硫酸铵沉淀

用棉铃虫组织蛋白酶B为酶源，通过硫酸铵沉淀、排阻层析和离子交换层析等方法，从大豆中分离纯化到了一种对HCB有抑制活性的蛋白酶抑制因子，命名为HCBSoyI。

An inhibitor , HCB_SoyI , purified from the soybean seeds by ammonium sulfate precipitation , gel filtration and ion_exchange chromatography , was here reported .

利用Taq酶的热稳定性，将破胞液在75℃水浴1h，然后用硫酸铵沉淀法获得纯化的表达蛋白。

Using the thermostable ability of Taq DNA polymerase , the protein was immerged in a 75 ℃ water-bath for 1h , and then obtained by the method of ammonium sulfate precipitation .

用硫酸铵沉淀法从液体培养物中纯化噬菌体λDNA

Purification by Ammonium Sulfate Precipitation of Bacteriophage λ DNA from LB-Broth Culture

经硫酸铵沉淀、DEAE-sepharoseFastFlow阴离子层析等步骤纯化了该重组表达蛋白。

The recombinant cellobiohydrolase ⅱ was purified by using ammonium sulfate fraction , DEAE-Sepharose Fast flow chromatography .

腹水单抗经辛酸-硫酸铵沉淀法纯化后，蛋白含量为4.84mg／ml。

The Mabs protein against porcine IgM from ascitic fluids prepared by caprylic acid / ammonium sulfate was high purified , with its concentration of 4.84mg/ml .

A30菌株培养液经硫酸铵沉淀、FPLC疏水色谱、HPLC反相色谱后，纯化得到一种拮抗活性物质，命名为P1。

An antagonistic substance produced by A30 was purified by ammonium sulphate precipitation , FPLC and HPLC and named P1 .

采用辛酸、硫酸铵沉淀法，纯化腹水中的mAb，以SDS-PAGE鉴定其纯度。

The mAbs in ascites were purified by caprylic acid-ammonium sulfate salting out and the purity of the mAbs was identified by SDS-PAGE .

方法采用硫酸铵沉淀、蛋白G亲和层析纯化尿中IgG，并经SDS-PAGE、Western印迹分析鉴定。

Methods Proteins were precipitated from urine with ( NH4 ) 2SO4 , and urinary IgG was purified by protein G column chromatography . SDS-PAGE and Western blotting were performed to analyze the urinary IgG .

本试验采用辛酸加硫酸铵沉淀二步法提纯兔抗EDS－76病毒血清中的IgG。

A method for the purification of IgG against Egg Drop Syndrome 1976 ( EDS 76 ) virus in rabbit with caprylic acid and ammonium sulphate precipitation was developed .

常规方法诱生腹水，辛酸-硫酸铵沉淀法和DEAE纤维素层析法对腹水进行纯化。

Ascites induced with conventional methods were purified by the methods of octanoic acid-ammonium sulfate precipitation and DEAE cellulose chromatography .

MBP亲和层析、Sepharose-4B凝胶层析、辛酸-硫酸铵沉淀法均不适用于纯化罗非鱼血清Ig。

Sepharose-4B gel chromatography , MBP affinity chromatography and octanoic acid-ammonium sulfate precipitation were not fit for purifying serum Ig of tilapia .

sobrinus6715免疫母鸡，采用硫酸铵沉淀法提取鸡蛋黄抗体，SDS-PAGE凝胶扫描鉴定抗体性质，分析其纯度并用分光光度法测蛋白质含量。

Egg yolk antibody IgY were isolated by sodium sulfate precipitating method and identified by SDS-PAGE . After electrophoresis the gel was scanned by chromatographic scanner and the protein content was evaluated by absorption spectrometry .

用饱和硫酸铵沉淀法纯化所收集腹水，得到纯化单克隆抗体，其蛋白含量为8.360mg/mL，用间接ELISA法测得该单克隆抗体效价为1：102400。

The purified ascites precipitated by saturated ammonium sulfate ( SAS ) with protein content 8.360mg/mL and the antibody titer reached 1:102400 determined by indirect ELISA .

通过85%硫酸铵沉淀和SephadexG-75凝胶层析将酸性α-淀粉酶粗酶液纯化了32.5倍，活力回收率为10.0%。

The crude amylase was purified 32.5 times and 10.0 % recovery of enzyme activity through 85 % saturation ammonium sulfate precipitation and Sephadex G-75 gel chromatography .

通过对纯化条件的摸索，将表达上清先后经过盐析（硫酸铵沉淀法）、CMSepharoseFastFlow弱阳离子层析、QsepharoseFastFlow强阴离子层析和BlueSepharoseFastFlow亲和层析四步纯化。

After optimizing the purification conditions , we used salting out ( ammonium sulfate precipitation ), CM Sepharose Fast Flow weak cation chromatography , Q Sepharose Fast Flow strong anion chromatography and Blue Sepharose Fast Flow affinity chromatography to purify the ES-HSA fusion protein successively .

结果经过硫酸铵沉淀和Octyl-Sepharose疏水层析纯化，annexinⅤ纯度达到95%。

Results The purity of annexin ⅴ reached up to 95 % after ammonium sulfate precipitation and octyl-sepharose hydrophobic interaction chromatography .

