转染率
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此外,PEI也被认为具有高转染率和使内涵体破裂的能力。
Additionally , PEI is considered to exhibit high transfection efficiency and endosomal disrupting capacity .
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超声介导微泡造影剂对细胞和微血管以及基因在HUVEC内转染率的影响的研究
Study on Effects of Ultrasound-Mediated Microbubbles Contrast Agent on Cells , Capillaries and Transfection Efficiency of Gene in HUVEC
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脂质体介导外源性DNA阳性转染率可高达30%~40%以上。
Transfected rate of exogenous DNA mediated by lipofectin was above 30 % ~ 40 % .
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以NS组为对照并以GFP为荧光标记物,用流式细胞仪(flowCytometry,FCM)分析各实验组肝细胞的转染率。
TE of rat hepatocytes were analyzed by flow cytometry ( FCM ) using GFP as fluorescent marker .
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【结论】共聚物的结构组成对DNA复合物的理化性质、毒性和转染率都产生较大的影响。
[ Conclusion ] The copolymer block structure strongly influences not only the physicochemical properties of the DNA complexes , but also their cytotoxicity and transfection efficiency .
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TK的转染率可以通过应用改善载体来提高,如增殖型腺病毒。
The rate of TK transfection could be boosted by using proliferous adenovirus .
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外源TFPI基因的转染率为23%。
The transfection rate of exogenous TFPI gene is 23 % .
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结果经统计学分析显示,不同血清型AAV对三种细胞系转染率有显著差异。
RESULTS The transduction efficiency of AAV is distinct in the three lines .
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质粒带有eGFP,荧光显微镜观察和流式细胞仪计数确定转染率。
Because of the plasmid with eGFP , fluorescence microscopy and flow cytometry were used to determine transfection efficiency .
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结果:随着腺病毒感染复数量(multiplicityofinfection,MOI)增加,对Hep-2细胞转染率亦增加,100%转染所需的MOI为200。
Results : The more the multiplicity of infection ( MOI ), the more the adenovirus transfected efficiency to Hep 2 cells .
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目前有研究发现,超声微泡在超声作用下可明显提高基因的转染率,本研究利用超声微泡技术联合超声辐照,观察TFO对TF的作用效率。
Present study has indicated that ultrasonic microbubble under the action of ultrasound irradiation can improve gene transfection efficiency .
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结果:逆相蒸发法制备的脂质体包封率高于冻融&超声法(P<0.01)。流式结果显示两种方法制备的脂质体转染率无显著性差异(P>0.05)。
Result : The rate of liposome envelopment completed with the contra-phas-evaporated method is substantially ( P < 0.01 ) higher than the liposome completed with the thaw-freeze-ultrasonic method while the effect of transfection was of no sharp difference ( P > 0.05 ) .
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HSV-TK/GCV系统抗肿瘤效果不佳,或对某些肿瘤细胞无效的原因可能是TK转染率低和/或旁观者效应低。
The inefficacy of HSV-TK / GCV system against tumors would result in low TK gene transfection rate or / and bad BSE .
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FAM检测CEA转染率、成熟DC表型、CTL亚群比例、CTL体外杀伤率。
Detecting CEA transfection rate , the mature DC phenotype , the proportion of CTL subgroup , the killing rate of CTL in vitro with FCM . 3 .
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与缺乏FOL受体的A549细胞相比,由此而来的合成物在过量表达FOL受体的KB细胞中有更高的转染率。
The formulated complexes exhibited a considerably higher transfection efficiency against FOL receptor over-expressing KB cells than FOL receptor deficient A549 cells .
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脂质体介导的端粒酶反义寡核苷酸在人膀胱癌EJ细胞中的转染率及稳定性
In vitro transfection efficiency of telomerase antisense oligodeoxynucleotide mediated by liposome in human bladder cancer cell line EJ and its stability
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通过检测报告基因β-半乳糖苷酶(β-gal)的表达,发现最高转染率只在一较窄范围内获得。
A narrow range of conditions was founded to produce maximal level of transgene expression , as detected using the reporter molecule β - galactosidase (β - gal ) .
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用HEPES缓冲液能显著提高磷酸钙法转染率;
The transfection efficiency of calcium phosphate was greatly improved by using HEPES buffer ;
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若能降低此载体的LCST,使其在体温附近发生相转变,将有望通过调解温度进一步提高转染率。
It is expected that the transfection efficiency of PNI-AAM-G 1-3 could be further improved by changing its LCST below the body temperature .
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结论LPD是一种制备工艺简单、体外稳定性好、转染率高,具有应用潜力的非病毒载体系统。
Conclusion LPD could be prepared easily with small particle sizes and high transfection activities . LPD may be good non-viral vectors for applications in gene delivery .
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在本研究中得出:电压在350~450V之间,电穿孔法转染率与电压呈线性关系;
We found that the transfection efficiency of electroporation was linear with the voltage which was set from 350 to 450V ;
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48~72h阳性细胞明显增多,强度增强,高倍视野下转染率达20%~30%,多为明亮的绿色荧光的细胞。
From hour 48 to 72 the number of positive cells significantly increased , with strong intensity . The transfection efficiency was 20 % - 30 % under high-power field , and most of the cells were with green fluorescence .
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人牙髓细胞受Ad-hBMP-7感染48h后即可检测到外源蛋白表达;当感染复数为75时,重组腺病毒对牙髓细胞的转染率达(91.1±1.0)%;
Higher transfection efficiency ( 91.1 ± 1.0 ) % could be obtained at MOI of 75.Western blot from dental pulp cells infected with Ad-hBMP-7 for 48h detected protein expression of a hBMP-7 gene .
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以绿色荧光蛋白基因为报告基因,梭华Sofast转染率最高,约60%左右,而且在单个细胞中表达的GFP量多;
Using GFP as report gene , we found that the highest transfection efficiency can be acquired from Sofast among the four transfection reagents , it is about 60 % , moreover GFP expressed largely in single cell ;
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基因载体必须具备安全、稳定、转染率高等特点。
The carrier must be safe , stable , efficient .
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阳离子脂质体-DNA复合物与脂质-鱼精蛋白-DNA复合物体外细胞转染率比较
Transfection Efficiency Comparison of Cationic Liposome-DNA Complexes and Lipid-Protamine-DNA Complexes In Vitro
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并进行转染率的计算,探索最佳转染浓度及时间。
And calculate carrying rate to explore the best carrying concentration and time .
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最适感染复数为100,转染率可达90%以上。
When optimal MOI is 100 , transfection efficiency is above 90 % .
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超声造影剂介导下增强腺病毒相关病毒心肌靶向转染率的实验研究
The Study of Enhancement of Recombinant Adeno-associated Virus Transgene to Myocardium by Targeted Echo-contrast Agent
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但存在如何提高转染率,使细胞高效表达及生物安全性问题。
The problem lies in how to improve the transfection rate and target gene high expression .