异柠檬酸

结核杆菌H（37）Rv异柠檬酸裂解酶基因的克隆及表达

Clone and Expression of Isocitrate Lyase Gene in Mycobacterium tuberculosis H_ ( 37 ) Rv

苹果酸脱氢酶及异柠檬酸脱氢酶扩增对E.coli产琥珀酸的影响

Effect of Malate Dehydrogenase and Isocitrate Dehydrogenase Amplification on Succinic Acid Production in Escherichia Coli

由异柠檬酸氧化而来，同时伴有NAD的还原。

It is synthesized by oxidation of isocitrate , coupled to the reduction of NAD .

目的通过研究马尔尼菲青霉（PM）异柠檬酸裂解酶（ICL）基因转录水平与其酵母相生长的关系，初步探讨乙醛酸循环在PM致病中的意义。

Objective To study the relationship between transcriptional level of isocitrate lyase and development of Penicillium marneffei yeast and to investigate the significance of glyoxylate pathway in the pathogenesis of P.marneffei .

为了测定异柠檬酸裂解酶的活性，需要实时测定NADH量的减少。异柠檬酸在异柠檬酸裂解酶催化下形成乙醛酸，乙醛酸可通过乳酸脱氢酶和NADH还原成甘醇酸酯。

To determine the activity of the isocitrate lyase , the glyoxylate formed during catalysis was reduced to glycolate by lactate dehydrogenase with the concomitant oxidation of NADH .

异柠檬酸脱氢酶（IsocitrateDehydrogenase，IDH）是三羧酸循环的关键性酶，为新陈代谢提供能量和生物合成前体物质。

Isocitrate dehydrogenase ( IDH ) is one of the key enzymes in the TCA cycle , which involves in providing energy and biosynthetic precursors for metabolism .

利用淀粉凝胶电泳技术，对外源DNA直接导入栽培大豆的变异后代，进行了磷酸己糖异构化酶（GPI）、异柠檬酸脱氢酶（IDH）和葡糖磷酸变位酶（PGM）的同工酶酶谱分析。

With the method of starch Gel Electrophoresis , analysis of patterns of GPI , IDH and PGM were made in variant progenies of soybean cultivars which exogenous DNA was introduced directly .

纯化的目的蛋白异柠檬酸裂解酶的酶活性为16.7u/mg。

The enzyme activity of the purified protein was 16.7u/mg .

我们参考Bell法，以135名健康人作对照，测定了165例肝病和20例非肝病患者血清异柠檬酸脱氢酶（ICD）和传统项目SGPT的活性。

Referring to Bell 's method , we have determined the activity of isocitrate dehydrogenase , ( ICD ) and SGPT of traditional items of 165 cases with hepatopathy and 20 non-hepatopathy compared with 135 health .

预测NADP-IDH存在一个异柠檬酸脱氢酶超家族的保守结构域。

The existence of a conservative domain of isocitrate dehydrogenase superfamily of NADP-IDH was predicted .

结果表明，NCS明显抑制光下培养的离体萝卜子叶叶绿素含量及鲜重增加，对异柠檬酸裂解酶及羟基丙酮酸还原酶活性也显示出明显的抑制作用。

The results indicated that NCS markedly inhibited the increase of chlorophyll content and the fresh weight of excised radish cotyledons during a period of growth in light . The isocitrate lyase and hydroxypyruvate reductase activities were also significantly inhibited by NCS .

结果表明：（1）在生长期随着体重的增大，肌肉组织中脂肪合成酶异柠檬酸脱氢酶（ICDH）活性显著高于苹果酸脱氢酶（MDH）活性（P<0.01）；

Results showed as follows : ( 1 ) With gaining of body weight , the activity of ICDH was significantly higher than that of MDH in muscle ( P < 0.01 ) .

添加半胱胺能显著提高皮下脂肪激素敏感脂酶（HSL）活力38.23%（P<0.05），有降低苹果酸脱氢酶、葡萄糖6磷酸脱氢酶、异柠檬酸脱氢酶活性的趋势。

The results also showed the activity of hormone sensitive lipase ( HSL ) was increased by 38.23 % ( P < 0.05 ) . Compared with the control , the activities of malic dehydrogenase , glucose-6-phosphate dehydrogenase and isocitric dehydrogenase had a decreasing trend .

