冈比亚按蚊
- 网络An. gambiae;A. gambiae;anopheles gambiae;An.gambiae
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该研究组让冈比亚按蚊携带有一种绿色荧光基因,让它们在黑暗中发光。冈比亚按蚊是疟疾最重要的传播者。
The team bred Anopheles gambiae mosquitoes , the most important carriers of malaria , to contain a green fluorescence gene that makes them glow in the dark .
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目的探讨冈比亚按蚊中肠抗原抗媒介免疫的作用,进一步研究抗媒介疫苗抗原。
Objective To examine the anti vector immune response of midgut from the mosquito , Anopheles gambiae .
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方法根据已发表的埃及伊蚊和冈比亚按蚊防御素基因序列设计合成两对引物,以中华按蚊成蚊cDNA为模板进行PCR扩增,将预期片段克隆测序并进行分析;
Methods Two pairs of primer were designed and synthesized based on the published defensin sequences , and PCR amplification was performed using the cDNA of An . sinensis as the template .
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蚊虫的防御素基因首先从埃及伊蚊中克隆,2000年Eggleston等报道了冈比亚按蚊防御素基因的DNA结构及其免疫调节作用。
The defender genes of mosquitoes firstly clone from aedes . Eggleston ( 2000 ) reports DNA structure of the defender genes of anopheles and its immune regulation .
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其中9个与LPS诱导的冈比亚按蚊免疫反应性细胞系EST同源,占39.1%(9/23)。
Nine of the 23 genes ( 39.1 % ) also hit homologous sequences in the An . gambiae EST database generated from an immune competent cell line treated with lipopolysaccharide ( LPS ) .
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结果PCR扩增产物大小约270bp,目的基因的序列与冈比亚按蚊防御素基因同源性为85%;
Results The PCR product 270 bp in size was identified as the coding sequence for defensin after sequence analysis , which had 85 % homology with the defensin sequence from An .
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非洲冈比亚按蚊亚群种有6种外表看上去完全一样的蚊子,这对它们可是面临的一个问题。
That 's a problem that faces the Anopheles gambiae complex of mosquitoes , a group that comprises six identical-looking species .
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例如,冈比亚按蚊在小型泥浆水塘中繁殖,而这种水塘是由诸如掘井等人类活动造成的。
A.gambiae , for example , breeds in small pools of muddy water formed by human activities , such as well-digging .
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该项研究是全球卫生重大挑战项目子项目的组成部分。该子项目旨在开发一种化学策略防止冈比亚按蚊传播疟疾。
The research is part of the Grand Challenges in Global Health Initiative to develop a chemical strategy to prevent the spread of malaria by A.gambiae .
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卵囊黑化包被反应是按蚊抵御疟原虫感染的特异性免疫防御机制,也是抗性株冈比亚按蚊或不易感按蚊的最主要表型。
The melanotic encapsulation of oocysts is a specific immune defence mechanism against Plasmodium infection , and is also a primary phenotype in the Plasmodium-refractory strain of An .