神经丝

Alzheimer病及老年脑神经丝蛋白磷酸化异常

An Abnormal Phosphorylation of Neurofilament Proteins in Brains or Alzheimer ′ s Disease Patients and Aged Subjects

采用石蜡切片SP免疫组化方法检测两组标本中的神经丝蛋白及C-Kit的表达。

By using immunohistochemical SP method , neurofilament protein and C-Kit expression were detected . 3 .

研究表明：AD脑神经丝蛋白的糖基化修饰水平降低，伴随着磷酸化修饰水平增加，其机制目前尚不清楚。

The study demonstrated that O-Glycosylation of neurofilaments decreased and phosphorylation increased in AD brains .

目的：观察不同胎龄胎儿脊髓神经丝蛋白（NF）阳性神经元的形态、分布和发育变化，为脊髓-脊髓移植选择适宜的胎龄提供形态学依据。

Objective : To observe the shape and distribution of NF-like neurons in human fetal spinal cord .

醋酸铅对ST-8814细胞神经丝蛋白、微管结合蛋白mRNA水平的影响

Effect of lead acetate on mRNA expression of neurofilament protein and microtubule associated protein in ST-8814 cell line

方法建立体外培养小鼠耳蜗螺旋神经节细胞的方法，并进行神经丝蛋白（NF）免疫组化染色鉴定及受体酪氨酸激酶C（TrKC）免疫组化染色。

Methods The SGCs were cultured and purged in vitro . SGCs were identified by neurofilament ( NF ) and receptor tyrosine kinase C ( TrKC ) immunocytochemical staining .

免疫组化表明hMSCs在大鼠脑内表达人神经元特异性烯醇化酶（NSE）、神经丝蛋白（NF）和胶质纤维酸性蛋白（GFAP）；

Immunohistochemical staining showed that hMSCs expressed human neuron specific enolase ( NSE ), neurofilament ( NF ) and glial fibrillary acid protein ( GFAP ) .

过度磷酸化的神经丝是AD脑中NFT的一种主要成分和早期的病理改变，影响神经细胞的轴突转运功能。

Hyperphosphorylation of neurofilaments , one of major components of NFT and the early pathological change of AD , affects the function of axonal transport of nerve cells .

以免疫组化方法检测神经元特异性烯醇化酶（NSE）、神经丝蛋白（NF-M）、巢蛋白（nestin）、胶质纤维酸性蛋白（GFAP）的表达。

Neuron-specific enolase ( NSE ), neurofilament ( NF ), nestin , glial fibrillary acidic protein ( GFAP ) were detected by immunocytochemistry .

目的：从S-100蛋白和神经丝蛋白（neurofilament，NF）的不同时相分布，探讨益气化瘀方促进神经再生的机理。

Objective : To study the mechanism of the YI QI HUA YU recipe accelerate nerve regeneration by distribution of S-100 protein and neurofilament ( NF ) in different phase .

免疫组化染色选用的抗体分别为抗：①神经丝蛋白（NF-200），S-100蛋白。

The antibodies , which were used for the immunohistochemical studies , were produced against ( 1 ) NF-200 or S-100 protein ;

神经丝蛋白（NFP）阳性细胞和纤维在所检测的各个发育阶段均有表达，P53和P物质在各期胚胎视网膜均未检出阳性细胞。

The positive cells and fibers of neurofilament protein ( NFP ) appeared at all the stages of retinas but that of P53 protein ( P53 ) and Substance P ( SP ) were not found in all embryos .

结果：原代培养脊髓神经元中，神经丝蛋白的NF-H的表达，随细胞发育的逐渐成熟呈日趋上升趋势。

Results ; The expression of NF-H in primary cultured neurons of spinal cord increases correspondingly companied by cultured days .

结果：嗅球神经元可以在体外培养成功，且对神经元有特异性的标志蛋白抗神经丝蛋白（NFP）呈阳性反应。

The immunoreactivity of neurofilament pro ˉ tein ( NFP )( a specific marker protein for neurons ) was detected in the cultured neurons .

