复制子

这样合成的每一片段DNA称为复制子。

Each segment of DNA thus synthesized is called a replicon .

HCV的新型体外细胞模型：复制子系统

Novel in vitro cell culture models for HCV : Replicon systems

来自邻近的复制子的新合成的DNA链又连结在一起。

Newly-synthesized DNA strands from adjacent replicons are ligated together .

基于甲病毒复制子载体的猪瘟DNA疫苗的免疫效力评价

Alphavirus Replicon - vectored Plasmid DNA - based Vaccine Elicits Protective Immunity Against Classical Swine Fever Virus

含RNA复制子表达载体介导的生长抑素基因在小鼠肌肉中的表达

Expression of SS gene in the muscle in mice by improved RNA-based replicon vector

RNA复制子疫苗研究进展

Research Development of RNA Replicon - based Vaccines

乙肝病毒RNA复制子疫苗、DNA疫苗的构建及其对小鼠免疫效果的比较

Construction of RNA Replicon Vaccine and DNA Vaccine of HBV and Comparison of Their Immune Efficiency in Mice

RNA复制子疫苗

RNA Replicon - based Vaccines

这些研究结果为选择性HCV全序列基因组复制子的研究奠定了基础。

These discoveries make it possible for the development of selectable full length genome replicon .

这些结果说明，DNA形式的西尼罗病毒复制子能够表达外源基因，具有良好的复制活性，为下一步观察该复制子的免疫原性奠定了基础。

All results demonstrated that the DNA-based WNV replicons could express exogenous genes and replicate itself well . This may set a foundation for following observation of replicon immunogenicity . 2 .

日本脑炎病毒（JEV）复制子表达载体的构建及其鉴定

Construction and Application of Subgenomic Replicon Vectors of Japanese Encephalitis Virus

基于RNA复制子GHRH基因表达载体的构建及在293细胞中的表达

The Construction of GHRH Gene Expression Vector Based on RNA Replicon and Its Expression in 293 Cell

基于DNA的Semliki森林病毒复制子载体体内外高水平表达外源基因

High-level Expression of Foreign Genes In vivo and In vitro by Improved DNA-Based Replicon Vector Derived From Semliki Forest Virus

基于SemlikiForest病毒RNA复制子的表达载体及其衍生的2型猪圆环病毒RNA疫苗的初步研究

Development of a Eukaryotic Expression Vector Derived from Semliki Forest Virus RNA Replicon and a Derivative RNA Vaccine Candidate Against Type 2 Porcine Circovirus Infections

RNA复制子疫苗克服了传统疫苗和普通DNA疫苗存在的缺点，具有抗原表达效率高、安全性好、应用范围广等优点，因而被视为一种发展前景很好的疫苗形式。

RNA replicon-based vaccine is regarded as a kind of promising vaccine because of its higher expression efficiency of antigen , higher security , wider range of application than classical vaccines and ordinary DNA vaccines .

目的本研究对原卟啉钠的制备方法进行了改进，以期简化其生产流程。并且体外观察原卟啉钠的细胞毒性作用及其对HCV亚基因复制子细胞模型RNA的抑制效果。

[ Objective ] To explore the improved preparation of protoporphyrin disodium , observe the cytotoxicity of Napp in vitro and its inhibitory effect on HCV subgenomic replicon cell model .

体外实验Napp对靶细胞安全作用范围较宽，且可有效抑制HCV复制子RNA。

Napp has little cytotoxic effect on target cells and it can effectively inhibit the RNA level of HCV subgenomic replicon cell model .

利用此蛋白上述两种功能特点，研究者构建了EBV复制子载体，这种载体已被广泛地应用于基因治疗。

On the basis of two functions of EBNA-1 , investigators have constructed successfully EBV-based plasmid , which can be used to gene therapy extensively .

传统DNA疫苗载体与Semliki森林病毒复制子对HIV-1Pr55~（gag）表达与体液免疫原性的比较性研究

Comparative Study of Expression and Humoral Immunogenicity of HIV-1 Group Specific Antigen Pr55 ~ ( gag ) by Conventional Plasmid Vectors Versus Semliki Forest Virus-Derived Vectors

XJ-160病毒复制子型表达载体的构建

Construction of Replicon Expression Vector Derived from XJ - 160 Virus

目的研究塞姆利基森林病毒（Semlikiforestvirus，SFV）衣壳蛋白5′翻译增强区对基于该病毒复制子的HIVGagDNA疫苗抗原表达水平和免疫原性的影响。

Objective To investigate the effects of 5 ′ translation enhancing region of Semliki forest virus ( SFV ) capsid protein gene on the gene expression and immunogenicity of HIV Gag DNA vaccine based on SFV replicon DNA vector .

如果在这种复制子中插入外源基因，由于复制子RNA在细胞胞浆内大量扩增，就使得外源基因得到有效表达，因此，复制子RNA可作为表达外源基因的良好载体。

When the heterologous genes are inserted into the replicon , amplification of replicon RNA in the cytoplasm of cells makes the heterologous genes express effectively , as a consequence , the replicon RNA can be made the excellent vector for expression of heterologous genes .

结果表明我们构建的XJ-160病毒复制子型表达载体具有自主复制功能，可以表达异源基因。

The results indicated that the replicon vector derived from XJ-160 virus was self-replicating and that the following gene expression was efficient .

目的利用塞姆利基森林病毒（SFV）复制子构建新型真核表达载体，并对含HIV-1中国流行株B亚型核心蛋白p24及多表位MEG嵌合基因的核酸疫苗进行表达与鉴定。

Objective To construct a novel eukaryotic expression vector based on Semliki forest virus ( SFV ) replicon and express the DNA vaccine containing subtype core protein p24 and multi-epitope MEG chimeric gene of epidemic strain B of HIV-1 in China .

结论SFVC蛋白翻译增强区能显著提高SFV复制子DNA疫苗的抗原表达和抗原特异性细胞免疫反应。

Conclusion Antigen expression and cellular immune responses induced by the HIV Gag DNA vaccine based on a SFV replicon DNA vector were significantly improved by fusing the translation enhancing region of SFV capsid gene to the 5 ′ terminus of gag gene .

丙型肝炎病毒非结构基因复制子模型的建立和应用

Subgenomic replicon model of hepatitis C virus and it 's application

基于日本脑炎病毒复制子载体系统的疫苗研究

Development of Vaccine Research Derived from JEV Replicon Expression Vector System

复制子系统性能评估模型的研究与实现

Research and Implementation of a Model for Evaluating Performance of Replication Sub-system

数据复制子系统的主要功能是完成异构数据的复制功能。

The function of data-copy subsystem is exchange data of different construction .

放线菌线型复制子新的生物学功能及其应用

Novel Functions of the Actinomycetales Linear Replicons and its Applications