倒置显微镜

A、B两组培养的细胞每天在倒置显微镜下观察细胞形态、生长特点；

The morphology and growth feature of conceal limbal epithelial cells in A and B group were observed by inverted microscope everyday .

方法：采用倒置显微镜下观察和台盼蓝染色、DNA琼脂糖凝胶电泳、MTT等方法，观察体外培养的H14被蛞蝓粗提物作用后的形态学及生化变化。

Methods : MTT method , DNA gel electrophoresis , cell stain and microscope observation .

结果：倒置显微镜观察，悬浮细胞培养法A组和组织块培养法B组扩增的角膜缘上皮细胞均能在羊膜上单层生长。

Results : A monolayer of cultured corneal limbal epithelial cells was observed by inverted microscope in both A group and B group .

诱导细胞形态通过倒置显微镜观察，并通过免疫细胞化学检测分化细胞表达Insulin和Nestin。

Differentiated cells were observed under inverse microscope , insulin and nestin expressed in differentiated cells were detected with immunocytochemistry .

在倒置显微镜下观察加药与未加药孔K562细胞形态。

K562 cells in test group and control group were observed by inverted microscope .

结构紧凑的扫描倒置显微镜THz成像系统

A Compact THz Imaging System Using Reverse-Microscope and Scanning Device

48h后，倒置显微镜下观察细胞形态变化。

After 48 hours morphologic change of cells was observed under invert microscope .

方法：采用全麻下抽取兔股骨骨髓，经体外培养的方法并在培养液中加入4种不同浓度的bFGF（0,60120240ng/ml）培养后经倒置显微镜观察；

Methods : The BMSC was cultured in vitro with different concentrations of bFGF ( 0,60,120,240 ng / ml ) .

多功能倒置显微镜下观察，计数粒巨噬细胞集落形成单位（CFU一GM）集落。

Colony Forming Unit of Granulocyte Macrophage ( CFU - GM ) were observed and counted under a multifunction inverted microscope .

24h后，倒置显微镜和透射电子显微镜下观察细胞的形态学变化。

Cells were incubated for 24h . Then we observed the morphological changes under optical microscope and transmission electron microscope .

夏枯草注射液（50mg/mL）作用于K562细胞后，倒置显微镜、姬姆萨染色、MTT染色光镜下观察，均可见典型的凋亡细胞的形态学特征。

( After ) K562 cells were treated with PVI ( 50 mg / mL ), the morphological characters of apoptosis were observed .

对人自体角膜缘干细胞进行体外培养，倒置显微镜观察培养细胞体外生长的形态，对培养出的细胞用抗角蛋白K3的单克隆抗体AE5进行鉴定。

The growth of cultured cells in vitro were observed under inverted microscope .

采用倒置显微镜观察细胞在DA膜片上的粘附和生长情况，并用MTT法测定细胞增殖率。

The morphology of cell adhesion on DA films was observed and photographed by invert light microscope . Using MTT method tested the cell growth rate .

12h后，用倒置显微镜观察细胞的形态学变化，流式细胞仪检测细胞凋亡率。

Morphological changes of cells were observed under the inverted microscope and apoptosis rate was tested by flow cytometry .

在倒置显微镜下，应用台盼兰染色法观察蟾酥氯仿提取物对T淋巴瘤细胞及Hela细胞的生长抑制作用。

Under the microscope , the inhibition effects of T-lymphoma cell and Hela cell treated by the compound were observed by TRYPAN-Blue assay .

倒置显微镜下观察NSCs形态及生物学特性，免疫细胞化学方法（SABC法）检测巢蛋白（Nestin）。

Observing the biological characteristics of NSCs under the inverted microscope morphological and immunocytochemistry ( SABC ) detect nestin .

细胞接种后3h在倒置显微镜下观察细胞生长情况，见细胞贴壁后每天观察2次。

Three hours later , the growth condition of cells was observed under inverted microscope , and twice daily after the cells attached .