NF-H的发育特性及神经丝的完整性对NLY形状与分布的影响

The Developmental Characteristics of NF-H and the Effect of the Integrity of NF to the Shape and Distribution of NLY

原代培养脊髓神经元神经丝蛋白NF-H表达与免疫电镜观察

Study on Expression of Neurofilament-H ( NF-H ) in Primary Cultured Neurons of Spinal Cord and Its Distribution under Immuno-electron Microscope

做神经元特异性烯醇化酶、角质纤维酸性蛋白、神经丝蛋白免疫组化染色.结果：在加入成骨细胞分化培养液后7和14d钙钴法碱性磷酸酶染色阳性细胞百分率分别为11.5%和37.5%。

RESULTS : At the 7th and 14th day after osteoblastic induction , 11.5 % and 37.5 % of the cells stained with modified Gomori were positive , respectively .

诱导后5h，12h，1d，3d，7d在倒置显微镜下观察细胞形态，免疫组化鉴定神经丝蛋白、胶质纤维酸性蛋白的表达。

At 5 and 12 hours , 1 , 3 and 7 days after induction , cellular form was observed under invert microscope , the expressions of neurofilament protein and glial fibriliary acidic protein were identified with immunohistochemistry .

用参芪液诱导MSC分化为神经元样细胞，经免疫组化鉴定其神经元烯醇化酶（NSE）、神经丝蛋白（NF）、胶质纤维酸性蛋白（GFAP）的表达。

After MSC was induced to differentiate into neuron-like cells with Shenqi Liquid , expression of neuron-specific enolase ( NSE ), neurofilament ( NF ), and glial fibrillary acidic protein ( GFAP ) were detected by immunocytochemistry , respectively .

铅对仔鼠脑Brn-3a蛋白及神经丝蛋白表达影响

Effects of prenatal lead exposure on expression level of Brn-3a protein and neurofilament proteins in offspring rat brain

方法：动用Nissl染色显示小脑皮质神经元，免疫组织化学方法显示胶质纤维酸性蛋白免疫阳性（GFAP-IR）星形胶质细胞和神经丝蛋白免疫阳性（NF&IR）结构。

Method : Nissl staining was applied to exhibit cortical neurons ; immunohistochemical method was used to display GFAP-immunoreactive ( GFAP-IR ) astro-cytes and neurofilament-immunoreactive ( NF-IR ) neurons .

取培养7~14d内每天的及培养第3周、4周和8周时的脑片，固定、脱水、切片后行Nissl和抗神经丝重链（NFH）免疫组化染色。

After culturing 7 to 14 days , 3 weeks , 4 weeks and 8 weeks , respectively , cerebral slices were fixed , dehydrated and sectioned in cryostat . The sections proceeded with Nissl staining and neurofilament high molecular weight ( NFH ) immunohistochemical staining .

免疫定量结果表明：在小鼠生后14d，雌三醇可增加神经元特异性标记物神经元特异性烯醇化酶及神经丝的表达，而神经胶质细胞特异性标记物神经胶质纤维酸性蛋白不受影响。

Immune quantitative results showed that the expression of neuron specific markers , neuron specific enolase ( NSE ) and neurofilament ( NF ) were increasing with time until 14 days after birth . Expression of the neuroglial marker , glial fibrillary acidic protein ( GFAP ), was not affected .

大鼠弥漫性轴突损伤后神经丝68蛋白水平减少

Neurofilament 68 protein levels decreased after diffuse axonal injury in rats

神经丝蛋白质糖基化与磷酸化的相互调节和神经退行性疾病

Dynamic interplay between phosphorylation and O-glycosylation of neurofilaments in neurodegenerative diseases

结果：在海马结构内并不是所有的神经元均可被神经丝蛋白所标记。

Results : NF protein in the hippocampus only labeled some neurons .

人胚胎视网膜内神经丝蛋白的出现和分布

Occurrence and distribution of neurofilament protein in the retina of human embryos

实验性免疫介导的运动神经元损伤中神经丝异常的超微结构观察

Ultrastructural features of the abnormality neurofilaments in experimental immune-mediated motor neuron injury

一种神经丝研究方法的建立及应用

Establishment and application of a researching method for neurofilament

结论：并非海马结构所有神经元内或同一神经元的不同部位均存在神经丝蛋白。

Conclusion : NF protein only exists in some neurons or parts of a neuron .