用原位PCR的方法对豫医无毛小鼠中期染色体标本的无毛基因进行检测，并设立PCR反应液中无taqDNA聚合酶、无引物、无bio-11-dUTP等进行多组对照。

Methods Slides of metaphase chromosome were examined by the method of PCR in situ . And PCR reactions without Taq DNA polymerase , primer and bio 11 dUTP were set up as control groups .

用改良的CPD染色程序清晰而稳定地显示出这些特征性的CPD带纹为番茄的染色体，特别是有丝分裂中期染色体提供了新的识别标记。

The distinctive CPD bands , which could be constantly and clearly detected using the CPD staining procedure we improved , provided new landmarks for chromosome identification in tomato .

【方法】用荧光原位杂交技术对25Srdna和5Srdna在大白菜有丝分裂中期染色体进行了定位研究。

【 Method 】 Fluorescence in situ hybridization ( FISH ) technique was used to locate the 25S rDNA and 5S rDNA on the mitotic metaphase chromosomes of Chinese cabbage-pe-tsai .

采用Sato对植物细胞银染的方法对大蒜中期染色体的超微结构进行了研究。

The mitotic metaphase chromosomes of garlic cells were studied at the ultrastructural level with silver ac - cording to Sato about Ag-staining technique devised for plant cells .

采用CPD（PI和DAPI组合）染色对番茄减数分裂粗线期和有丝分裂中期染色体进行了显带分析，随后用两种不同的45Srdna克隆在相同的分裂相进行了荧光原位杂交定位分析。

In this study , we performed sequentially combined PI and DAPI ( CPD ) staining and FISH with two different 45S rDNA clones on meiotic pachytene and mitotic metaphase chromosomes in tomato .

BrdUrd-Hoechst-Giemsa技术在两栖类的中期染色体上能显示可重复的复合带型。

Metaphase chromosomes showing multiple banding pattern were easily reproducible using BrdUrd-Hoechst-Giemsa technique in Amphibia .

本文应用分离染色体骨架的TEM技术、超薄切片甩镜细胞化学、RNase一胶体金标记和银染电镜技术结合研究了牛肾（BK）细胞的分离染色体骨架和完整的中期染色体骨架。

The isolated or intact metaphase chromosome scaffold of BK ( Bovine Kidney ) cell has been studied by using several techniques , such as isolated chromosome scaffold TEM , ultra thin-sectioning cytochemical EM , RNase-gold complexes labeling and Ag-staining .