四聚体

MHC-肽四聚体技术是近年来建立的检测特异性CTL的有效方法。

MHC peptide tetramer is a sensitive method to evaluate antigen specific CTL .

MHC-肽四聚体技术在CTL表位鉴定中的应用

Application of MHC-peptide Tetramer Technology in Epitope Identification

富含B细胞的淋巴细胞标本用三种不同的HLA四聚体中的1种或多种进行染色。

Lymphocyte preparations enriched for B cells were stained with one or more of three different HLA tetramers .

HIV-1整合酶四聚体结构模拟及其活性位点分析

Modeling the Tetrameric Structure and Its Active Sites of HIV-1 Integrase

LDH只有1个基因位点，为四聚体酶；

LDH , 1 locus and 2 alleles ;

结论：HLA四聚体染色法为HLA特异性B细胞的鉴别、定量、及分离提供了一种新的手段。

CONCLUSIONS : Staining with HLA tetramers provides a means for identifying , quantifying , and isolating HLA-specific B cells .

甲基CpG结合域四聚体蛋白的原核表达、纯化及鉴定

Prokaryotic expression , purification and identification of tetrameric-protein of methyl-CpG-binding domain

采用四聚体技术，运用流式细胞仪检测病毒特异性CTL。

Flow cytometry was used to exam viral specific CTL .

结论我们所建立的四聚体技术可定量检测表位特异性CTL；

Conclusion Tetramers in our experiments could quantitatively detect epitope-specific CTL activity .

该DNA分子马达是一条具有特定序列的富含胞嘧啶的核酸片段，其构型对pH敏感，在酸性溶液中为四聚体构型，在碱性溶液中为直链构型。

The DNA-motor is a nucleic acid fragment with a specific cytosine-rich sequence , and it forms i-motif structure in acidic solution and straight-chain structure in alkaline solution .

HBc-HLA-A~1101-β2m复合物单体及其四聚体的制备和鉴定

Preparation and identification of HBc-HLA-A ~ 1101 - β _2m complex monomer and tetramer

抗前列腺特异抗原/抗CD3双特异性单链抗体四聚体的活性研究

Biological activity of tetramer of bispecific single-chain antibody against human prostate-specific antigen and CD3 molecule

流式细胞仪分析显示所制备的四聚体可与HLA-A11+供者的抗原特异性CTL结合。

Flow cytometry analysis indicated that the tetramer could bind to specific CTL from HLA-A11 + donor .

应用HIV-1特异性表位多肽HLA-Ⅰ类分子四聚体分析HIV-1感染者体内特异性CTLs的研究

Using HIV-1 Antigen Peptide-specific and HLA-class ⅰ - restricted Tetramers to Characterize CTLs in HIV-1-infected Individuals

应用H2Kb四聚体对特异性CTL检测结果与经典的细胞毒试验一致，同时对胞外IFNγ进行了定量检测。

Specific CTL responses were demonstrated by H 2K b OVA tetramer staining and cytotoxicity assay . Extracelluar IFN γ was also quantitatively analyzed .

目的：研究抗前列腺特异抗原（PSA）/抗CD3双特异性单链抗体四聚体的生物活性。

AIM : To study the biological activity of tetramer of bispecific single-chain antibody against human prostate-specific antigen ( PSA ) and CD3 molecule .

本实验为下一步构建AChRα四聚体并作为抗原检测乙酰胆碱受体抗体奠定了基础。

This experiment lays the foundation for constructing tetrameric AChR α to be used as antigen in the determination of anti-AChR antibodies .

同时，MDH多为同源二聚体或四聚体结构，也可以作为研究寡聚蛋白结构和功能的理想模型蛋白质。

For its homo-dimer or tetrad structure , MDH is regarded as an ideal model for research on oligomeric protein structure and function .

