细胞原位杂交

用细胞原位杂交方法，观察TGF－β1、TNF和维拉帕米对小鼠MφCD14mRNA表达的影响。

The peritoneal macrophage CD14 mRNA expression was observed by in situ hybridization analysis .

westernblot进一步检测I型胶原蛋白表达的动态变化；细胞原位杂交检测I型胶原基因表达。

Western blot was applied to further detect the process of collagen I protein expression , and in situ hybridization was used to examined the expression of collagen ⅰ gene .

方法以RNA真空转移法行NorthernBlot分析，以光敏生物素标记的探针行细胞原位杂交，对此两种细胞中p53mRNA转录水平分别进行比较研究。

METHODS The comparative studies on p53 mRNA transcriptional levels were conducted by analysis of Northern blot with vacuum transfer , in situ hybridization ( ISH ) with biotin-probes .

方法采用地高辛细胞原位杂交方法检测SD大鼠深Ⅱ度烫伤模型在伤后不同时段的创面渗出液细胞的bFGF和PDGF中mRNA表达变化。

With SD rat burn models , we detected the change of expression of bFGF and PDGF mRNA in the exuded cells from superficial and deep ⅱ 0 burn wounds by DIG in situ hybridization at different times .

孕早期绒毛细胞原位杂交监测人巨细胞病毒宫内感染的价值

Evaluation of Early Pregnancy Chorion Cells with in Situ Hybridization to Detect HCMV Intrauterine Infection

细胞原位杂交结果表明，休克及复苏后，小鼠腹腔巨噬细胞内CD14mRNA表达也明显增加。

The expression of CD14 mRNA in the peritoneal macrophages in mice was also enhanced after hemorrhagic shock and subsequent resuscitation showed by cell in situ hybridization .

臂旁核中的生长抑素mRNA阳性神经细胞&原位杂交组织化学研究

Somatostatin mRNA-containing neurons in the parabrachial nucleus of rats ── in situ hybridization histochemical study

此外，体外培养大鼠肝细胞，原位杂交法计数膜LDL受体阳性表达。

Besides , the hepatocyte of rat was cultured and analysis was made on the mRNA expression of low density lipoprotein receptor with hybridization in vitro .

用地高辛标记人巨细胞病毒（HCMV）DNA探针，对105例孕早期妇女（其中有异常妊娠史者45例）绒毛细胞进行原位杂交（ISH）。

The HCMV DNA probe labeled with digoxin was used for 105 women with early pregnancy ( including 45 cases with a history of abnormal pregnancy ) to make in situ hybridization ( ISH ) of chorion cells .

生物素标记核酸探针对间期细胞行原位杂交及临床应用

Interphase cell in situ hybridization with biotinylated nucleic acid probe and its clinical application

与此同时，在软脑膜、蛛网膜下腔、脑室及脑实质炎性病灶内，渗出的炎性细胞可见原位杂交阳性信号和免疫组化阳性染色颗粒，对照组未见炎性渗出。

Meanwhile , in the brain from the experiment rats , strong positive hybridization and immunoreactivity were observed in the infiltrated inflammatory cells in leptomeninges , subarachnoid cavity , ventricles and brain parenchyma .

方法用松胞素-B诱导的双核细胞荧光原位杂交技术，对人体内21号染色体非整倍体和培养细胞中21号染色体分离异常的发生率进行检测。

Methods Fluorescence in situ hybridization ( FISH ) with pericentric DNA probes specific to human chromosome 21 to the binucleated cells cytokinesis blocked by cytochalasin B ( CB ), namely , CB FISH method .

方法：应用21号染色体区域特异性探针对30例未经培养的早孕期绒毛细胞进行原位杂交，并同时行常规细胞遗传学分析以对比诊断。

Method : We used FISH with chromosome 21 specific probe on 30 uncultured chorionic villi cell samples to detect the Down 's fetus , and we also performed the conventional chromsome analysis of chorion cells from parallel samples .

不同脱钙方法对骨组织切片细胞内mRNA原位杂交表达效果的影响

Effect of expression results on mRNA in situ hybridization using different decalcification methods in rabbit bone growth plate cells

用免疫细胞化学和原位杂交技术探讨G、D细胞及胃泌素mRNA与肠化生的关系。

Using immunohistochemistry and in situ hybridization , we investigated the relationship between G , D cells , gastrin mRNA and intestinal metaplasia in human gastric mucosa .

