阳性克隆

经过PCR鉴定确定了1个阳性克隆。

After the identification of PCR , one positive clone was obtained .

PCR鉴定阳性克隆，并外送测序。

Positive clone was analyzed by PCR , and sent to DNA sequencing subsequently .

目的：从DNA重组体中快速鉴定出阳性克隆。

Objective : To identify positive clones from DNA recombinants rapidly .

经VirtualNorthern杂交分析，获得6个阳性克隆。

Six positive clones were obtained by Virtual Northern blots ( Fig.3 ) .

通过反式Northern杂交筛选出3个阳性克隆进行测序。

By reverse Northern blot , 3 positive clones were obtained .

对酵母体内一对一验证的阳性克隆进行DNA测序。筛选的阳性重组表达质粒转化表达菌E。

Sequence the verified positive clones . The positive recombinant plasmids were made sequence .

通过PCR和DNA测序，挑选和鉴定阳性克隆。

Positive clones were selected and verified by PCR and DNA sequencing .

测定和分析7个杂交阳性克隆的DNA序列。

The DNA sequences of 7 selected clones with positive hybridization were determined and analysed .

阳性克隆经过PCR鉴定以及DNA测序鉴定后，进行慢病毒小量包装。

After PCR and sequencing identification , the positive clones were packaged into lentivirus .

一种快速筛选阳性克隆的方法&反向Northern印迹杂交技术

Fast screening positive clones with reverse northern blot

DNA测序结果显示，所得到的6个噬菌体阳性克隆中，有5个阳性克隆递呈相同的氨基酸序列。

DNA sequence assays showed that 5 of the 6 positive clones displayed the same peptide sequences .

结果文库扩增后得到28个阳性克隆，进行菌落PCR分析，均得到200～1000bp插入片段。

Results The amplified library contained 28 positive clones .

经PCR鉴定后，对阳性克隆进行测序；

Positive clones of HN gene were identified by PCR and then sequenced .

通过反向Northern斑点杂交法进一步筛选消减文库，对阳性克隆进行序列测定和同源性分析；

Reverse Northern dot blot was carried out to further screen the subtracted cDNA library .

结果从消减文库中随机挑取的100个白色克隆中筛选出13个阳性克隆，DNA测序获得11个不同的cDNA序列；

Results Thirteen positive clones were obtained , and 11 cDNA sequences were identified .

鉴定日本血吸虫cDNA阳性克隆三种方法的比较

Comparison of Three Methods of Identifying Schistosoma Japonicum Positive cDNA Clones

结果1个阳性克隆的插入子DNA全长867bp，前525b为编码区。

Results The insert of one positive clone contained 867 bp with the former 525 bp being coding region .

目的应用以菌落为模板的聚合酶链反应（PCR）技术筛选插有小鼠Doc1R基因组序列的重组阳性克隆。

Objective To screen the Doc 1R gene recombinant plasmid by use of colony PCR .

PCR扩增阳性克隆插入片段。

The inserts were amplified by PCR .

以特异性引物经基因组PCR及Southern印迹分析对阳性克隆进行检测。

Positive clones with the correct recombination were confirmed by genomic PCR and Southern blot analysis .

日本血吸虫童虫cDNA文库免疫学筛选及阳性克隆的初步鉴定

Immunoscreening and Identification of Schistosoma japonicum Juvenile cDNA Library

对克隆进行PCR鉴定，结果获得142个阳性克隆。

After identification and amplification by PCR with the T-vector primers , we isolated 142 positive clones .

克隆至大肠杆菌，用PCR鉴定及锥虫蓝培养基筛选阳性克隆；

Finally , positive clones were screened on trypan blue culture medium and expressed in DH5 α .

经质粒PCR及酶切鉴定后，对阳性克隆进行测序。

Positive clones of F gene were identified by PCR and restriction enzyme digestion and then sequenced .

结果：在没有任何筛选标记的情况下，用菌落PCR方法从10个菌落中成功挑选出1个重组阳性克隆。

Results : One positive clone out of ten clones was successfully identified without any selectable marker .

结果共筛选出13个阳性克隆，PCR扩增出特异的插入片段。

Results Thirteen positive clones were obtained after three rounds of immunoscreening , and all amplified by PCR .

人肝癌细胞cDNA文库的构建、筛选及阳性克隆分析

Construction and Screening of Human Hepatocarcinoma Cell cDNA Library and Analysis of the Positive Clones

目的：消减文库构建过程中，用PCR技术快速筛选重组阳性克隆。

Aim : To develop a PCR technique for rapid screening of recombinant plasmid in subtractive library of cDNA .

Neo基因的RTPCR法鉴定阳性克隆细胞；

The positive recombinants were identified by RT-PCR to amplify NEO genes .

利用噬菌体展示技术对突变库进行4轮富集筛淘及ELISA鉴定；以ELISA方法测定所获阳性克隆的特异性。

Hepatoma cell-specific scFvs were selected after four rounds of panning by phage display and ELISA detection .