应用SDSpage和凝胶过滤层析对分子量的测定，提示该酶是由4个分子量为110～115kD的相同亚基组成的同源四聚体。

It was estimated that the purified hydrolase was consisted of four identical subunits with a molecular mass of 110 ~ 115kD by SDS-PAGE and gel filtration chromatography .

结论：通过对染色条件进行优化可明显提高四聚体染色的特异性，降低非特异性结合，优化的四聚体染色法能用于抗原特异性CTL的表型和功能分析。

CONCLUSION : The optimized tetramer staining condition can increase the specificity of tetramer staining and decrease unspecific binding , therefore it is applicable for phenotyping and functional analysis of antigen-specific CTL .

结论HLA肽四聚体流式细胞技术能在体外直接检测HBV特异性的CD8+T细胞数量，其水平一定程度上反映了特异性CTL应答状况，且与乙肝的不同临床转归有关。

Conclusion HLA-peptides tetramer staining flow cytometry is a direct exvivo method to detect the lever of circulating HBV specific CD8 + cells which reflects the response of CTL and relates to clinical consequence in hepatitis B patients .

目的构建并表达了人可溶性单链β2m-HLA-A2-BSP（scHLA-A2）融合蛋白表达载体，为进一步制备HLA-A2四聚体及其功能研究奠定了基础。

Objective To construct and express the human soluble single chain β 2m-HLA-A2-BSP fusion protein expression vector .

方法应用流式细胞计数仪辅以荧光标记组织相容性抗原Ⅱ类分子四聚体染色，从自发性糖尿病的NOD小鼠和非糖尿病的BALB/c小鼠中分离出谷氨酸脱羧酶（GAD）多肽特异性T细胞。

Methods Glutamic acid decarboxylase ( GAD ) - peptide specific T cells with fluorescein-labelled Class ⅱ MHC tetramers were isolated by flow cytometer from NOD mice with spontaneous diabetes and diabetes resistant BALB / c mice .

MHC-肽四聚体（Peptide-MHCtetramer）技术是免疫学和临床诊断中重要的技术，广泛应用于免疫学研究及免疫性疾病临床研究。

Peptide-MHC tetramer is the key technology of Immunology and Clinical diagnosis , and has been widely used in immunology research and immunological disease clinical research .

而过去检测CTL的方法都是间接的，最近发展起来的四聚体技术则是直接检测抗原特异性CTL的有效而特异的方法，成为目前研究T细胞免疫应答的关键技术。

Previously used methods for detecting specific CTL are usually indirect . In recent years , tetramer technology has been developed to directly visualize antigen-specific CTL efficiently , and become the critical approach in studying T cell immune responses .

结论：通过与传统方法作比较，证实此法折叠效率比传统方法高，为进一步HLA肽四聚体及人工抗原提呈细胞的制备可提供足够可溶性的HLA-A2肽单体。

CONCLUSION : This study confirmed that the refolding efficiency of the method reported in this paper is higher as compared with the conventional method , which is of importance to the preparation of HLA-peptide tetramers and artificial antigen presenting cells .

实验获得了高纯度的在体外构建鸡MHC-肽四聚体所需的重组蛋白BF2-BSP。

All these results indicated that high quality of recombinant protein of BF2-BSP was obtained , which will be contributed to the construction of chicken MHC peptide tetramer .

生物素化的HLA-A0201/肽复合物单体与荧光素（phycoerythrin或allophycocyanine）标记的链霉亲和素（含4个生物素化位点）按4∶1的比例结合，形成可溶性HLA-A0201/PR1四聚体。

Biotinylated HLA-A0201 / peptide complex monomer was labeled with streptavidin ( including four biotin sites ) fluorescein ( phycoerythrin or allophycocyanine ) by the ratio of 4:1 to form soluble HLA - A 0201 / PR1 tetramer .

卤代甲烷在一维四聚体水簇上分布的理论研究

Theoretical study on distribution for halogen methane in water tetramers

每一个血红蛋白分子是由4个亚基组成的，即为四聚体。

Each hemoglobin molecule consists of four sub units , known as tetramers .