血清异柠檬酸脱氢酶测定及其临床意义

The Determination and Clinical Value of Serum of Isocitrate Dehydrogenase

基于结构分枝杆菌异柠檬酸裂合酶的高通量药物筛选模型的构建与应用

Construction and Application of Mycobacterium Tuberculosis Isocitrate Lyase-based High-throughput Drug Screening Model

酵母双杂交筛选结核分枝杆菌异柠檬酸裂合酶的相互作用蛋白

Identification the Interacting Partner of Mycobacterium Tuberculosis Isocitrate Lyase by Yeast Two-hybrid

马尔尼菲青霉异柠檬酸裂解酶的表达与其酵母相生长的关系

The relationship between expression of isocitrate lyase and development of penicillium marneffei yeast

经异柠檬酸节点分析发现，突变株合成谷氨酸的流量增加。

The flux to glutamic acid from the node of isocitrate was increased .

NAD~+-依赖型异柠檬酸脱氢酶的结构和功能研究进展

Advance on structure and function of NAD ~ + - dependent isocitrate dehydrogenase

异柠檬酸裂解酶&抗结核药物的新靶点

Isocitrate Lyase-a new target in TB drug research

异柠檬酸脱氢酶，兔抗猪，生物素。

Isocitrate Dehydrogenase , Rabbit anti-Swine , Biotin .

种子中异柠檬酸裂解酶活性的测定

Determination of Isocitrate lyase Activity in Seeds

植物NADP~+-依赖型异柠檬酸脱氢酶的分子进化及功能研究进展

Advances in molecular evolution and functions of NADP ~ + - dependent isocitrate dehydrogenase in higher plants

赤霉酸对小麦和大麦糊粉层细胞中异柠檬酸裂解酶形成的作用

The effect of gibberellic acid on the formation of isocitrate lyase in the aleurone cells of wheat and barley

伴随着种子的发育进程，6－磷酸葡萄糖脱氢酶、异柠檬酸裂解酶、异柠檬酸脱氢酶和琥珀酸脱氢酶的活性均有不同程度的增强。

There were gradually increased in activities of glucose-6-phosphate dehydrogenase , isocitrate lyase , isocitrate dehydrogenase and succinate dehydrogenase .

结核分枝杆菌异柠檬酸裂解酶基因的克隆表达及酶学性质研究

Molecular cloning , expression , purification and properties of isocitrate lyase gene from Mycobacterium tuberculosis H_ ( 37 ) Rv

其中，酶学水平上，比较了两菌株在初级代谢过程中的四种关键酶酶活，分别是葡萄糖-6-磷酸脱氢酶、柠檬酸合成酶、异柠檬酸裂解酶和苹果酸合成酶。

Glucose-6-phosphate dehydrogenase , citrate synthase , isocitrate lyase and malate synthetase are the four key enzymes involved in the primary metabolism .

异柠檬酸裂解酶、α-和β-淀粉酶的活性先上升，然后下降；

In the meantime , the activities of α - and β - amylase and isocitrate lyase in seeds rose initially , and then decreased .

乙醛酸循环关键酶，异柠檬酸裂解酶和苹果酸合成酶分别为正常尿素培养物的2.6和2.8倍。

The activities of the key enzymes of the glyoxylic acid pathway , isocitrate lyase and malate synthetase are 2.6 and 2.8 times of those the control culture .

在油分旺盛合成期，6－磷酸葡萄糖脱氢酶和异柠檬酸裂解酶的活性均达到了最大值，而此时，异柠檬酸脱氢酶和琥珀酸脱氢酶的活性均增加较慢；

The activities of glucose-6-phosphate dehydrogenase and isocitrate lyase increased to maximum , however , the activities of isocitrate dehydrogenase and succinate dehydrogenase increased slowly when oil synthesized rapidly .