质粒转染

方法研究主要应用Northern印迹杂交、Western印迹杂交、质粒转染技术以及细胞凋亡检测法，探讨Genistein抑制乳腺癌细胞生长的机制。

Methods Human breast cancer cell lines were treated in vitro with genistein . Northern blot , Western blot , plasmid transfection and apoptosis assay were used to evaluate the mechanism of cell growth inhibition .

D组：予空白质粒转染试剂瘤内注射，每日一次。

Group D : blank plasmid transfection reagent intratumoral injection once a day .

与单纯质粒转染组和阳离子聚合物转染组相比，光化学作用可明显提高阳离子聚合物介导转染目的DNA的阳性细胞率。

Photochemical effect could significantly increase the transfection efficiency of target DNA mediated by polycation .

质粒转染后亨廷顿蛋白过表达可显著增加溶酶体酶cathepsinB、D水平。

Over-expression of exogenous Htt in cells significantly increased levels of the lysosomal enzymes cathepsin B and D.

串珠素反义cDNA质粒转染对喉癌细胞Hep-2增殖能力的影响

Suppression of proliferation of Hep-2 cells by stable expression of anti-sense perlecan cDNA

转染后48h，MTT法示质粒转染组细胞的与对照组相比细胞增殖及存活受到抑制。

MTT assay indicated the growth of the plasmid transfected cells was inhibited .

RT-PCR产物电泳结果显示，与转染前细胞、空质粒转染细胞相比，转染survivin反义核酸的细胞SURVIVINMRNA水平明显降低。

As compared to controls , the level of survivin mRNA expression in transfected cells decreased significantly .

微囊化人类心房肽cDNA重组质粒转染细胞对实验性高血压大鼠肾组织学改变的影响

Effects of Encapsulated Plasmid Recombining with hANP cDNA Transfected Cells on Morphological and Histological Characteristic of Experimental Hypertensive Rats

方法重组质粒转染3T3细胞通过DNA磷酸钙共沉淀法；

Methods The recombinant plasmid was transfected into NIH / 3T3 by calcium phosphate DNA coprecipitation .

方法雌激素受体阴性的乳腺癌细胞株MDA－MB－231细胞，采用分子生物学质粒转染技术，将ER阴性的乳腺癌细胞MDA－MD－231转染成ER阳性细胞。

Methods The ER negative breast cancer cell line MDA MB 231 was transfected with the ER gene by stable transfection .

将重组质粒转染COS-7细胞，采用间接免疫荧光检测转染细胞S1蛋白的表达。

The expressed S1 protein were detected with indirect immunofluorescence assay .

westernblot和dotELISA分析显示，重组质粒转染细胞的裂解物中存在表达的Gag蛋白。

Western blot and Dot ELISA demonstrated that expressed Gag protein existed in the lysate of Hela cells transfected by recombinant plasmid .

方法：本课题前期阶段已将含谷氨酰胺酶基因C末端反义序列的质粒转染入胃癌细胞并命名为SGC-7901~（GA）。

Methods : The gastric carcinoma cells had been successfully transfected with plasmid containing the C'distal end antisense sequence of glutaminase gene and called SGC-7901GA in previous researches .

双调蛋白反义cDNA质粒转染及抗uPA抗体均导致乳腺癌细胞体外侵袭性的降低。

Amphiregulin cDNA antisense transfection as well as anti-uPA antibody treatment suppressed the invasive capability of tumor cell .

结论：串珠素反义cDNA质粒转染可以有效抑制喉癌细胞Hep-2的增殖能力。

Conclusion : The growth of Hep-2 cells could be inhibited significantly by perlecan anti-sense cDNA plasmids transfection .

然后将测序正确的质粒转染A549细胞，用westernblot检测转染效果，结果表明有很好的基因抑制效果。

And then sequencing the plasmid transfected A549 cells transfected with the Western Blot test results , results showed that the inhibitory effect of good genes .

构建好的重组质粒转染COS-7细胞，观察EGFP的表达情况。

COS-7 cells were transfected by recombinant plasmids to test the EGFP expression .

超声微泡介导EGFP质粒转染视网膜母细胞瘤细胞与传统转染方法效率对比

Experimental Research of Transfection Efficiency for EGFP Plasmid Transfected into Retinoblastoma Cells by Ultrasound Microbubble Intensifier

方法用带有GFP突变体的质粒转染到表达细胞，观察GFP瞬时表达的情况：1直接测定GFP在COS-7细胞中的表达并观察表达的稳定性；

Methods The plasmids carrying mutated GFP gene were transfected into the eukaryotic cells to observe transient gene expression .

结论：LipofectAMINE介导pBK－Signal－EGF质粒转染角朊细胞可获得瞬时表达的EGF蛋白。

Conclusion : The instantaneous expression of EGF was obtained after gene transfer with the PBK-Signal-EGF plasmid mediated by LipofectAMINE .

结论用SDF1特异性siRNA的表达质粒转染骨髓基质细胞，抑制了SDF1基因表达。

Conclusion The SDF-1 gene expression in human bone marrow stromal cells transfected with human SDF-1-specific siRNA expression plasmids is inhibited distinctly .

目的观测超声造影剂及超声辐照对TIMP-1siRNA质粒转染肝纤维化大鼠肝组织内TIMP-1基因表达的影响。

Objective To investigate the effect of ultrasound contrast agent and ultrasound irradiation on TIMP-1 siRNA expression in liver of hepatic fibrosis rats .

应用12质粒转染系统构建A/HNP/03（H9N2）重组禽流感病毒

Generation of a Reassortant A / HNP / 03 ( H_9N_2 ) Influenza a Virus by 12 Plasmid-based Transfection System

重组质粒转染BHK细胞，加入辅助病毒后，得到表达目的蛋白的重组AAV。

Recombinant AAV was obtained from the cell culture supernatant after adding helper virus .

将构建好的重组质粒转染COS-7细胞，检测荧光素酶的表达活性。

Then we transfected the recombinant plasmids into COS-7 cells and detected the expressional activity of luciferase .

利用磷酸钙法介导重组质粒转染COS-7细胞，westernblot方法鉴定外源蛋白在COS-7细胞中的表达情况，结果表明外源基因可以成功的在COS-7细胞中进行表达。

Western Blot was used to identify the expression of the protein and result showed that the interested gene was expressed successfully in COS-7 cells .

将此重组质粒转染COS-7细胞，通过RT-PCR及免疫荧光检测LACK基因在真核细胞中的表达。

This recombinant plasmid then was transfected into the eukaryotic cell COS 7 , and the expression of LACK gene in eukaryotic cell was detected by RT PCR and immunofluorescent staining .

结果：经鉴定所培养的细胞是NSC，采用脂质体转染方法成功的将pEBFP/FP6双表达质粒转染进NSC。

Results : Cultured NSCs were identified by immunohistochemistry . The pEBFP / FP6 plasmid was transfected into NSC by liposome .

与对照组相比，各重组质粒转染组细胞的克隆形成能力显著降低（P0.05），增殖活性被显著抑制（P0.05）。

Compared with the control groups , colony formation of the recombinant plasmid transfected cells was greatly decreased ( P0.05 ), the cell proliferation of the transfected cells was inhibited significantly ( P0.05 ) . 3 .

目的：利用HCV细胞感染模型和HCVCORE融合表达质粒转染细胞模型，探讨HCV病毒和CORE蛋白对miR-122表达水平的影响。

Objective : To explore the influence of HCV and core protein on the expression of miR-122 , using HCV cell culture model and HCV-core protein recombinant expression plasmid . Methods : 1 .