密度梯度

方法：分别应用上游法、Percoll密度梯度离心法及双曲管优选法处理精液，观察优选前后精子染色体畸变率、精子性染色体比例及超微结构相对正常的精子百分率。

Methods : The selection techniques of swim-up , Percoll density gradient centrifugation and doule-flection tube were used to observe sperm chromosome abnormality rate , sex chromosomal ratio and the percentage of spermatozoa with relatively intact head ultrastructure .

快速密度梯度离心法纯化质粒DNA

Purification of plasmid DNA by rapid density gradient centrifugation

无外力场和密度梯度情况下的玻尔兹曼H定理

Boltzmann 's H Theorem Without External Force Field and Gradient of Density

MeanShift就是偏移的均值向量，指向概率密度梯度方向。

Mean Shift is the mean vector , direction of which is just the gradient of probability density .

方法细胞：采用密度梯度离心法，分离人脐血单个核细胞（MononuclearCells，MNCs）。分组：分为3组。

Method Human umbilical blood mononuclear cells ( MNCs ) were separated with density gradient centrifugation .

MeanShift算法是一种非参数的密度梯度估计算法，近年来将其运用到目标跟踪上，并取得了较好的跟踪效果。

Mean Shift algorithm is a non-parametric density estimation algorithm . It was used in object tracking recently years , and the effect was well .

密度梯度离心法获得大鼠MSCs，传代培养并扩增。

MSCs were obtained from SD rats using the method of density gradient centrifugation culture .

用密度梯度分离法分离并计数支气管肺泡灌洗液（BALF）中不同密度的嗜酸性粒细胞；

The eosinophils in BALF were separated by density gradient centrifugation .

结论1.经观察细胞形态和细胞表面标志物鉴定，采用密度梯度离心结合贴壁法可在体外成功分离、培养MSCs。

MSCs can be isolated by density gradient centrifugation and adherent culture in vitro . 2 .

结果：1.体外培养MSCs的形态特征经密度梯度离心的MSCs形态比较均一。

Morphological characteristics of MSCs in vitro by density gradient centrifugation of MSCs relatively uniform shape .

结果：Percoll密度梯度离心法培养可获得均一的MSCs；

RESULTS : Homogeneous MSCs were obtained by percoll density gradient centrifugation .

方法：采用多聚阴离子沉淀结合短时密度梯度超速离心法得到纯化的LDL；

Methods : To isolate LDL by using polyanions precipitation and short-time density gradient ultracentrifugation .

结论：用密度梯度离心和贴壁培养法可以分离并培养得到bMSCs。

Conclusion : bMSCs can be obtained from bone marrow by the density gradient centrifugation method .

不同于参数估计的方法，一旦确定了跟踪目标，MeanShift方法不需其他的额外输入，而且该方法有计算概率密度梯度的功能。

Different from parametric estimation , Mean shift does not need any other parameters once the target is defined . And it also can compute the gradient of probability distribution .

方法：选用54只雄性SD大鼠，密度梯度离心法分离骨髓间充质干细胞（MSCs）。

METHODS : Bone marrow MSCs were obtained by density gradient centrifugation from the tibiae of 54 male SD rats .

高速离心密度梯度法分离大鼠脑皮质线粒体，Clark氧电极法测定线粒体呼吸活性，极谱法测量COX活性。

Mitochondrial respiratory function and COX activity were measured by Clark oxygen electrode and polarography , respectively .

Percoll密度梯度离心分离不同发育阶段生精细胞的方法

Separation of Mouse Spermatogenic Cells by Centrifugation in Percoll Density Gradients

通过密度梯度离心与红细胞裂解后联合贴壁培养法可获取较为纯化的MSCs。

MSCs were purified by the method of split red blood cell , combining gradient density centrifugation and adhesion separation .

结论联合采用密度梯度离心和贴壁筛选的方法易于分离、纯化获得纯度较高的MSCs。

Conclusion : MSCs is easy to be isolated and purified by combined using density gradient centrifugation and plastic adherence .

结论：采用超滤、PEG沉淀及连续蔗糖密度梯度超速离心的方法可制备满足麻疹IgGEIA诊断试剂要求的包被抗原。

Conclusion : We can prepare the coating antigen in measles IgG EIA test system by continuous sucrose density gradient ultracentrifugation .

兔血清LDL、VLDL及HDL用密度梯度超速离心法分离得到。

The serum LDL , VLDL and HDL were isolated by the density gradient ultra centrifugation .

脊髓灰质炎病毒（Poliovirus）的Percoll等密度梯度离心分离

Poliovirus Separation by Centrifugation in Percoll Isopycnic Gradients

所有实验组病例和对照组均采取4毫升静脉血，肝素抗凝，密度梯度离心法分离PBMCs。

PBMCs were separated from 4 ml heparinized blood by density gradient centrifugation .

Percoll密度梯度离心法分离大鼠肺Ⅱ型上皮细胞

Isolation of Rat Type ⅱ Alveolar Epithelial Cells by Discontinuous Percoll Gradient Centrifugation

采用Percoll非连续密度梯度离心法纯化大鼠乳鼠心室肌细胞

Enrichment of cardiomyocytes from neonatal rat ventricular tissues by discontinuous percoll gradient centrifugation

研究方法：采用Percoll密度梯度离心法分离大鼠BMSCs，对所获得的细胞进行体外培养及鉴定。

Methods BMSCs were isolated by percoll solution from rat bone marrow sample , cultured and identified .

本文采用区带密度梯度离心，国内首次分离得到黄牛纯化的高密度脂蛋白（HDL）。

The bovine high density lipoprotein ( HDL ) was first isolated and purified in our country with zonal gradient ultracentrifugation .

方法：使用密度梯度法分离成年大鼠骨髓MSCs，以地塞米松、β-甘油磷酸钠、维生素C为成骨诱导剂。

Methods : MSCs were isolated from adult rat by using density gradient separation method . The osteo-genic inducers were compounds of Dexone , β - glycerophosphate sodium and vitamin C.

方法采用机械分离、酶消化和不连续密度梯度离心法分离提取TIL前体细胞；

Methods TIL were obtained through mechanical separation , enzyme digestion and discontinuous density gradient centrifugation .

血细胞的Percoll不连续密度梯度分离法

Discontinuous Density Gradient Centrifugal Method for the Human Blood Cell Separation Using Percoll Medium