纳豆

研究通过丹磺酰氯柱前衍生，用HPLC对纳豆发酵过程中的生物胺进行检测。

Biogenic amines during the fermentation of natto was detected by HPLC , using precolumn derivatization with DNS-Cl.

纳豆枯草芽孢杆菌基因组DNA提取后，经PCR扩增纳豆激酶及纳豆激酶原序列，并经1%琼脂糖凝胶电泳检测。

We extracted natto bacillus subtilis genomic DNA , PCR amplify the sequence , and detected by 1 % agarose gel electrophoresis .

经间接ELISA反应证实，所获得的纳豆激酶单克隆抗体是特异性针对纳豆芽孢杆菌的。

In indirect ELISA , two monoclonal antibodies were specific for Bacillus natto .

利用分光光度法对由纳豆菌发酵产生的纳豆激酶（NK）进行了动力学性质的研究。

The kinetics of Natto-kinase was studied by spectrophotometry .

纳豆芽胞杆菌16SRRNA基因的克隆及其系统进化分析

Cloning and phylogenetic analysis of 16S rRNA gene of Bacillus subtilis natto

报道了SD大白鼠口服纳豆芽胞杆菌一周后粪便菌群数量的变化情况。

The number changes of microorganisms in SD mouse 's intestines were reported after taking Bacillus natto Sawamura one week .

根据形态学特征、生理生化特征比较，确定纳豆芽孢杆菌为一株枯草芽孢杆菌（Bacillussubtilis）。

According to the morphological , physiological and biochemical characteristics , the natto bacillus is a Bacillus subtilis .

先培养纳豆菌8h，再加致病菌。

To cultivate bacillus for 8 hr then add pathogenetic bacteria .

E.coli中高效表达外源蛋白的探索及其在纳豆激酶中的应用

Study of the Method to Highly-Express Exogenous Protein in E.coli and It 's Application to Natto Kinase and Pro-Natto Kinase

纳豆生产的促生长因子为玉米浆3%、NaCl0.5%、无水葡萄糖2%；在4℃下后熟24h。

3 % corn paste , 0.5 % NaCl and 2 % anhydrous glucose were mixed as growth promoting factor .

N1型纳豆芽孢杆菌对泌乳期奶牛产奶量及乳品质的影响

Effects of Supplementation of Bacillus Subtilis Natto on Milk Production and Composition of Lactating Dairy Cow

经检测重组酵母发酵上清液中具纳豆激酶溶纤活性，SDS-PAGE电泳结果表明外源蛋白的表达量占菌体蛋白的10%左右。

The fibrinolytic activity was detected with the expression products of recombinant yeasts and the expression protein constituted 10 % of the total cell protein by SDS-PAGE .

纳豆激酶（Nattokinase，简称NK）是从日本传统食品纳豆中提取出来的具有溶血栓作用的一种酶。

Nattokinase ( NK ) is a fibrinolytic enzyme that is extracted from natto & a traditional food in Japan .

首先从纳豆中分离到一株能产NK的菌，经鉴定属于芽孢杆菌属（Bacillus），可能是纳豆菌（Bacillussubtilisnatto）。

It was identified to be included in the Genus Bacillus . There was a little difference between this strain and Bacillus subtilis . It may be Bacillus subtilis natto .

目的探讨纳豆菌与O157∶H7、金黄色葡萄球菌、沙门菌这3种致病菌分别共同培养时细菌数的变化情况。

Objective To study the change of bacteria number when Bacillus natto was cultured with O 157 ∶ H7 ? Staphylococcus Aureus ? Salmonella respectively .

利用Plackett-Burman法（PB法），以紫外分光光度法作为测量方法对产纳豆激酶枯草芽孢杆菌种子液培养条件进行了优化。

Plackett-Burman method ( PB method ) and UV spectrometer as testing method were used to optimize the culture conditions of natto kinase-producing Bacillus subtilis strain seed liquid .

纳豆激酶（NattokinaseNK）是纳豆发酵过程中由纳豆枯草杆菌（Bacillussubtilsnatto）产生的一种丝氨酸蛋白酶。

Nattokinase ( NK ) is produced by Bacillus subtils natto in the process of the fermentation of Natto which is a fibrinolytic serine enzyme .

本文采用纤维平板法测定纳豆激酶的活性，用美蓝染色法对传统的纤维平板进行了改进，并以尿激酶为标准，测定纳豆激酶发酵液酶活为7280（IU／mL）。

It is also discovered that Methylthioninium Chloride can be used to modified the traditional fiber plate . the fibrinolytic activity of the fermentation liquid is 7280IU / mg referred to the standard curve of Urokinase .

同时确定了重组纳豆激酶酵母菌的最佳甲醇诱导浓度2%，产酶高峰时间为96h。

Optimum methanol concentration of the P. pastoris recombinant engineering saccharomycete is 2 % and the period of time for activity peaks is 96 h.

用纳豆芽孢杆菌进行二次发酵最佳条件为：发酵温度36℃，接种量1%，发酵时间30h。

The optimum fermentation conditions for Bacillus natto were 36 ℃, 1 % inoculum size and 36 ~ 42 h fermentation time .

研究液体发酵产纳豆激酶的最佳条件，结果表明最佳培养基为营养肉汤，最佳培养时间为24h，最佳培养温度30℃。

The optimization of NK liquid fermentation was studied in this paper . The results showed that the optimal fermentation medium is nutrition meat broth , the optimal fermentation time 24 h , and fermentation temperature 30 ℃ .

结果：1.从纳豆中筛选出一株高产β-葡萄糖苷酶的细菌菌株，试验编号为NX-3。

From natto screened a high β - glucosidase of bacterial strains , the test number NX-3 .

经PCR扩增得到825bp的纳豆激酶成熟肽基因，采用pET32a（+）表达载体和BL21（DE3）为宿主菌，在温度为37℃、1‰IPTG浓度下可诱导获得较高表达量的重组纳豆激酶。

And SDS PAGE analysis revealed that using the pET32a ( + ) as expression vector and BL21 ( DE3 ) as host strain and cultivating at 37 ℃ with 1 ‰ IPTG , recombinant NK protein was highly expressed .

在统一饵料的基础上，分别按0.1%，0.2%和0.5%添加纳豆芽孢杆菌、枯草芽孢杆菌和复合微生态制剂养殖鲤鱼45d，分析了鲤鱼肠道和肝胰脏中蛋白酶和淀粉酶的活性。

Carps were fed with basal diet supplemented 0.1 % , 0.2 % , 0.5 % Bacillus subtilis , Bacillus natto and microorganism compounds for 45 days . The activities of protease and amylase in the intestine and hepatopancreas of carps were determined .

纳豆菌产抗菌物质培养条件的研究

Study on culture conditions of antimicrobial substances produced by Bacillus natto

纳豆激酶摇床发酵条件的优化

The Optimization on Fermentation of Natto-Kinase in Shake - Flask

产纳豆激酶枯草芽孢杆菌种子液培养条件的优化

Culture Condition Optimization of Strain Seed Liquid of Natto Kinase-Producing Bacillus subtilis

纳豆多肽蛋白粉制备工艺的研究

Study on the preparating technics of protein powder from natto

纳豆芽胞杆菌对大白鼠肠道菌群的影响

The Affect of Bacillus natto Sawamura to Intestine Microorganisms of SD Mouse

纳豆菌生产抗菌物质的研究

Studies on the antimicrobial substances produced by bacillus subtilis var . natto