外毒素

用C型肉毒梭菌经过滤除菌的外毒素作为杀鼠剂，并用浸泡法配制成各种浓度的毒素毒饵，系灭草原害鼠。

The rodenticide is exotoxin of botulinum model C , The botulin bait is made by immersion method .

PCR技术检测编码绿脓杆菌外毒素A基因

Detection of the Gene Encoding Exotoxin A of Pseudomonas Aeruginosa by PCR Technique

IL-2与绿脓杆菌外毒素A嵌合蛋白的研究

Preparation of IL-2 / Pseudomonas Exotoxin A Chimeric Protein

肉毒毒素是肉毒梭状杆菌产生的外毒素，有7种血清型（A～G），是目前已知的毒性最强的细菌蛋白质。

Clostridium botulinum may produce any of seven known serotypes of neurotoxin ( A-G ), which are the most toxic bacterial proteins known .

目的研究铜绿假单胞菌外毒素A（PE）的某些抗原性及其与辅助细胞的关系。

Objective Study on superantigen Pseudomonas aeruginosa exotoxin A ( PE ) and its relationship with accessory cell ( AC ) .

试验制备了抗绿脓杆菌外毒素－A血浆，使用马匹免疫抗原为精制PA－103菌株的外毒素－A。

Antitoxic sera was produced in horse with purified exotoxin A of Pseudomonas aeruginosa ( Strain PA 103 ) .

根据编码绿脓杆菌外毒素A基因设计引物，应用PCR技术检测绿脓杆菌外毒素A基因。

Primers were designed according to the encoding gene of Pseudomonas aeruginosa exotoxin A , and Pseudomonas aeruginosa exotoxin A gene was detected by PCR technique .

目的研究缓症链球菌外毒素蛋白的分子量、等电点、氨基酸组成及N末端序列。

Purpose To determine the molecular weight , isoelectric point ( pI ), amino acid compositions and N terminal amino acid sequence of streptococcus mitis pyrogenic exotoxin ( SMPE ) .

目的获得IL－2绿脓杆菌外毒素A嵌合蛋白，用于相关疾病的治疗。

Objective To prepare - 2 / Pseudomonas exotoxin A ( PEA ) chimeric protein used for the treatment of related diseases .

本文用间接ELISA法测定了本室研制的4株绿脓杆菌外毒素A单克隆抗体的相对亲和力。

In the paper , relative affinity of four monoclonal antibodies specific for Pseudomonas Aeruginosa Exotoxin A ( PEA ) were determined by indirect ELISA .

绿脓杆菌外毒素ArecA基因的融合及融合蛋白的表达

Construction and Expression of PEA recA Fusion Gene of P aeruginosa

目的：优化重组铜绿假单胞菌外毒素A（PEA）基因工程菌的发酵条件，实现PEA的高效表达。

Objective : To optimize the fermentable conditions of recombinant E.coli BL21 for high level expression of PEA .

结论耦联抗CD19的免疫脂质体可特异性地识别B淋巴瘤细胞，并作为良好载体将无毒绿脓杆菌外毒素单元Ⅲ带入细胞。

Conclusion Anti-CD19-immunoliposomes , as drug carriers , can specifically recognize B lymphoma cells and deliver non-toxic domain HI into the tumor cells .

金黄色葡萄球菌肠毒素（Staphylococcalenterotoxins，SE）是一系列由金黄色葡萄球菌分泌的外毒素。

The staphylococcal enterotoxins ( SE ) are a group of exotoxins produced by staphylococcal aureus .

利用体外细胞毒实验（MTT）方法，检测外毒素单元Ⅲ及靶向脂质体包裹的单元Ⅲ对肿瘤血管内皮细胞的杀伤作用。

MTT assay was used to detect the cytotoxicity of free domain ⅲ and domain ⅲ encapsulated in VEGF-liposomes against tumor-derived vascular endothelial cells .

重组白介素6-假单胞菌外毒素融合蛋白对正常BN大鼠骨髓粒系造血的作用

Effect of recombinant interleukin-6-pseudo - Monas exotoxin chimeric protein ( il-6-pe40 ) on in vitro granulopoiesis of normal BN rat

从绿脓杆菌、金黄色葡萄球菌、大肠杆菌、乳酸菌菌液中特异性地检出编码绿脓杆菌外毒素A基因。红茶菌液抑菌作用的研究coli混合菌液中，E。

The encoding gene of Pseudomonas aeruginosa exotoxin A could be specifically detected from Pseudomonas aeruginosa , Staphylococcus aureus , Escherichia coli and Lactobacillus by the PCR detection technology .