结果倒置显微镜下，对照组RPE细胞在接种后第7天完全融合。

Results Under reversed microscope , RPE cells in control group were fused completely at the 7 ~ th day after inoculation .

用倒置显微镜观测细胞的形态学改变，分别用MTT、流式细胞术检测细胞的生长活性及细胞周期的改变。

Cellular morphology , cell proliferative activity and cell dividing cycle changes were observed by invert microscope , MTT assay and Flow Cytometry ( FCM ) assay respectively .

每日以倒置显微镜观察细胞形态及生长情况，MTT法绘制ADSC生长曲线。

Observe the cell morphology and growth by inverted microscope daily , MTT method to draw ADSC growth curve .

倒置显微镜和Giemsa染色观察到模型组肝细胞存在着明显的坏死和凋亡，小柴胡汤及柴胡&黄芩配方的凋亡细胞明显减少。

There were obvious necrosis and apoptosis in the model group cells observed under the convert microscope and Giemsa staining .

目的分析倒置显微镜计数、流式细胞仪及MTT等三种方法检测放射线照射后细胞存活率（S）结果间的差异。

Objective To analyze the difference among the cell survival probability ( S ) results , which were measured and calculated with microscope , flow cytometry and MTT methods respectively .

培养过程中的原代肝细胞，用MTT法检测细胞活力，绘制细胞生长曲线，并用倒置显微镜观察细胞形态。

The viability of cell was detected with MTT assay , cell growth curve was made on accordance with OD value , and cell morphology was observed in inverted microscope .

方法采用四甲基偶氮唑盐（MTT）比色法检测氯化三乙基锡对C6胶质瘤细胞的增殖抑制作用，采用普通倒置显微镜和HE染色形态学方法观察氯化三乙基锡对C6细胞增殖抑制作用。

Methods MTT colorimetry , optical microscope and HE coloration were used to examine the inhibitory effect of triethyltin chloride on the proliferation of C6 glioma cells .

方法将小鼠骨髓基质干细胞进行分离和纯化培养后，加入EGF和PDGF-BB，利用3H胸腺嘧啶核苷（3H-TdR）掺入反映细胞增殖效果，倒置显微镜下观察细胞增殖及分化状态。

Methods : Murine bone marrow stromal stem cells were isolated and cultured in vitro before EGF and PDGF-BB were added in .

方法：不同浓度苦参碱作用于体外培养AngⅡ作用的小鼠心肌成纤维细胞，利用倒置显微镜、PI/Hoechst33342双染结合荧光显微镜技术观察细胞形态；

Methods : Cardiac fibroblasts of mice were cultured and cell count , morphology of apoptosis cells were studied by fluorescence microscope with PI / Hoechst 33342 double staining .

选用不同浓度紫杉醇（1、5、10μg/ml）处理转染前后的胃癌细胞，四甲基偶氮唑蓝（MTT）比色法检测细胞增殖活性，倒置显微镜下观察细胞形态变化并摄影。

Gastric cancer cells were treated with different concentrations of paclitaxel ( 1,5,10 μ g / ml ) before and after transfection . Cell proliferation activity was detected by MTT colorimetric assay .

结果：1.用倒置显微镜观察发现青蒿鳖甲汤含药血清组、含药血清联合细胞因子组、细胞因子组BMSCs、DC的形态无差别。

With the inverted microscope , observed that the Qing Hao Bie Jia Soup containing serum group , a joint drug-containing serum cytokine , cytokine group of BMSCs , DC form of non-discriminatory . 2 .

通过倒置显微镜观察、HE染色、丫啶橙染色等手段研究PDLC的体外生长形态特征。为建立小鼠睾丸组织短期培养方法，对成年小鼠睾丸组织进行培养。

PDLC cultures were established by means of tissue explants method . Using the method of tissue culture mouse testes were cultured .

各组BT-325细胞分别进行以下检测：1.细胞形态学观察：倒置显微镜观察各组细胞的生长状况和形态上的改变；

Morphology observation of cell : Morphology of BT-325 cells were observed by invert microscope .