采用传统的细胞遗传学和原位杂交技术相结合，检测了10例NK淋巴细胞瘤的淋巴结内瘤细胞。

Tumor cells of lymphoid node in 10 cases Nk cell lymphomas were detected by traditional cytogenetics and in situ hybridization ( ISH ) .

方法：应用组织块培养法培养成纤维细胞，用原位杂交检测胶原酶和TIMP-1的表达。

METHODS : Fibroblasts were cultured by tissue block culture method and the expression of MMP 1 and TIMP 1 was detected by in situ hybridization and quantitative analysis were used in this study .

方法收集和固定人滞留乳牙，用甲苯胺蓝和抗酒石酸酸性磷酸酶（TRAP）染色观察和定位破骨细胞，用原位杂交检测降钙素受体mRNA表达情况。

Methods After fixing the collected deciduous teeth , toluidine blue was performed and tartrate-resistant acid phosphatase ( TRAP ) staining was used to identify the osteoclasts on the resorbing surface of human deciduous teeth and in situ hybridization of calcitonin receptor mRNA to show its existence .

分别于光照前及光照后3h、12h、24h、72h终止培养，取细胞行Bcl-2mRNA原位杂交检测，观察细胞Bcl-2基因表达情况。

Bcl-2 mRNA in site hybridization ( ISH ) were detected in all cells before exposure to light , 3 hours , 12 hours , 24 hours and 72 hours after exposure and observed the expression of Bcl-2 gene .

孕早期滋养细胞的荧光原位杂交

Fluorescence in situ hybridization of trophoblast cells in first trimester of pregnancy

体外诱导分化神经细胞的原位分子杂交方法

In Situ Hybridization of RA Induced P19 Cells

方法将TGF-β1基因导入成骨细胞，通过原位杂交检测和成骨细胞培养上清液TGF-β1活性测定（水貂肺上皮细胞生长抑制试验），了解转染细胞TGF-β1的表达。

Methods The expression of TGF β 1 in the transfected osteoblasts was detected by in situ hybridization and assay of TGF β 1 activity in the supernatant ( mink lung epithelium cell growth inhibition test ) .

目的：探讨羊水细胞培养及荧光原位杂交（FISH）技术在产前诊断中的实验方法及应用价值。

Objective : To study the experimental method of amniotic cell culture with fluores-cence in situ hybrydization ( FISH ) for prenatal diagnosis and its application value .

常规细胞遗传学和荧光原位杂交技术在肺癌遗传学研究中的应用

Application of Conventional Cytogenetics and Fluorescence in Situ Hybridization in Lung Cancer

同一细胞非放射性双原位杂交信号显示技术

Demonstration of non-radioactive in situ hybridization with double signals in the single cell

离体细胞的非放射性原位杂交

Nonradioactive in Situ Hybrization of Cells In Vitro

以雌性小鼠为对照，用原位杂交法检测小鼠外周血涂片Y染色体阳性细胞；用荧光原位杂交法检测雄性小鼠脑组织切片Y染色体阳性细胞。

Compared with the female mice , the peripheral blood smear and brain tissue sections of the male mice were detected with in situ hybridization ( ISH ) and fluorescence in situ hybridization ( FISH ) .

结果得到抗G-418的阳性细胞克隆转染细胞，neo基因原位杂交阳性；

Results Cell clones with G - 418 resistance and positive signals of mRNA of neo gene were obtained .

用地高辛随机引物标记的人巨噬细胞炎性蛋白1α和血管细胞粘附分子1cDNA探针与各组内皮细胞进行核酸原位杂交。

The EC of all groups were hybridized , insitu , with the digoxigenin-labeled MIP-1 α and VCAM-1 cDNA probes .

方法采用染色体特殊位点的特异性探针（LSI21）对30例羊水细胞和6例绒毛细胞标本进行未培养间期细胞荧光原位杂交。

Methods : Uncultured interphase cells including 30 cases of amniotic fluid cell and 6 cases of villous tissue were performed FISH by using specific probe ( LSI21 ) of special site on chromosome .