白喉毒素（diphtheriatoxinDT）是棒状白喉杆菌被茁噬菌体感染后分泌的一种外毒素。

Diphtheria toxin is an exotoxin secreted by Corynebacterium diphtheriae that has been lysogenized by β bacteriophage that carries the DT gene .

绿脓杆菌外毒素A与人组蛋白H3嵌合蛋白基因克隆及表达的研究

Study on the Cloning and Expression of Fusion Gene for Pseudomonas Exotoxin A and Human Histone H3

方法用PCR扩增白喉杆菌特异的白喉外毒素B基因（toxB）片段，琼脂糖凝胶电泳检测扩增产物。

Methods The specific fragment of diphtheria toxin B gene ( toxB ) was detected by PCR . PCR amplification products were examined by agarose gel electrophoresis .

综上所述，拟态弧菌HX－4菌株外毒素含有3种蛋白成份，相对分子量分别为154KD、143KD和117KD。

The results demonstrated that the exotoxin had three kinds of different protein whose molecular weights were 154 KD , 143 KD and 117 KD respectively .

为了观察重组绿脓杆菌外毒素A（PEA）受体结合区蛋白在不同动物体内的免疫效果，将该蛋白纯化后免疫了小鼠、家兔、山羊等动物。

For observing the immune result of the recombinant receptor binding protein of the exotoxin A of P.aeruginosa in different animals , the mice , rabbits and goats were immunized with the purified protein , then challenged with PEA .

肉毒神经毒素（BoNT）是厌氧梭菌产生的蛋白类外毒素，有A～G7个血清型。

Botulinum neurotoxin ( BoNT ), which produced by anaerobic bacterium Clostridium botulinum , is a protein toxin that causes the most severe form of food poisoning , called botulism .

用逐步稀释透析法将纯化的大肠杆菌表达的重组绿脓杆菌外毒素A（PEA）受体结合区蛋白初步复性，复性后的可溶性蛋白用于竞争抑制PEA的细胞毒性实验。

The purified recombinant receptor binding protein of exotoxin A of P aeruginosa expressed in E coli was renatured preliminarily by diluted progressively dialysis . The renatured soluble protein was used in the experiment of competitive inhibition for cytotoxicity of PEA .

虽然App的毒力因子是多冈素的，其溶血外毒素（Apx）被认为是该菌最主要的毒力因子及保护性抗原，其N-端具有保护性抗原表位。

The virulence of App is multifactorial , but Apx is considered not only the most important virulence factor but also the major protective antigen in App . The protective epitopes might be located at the N-terminal fragment of Apx .

目的建立稳定、高效表达重组铜绿假单胞菌外毒素A（rEPA）的工程菌发酵及表达产物纯化工艺。

Objective To develop a procedure for fermentation of engineering E. coli strain stably and highly expressing recombinant Pseudomonas aeruginosa exotoxin A ( rEPA ) and for purification of expressed product .

目的探讨重组人促黄体激素释放激素-绿脓杆菌外毒素A（LHRH-PE40）在胃癌细胞及正常细胞是否存在相应受体表达及存在竞争性抑制。

Objective To study whether luteinizing hormone-releasing hormone aeruginosa exotoxin 40 ( LHRH-PE40 ) can be expressed in the gastric carcinoma cells or the normal cells and detect the competitive inhibition .

将绿脓杆菌外毒素、2A序列、IL2受体穿膜区以及新霉素磷酸转移酶的基因依次融合构建新型报告基因&peo，该报告基因能够特异性地甄别具有信号肽编码序列的基因。

A new reporter gene named peo was obtained by in turn fusing Exotoxin A of Pseudomonas aeruginosa , 2 A sequence , transmembrane region of IL-2 receptor and neomycin phosphotransferase genes , Peo is able to report genes encoding secretory proteins specifically .

Furin底物广泛，包括大多数的肽类激素、许多生长因子、受体、黏附分子、基质金属蛋白酶和某些病毒包装糖蛋白以及细菌外毒素，因此具有重要的生物学功能，并与许多疾病的发生发展有密切的关系。

Having a wide variety of substrates , including most peptide hormones , growth factors , receptors , adhesion molecule , matrix metalloproteinase , some virus envelope glycoprotein and bacterial exotoxins , Furin possesses important biological functions and bears a close relationship with the development of many diseases .

结果成功扩增出PLO溶血素基因，大小为270bp；试验结果表明，山羊化脓隐秘杆菌SC-1株对小鼠具有致死性，外毒素是其主要致病因子，内毒素无致病性或致病力不强，具有PLO溶血素基因。

The PCR products PLO hemolysin gene , the size of 270bp ; test results showed that the Arcanobacterium pyogenes SC-1 in mice with lethal and external toxins is the main pathogenic factor , endotoxin induced non-pathogenic or disease was not strong , with the PLO hemolysin gene .