用经硫酸铵沉淀、高速离心并用蔗糖密度梯度离心提纯的鸡传染性法氏囊病病毒（IBDV）免疫BALB/C小鼠。

BALB / C mice were immunized with infectious bursal disease virus ( IBDV ) which had been purified with ammonium sulfate precipitation , high-speed centrifugation and ultracentrifugation in a sucrose gradient .

从半夏（Pinelliaternata）块根中，经95%硫酸铵沉淀和固定化猪甲状腺球蛋白柱亲和层析分离得到一种凝集素，称为半夏凝集素（PTL）。

Pinellia ternata lectin ( PTL ) has been purified from root tuber juice of Pinellia ternata by precipitation with 95 % saturation of ammonium sulfate and affinity chromatography on immobilized porcine thyroglobulin .

首先对钝顶螺旋藻中藻蓝蛋白的提取和纯化方法进行了改进。采用磷酸盐缓冲液循环冻融联合超生波破碎法，50%硫酸铵沉淀获得CPC，提取率达到13.1%。

Through repeatedly freezing and thawing alternatively in the presence of phosphate buffer combined with ultrasonic fragmentation , the CPC is efficiently extracted by being precipitated in 50 % ( W / V ) solid ammonium sulphated resulting in 13.1 % recovery of C-phycocyanin .

用匀浆提取、90%硫酸铵沉淀和CM-纤维素柱层析，SephadexG-150、G-100、G-75柱层的方法从猪心中制备了线粒体苹果酸脱氢酶。

By using homogenization extraction , 90 % ammonium sulfate precipitation and chromatogra-phy of CM-cellulose 、 Sephadex G-150 、 G-100 and G-75 , the m-MDH was prepared from pig heart .

方法将组分Ⅲ沉淀溶解，经柠檬酸钡吸附、洗脱、硫酸铵沉淀、2次DEAE-sepharoseFastFlow离子交换层析和SephadexG-100凝胶过滤纯化人FⅦ。

Methods The purification procedure of human factor ⅶ from Cohn fraction ⅲ precipitate involves dissolving fraction ⅲ, absorbing factor ⅶ onto barium citrate and eluting , ammonium sulfate fractionation and DEAE-Sepharose Fast Flow ion exchange chromatography , and Sephadex G-100 gel filtration chromatography .

采用一种经修改的硫酸铵沉淀及反向抽提法，从猪心肌粗提液中纯化得比活性为60u/mg的乳酸脱氢酶（LDH），纯化倍数为50倍，酶活性回收为78%。

A modified method of ammonium sulfate precipitation and back-extraction was used , lactate dehydrogenase ( LDH ) with the specific activity ( 60.09u / mg ) has been isolated from the crude extract of porcine cardiac muscle . This procedure yields a 50-fold purification with 78 % recovery .

发酵液经超滤浓缩、硫酸铵沉淀、阴离子交换色谱和分子筛色谱，获得了纯度为90%的SEC2，总收率23%。

SEC2 was separated and purified successively by ultrafiltration , salting out with ammonium sulfate , ion-exchange chromatography and molecular exclusion chromatography .

方法：从猪肾皮质中分离刷状缘微绒毛膜，用正丁醇溶解膜结合的酶，经硫酸铵沉淀，DEAEsephacel、ConASepharose、SephadexG200及抗γ谷酰基转移酶（GGT）免疫亲和层析提纯ALP。

Methods : The ALP purification procedure included isolation microvillus of porcine kidney cortices by solubilization the membrance-binding enzymes with 1-butanol , precipitation with ammonium sulfate , chromatography on DEAE-Sephacel , ConA-Sepharose , Sephadex G-200 and a immuno-affinity column of anti-GGT antibodies .

发酵液经过离心、超滤浓缩、硫酸铵沉淀、层析纯化后，得PHB解聚酶，纯化倍数为37.9，回收率11.81%。

The PHB depolymerase was separated and purified by centrifugation , ultrafiltration , ( NH4 ) 2SO4 salting out and gel filtration chromatography . The specific activity of the purified enzyme was increased by 37.9 folds over crude extract , and the recovery yield was 11.81 % .

以日本血吸虫可溶性成虫抗原（AWA）、尿素溶解性成虫抗原（JWU）、硫酸铵沉淀成虫抗原（AW）和可溶性虫卵抗原（SEA），用斑点免疫金银染色试验（Dot-IGSS）和Dot-ELISA诊断日本血吸虫病。

The AWA ( adult worm antigen ), JWU ( urea-soluble worm antigen from Schistosoma japonicum ) , AW ( ammonium sulfate precipitable worm antigen ) and SEA ( soluble egg antigen ) were compared for diagnosis of schistosomiasis japonica in Dot-IGSS ( dot-immuno-gold-silver staining ) and Dot - ELISA .

用硫酸铵沉淀法得到芸豆植物凝集素。

Ammonium sulfate deposition were used to get kidney bean phytohemagglutinin .

硫酸铵沉淀及反向抽提法纯化乳酸脱氢酶

Purification of lactate dehydrogenase by ammonium sulfate precipitation and back-extraction

硫酸铵沉淀尿酸酶的最佳浓度为75%。

75 % ammonium sulfate fractionation can get the most uricase activity field .

人血清性激素结合球蛋白结合容量的硫酸铵沉淀测定法及其临床应用

The measurement of binding capacity of sex hormone-binding globulin and its clinical application