抗体技术

单克隆抗体技术（monoclonalantibodytechnique）又称杂交瘤技术，是20世纪70至80年代诞生的生物高新技术。

The monoclonal antibody technology ( monoclonal antibody technique ) is also called the hybridoma technology , and it is a high and new biological technology which was borned in the 70s and 80s of 20th century .

流式细胞仪（FlowCytoMeter，FCM）是一项集激光技术、电子物理技术、光电测量技术、计算机技术以及细胞荧光化学技术、单克隆抗体技术为一体的新型高科技仪器。

Flow cytometer ( FCM ) is a collection of electronic and physical technology , photoelectric measuring laser technology , computer technology and the cell fluorescence chemical technology , monoclonal antibody technology as a new type of high-tech instruments .

一种敏感、快速、特异的抗双股DNA测定法&短膜虫酶标记抗体技术

A Sensitive , Rapid and Specific Assay for Double Stranded DNA Antibodies

本文应用单克隆抗体技术，检测了30例心血管疾病病人的外周血B细胞、T细胞及其亚群。

The peripheral blood B and T lymphocyte subsets of 30 patients with primary hypertension and coronary heart disease were determined by utilizing McAbs .

群体反应性抗体技术及HLA配型在1700例肾移植中的应用研究

Panel reactive antibody screening and human leukocyte antigen typing in 1700 cases of renal transplantation

SPA固化抗体技术及其在放射免疫分析中的应用

Immobilization of antibody via SPA with potential use in radioimmunoassay

在治疗后不同天数通过活检标本的细胞DNA周期分析、创面愈合的电脑图像积分和用抗角化蛋白荧光抗体技术等方法观察了上皮愈合的情况。

Wound healing was evaluated by its healing rate quantitated by computed image analysis , DNA cell cycle analysis using flow cytometry and the degree of reepithelialization quantitated by anti-keratin antibodies .

单克隆抗体技术分泌抗细粒棘球蚴囊液抗原McAb杂交瘤细胞株的建立

Establishment of hybridoma cell line secreting monoclonal antibodies ( McAb ) against hydatid cyst fluid antigen

乳过氧化物酶法制备~（125）Ⅰ标记膀胱癌（L4B4）单克隆抗体技术

Preparation of ~ ( 125 ) i-labeled monoclonal antibody of bladder neoplasm using lactoperoxidase

经与间接荧光抗体技术（IFAT）比较，两种方法检出抗体阳性和阴性的一致率为96.77%。

Compared HIA with IFAT , consistent rate of results was 96.77 % .

方法：1.免疫细胞化学方法和免疫荧光抗体技术检测成熟DC胆碱能系统（nAChRα7、ChAT和AChE）蛋白的定位表达。

Fixing expression on protein of cholinergic system ( nAChR α 7 , ChAT and AChE ) were measured with immuocytochemistry and immunofluorescence methods . 2 .

方法分别应用放射免疫分析和单克隆抗体技术对31例癫痫患者进行了血清IL-2、IL-6、TNF-α含量及外周血B细胞及T淋巴细胞亚群水平的测定，并与35例正常人作比较。

Methods Serum IL-2 , IL-6 and TNF - α levels were determined with RIA and peripheral lymphocyte examination were performed with mono-clonal antibody technic in 31 patients with epilepsy and 35 controls .

方法应用碱性磷酸酶标记抗体技术，观察CD44在胚胎肾组织和肾小球疾病患儿肾活检组织中的表达。

Methods The expression of CD44 in fetal kidneys and renal biopsy material from patients with glomerular diseases was observed by using alkaline phosphatase labelled antibody technique .

方法：利用流式细胞仪和单克隆荧光抗体技术测定31例BD患者和27例健康对照者外周血中CD4+CD25+调节性T细胞在CD3+CD4+细胞中的比例。

Methods : The proportion of peripheral blood CD4 + CD25 + Tr was determined by flow cytometry in 31 patients with BD and 27 healthy controls .

采用一步法单克隆抗体技术和微量序列特异性引物聚合酶链反应（Micro-PCR-SSP）技术进行HLAⅠ类和Ⅱ类分型。

Donor and recipients HLA class ⅰ and ⅱ typing was performed by one-step monoclonal antibody and micro polymerase chain reaction with sequence-specific primers ( Micro-PCR-SSP ) .

方法应用VIDAS检测CHL与细胞培养比较，不符者使用直接荧光抗体技术验证；

Methods C. trachomatis in urogenital samples was detected by both cell culture and VIDAS CHL . The different results were confirmed by direct fluorescent antibody assay ( DFA ) .

通过单克隆抗体技术对IBV抗原表位的研究，不但可以深入了解IBV的抗原结构，而且便于设计科学合理的表位疫苗和诊断试剂，对IB的诊治具有重要的意义。

IBV epitope studies by monoclonal antibody technology , not only help to understand the IBV antigenic structure in depth , but also facilitate to design scientific and rational epitope vaccines and diagnostic reagents .

随着分子生物学的进一步发展，抗体技术也逐步由细胞工程抗体向基因工程抗体演替，特别是单链抗体（ScFv）在理论和应用方面的研究令人尤为关注。

With the developing of molecular biology , Antibody technology has a succession from cell engineered antibody to genetically engineered antibody , especially the study of single-chain variable fragment ( ScFv ) is concerned in theory and application .

在分析二氟沙星（Difloxacin，DIF）分子结构和免疫原性基础上，运用单克隆抗体技术制备其单抗，并组装了竞争ELISA试剂盒和胶体金试纸。

In the study , Based on the analysis of molecular structure and immunogenicity of Difloxacin , the technology of monoclonal antibodies production was applied to assemble rapid ELISA kit and strip for DIF residual detection .

方法应用流式细胞仪（FCM），荧光素标记单克隆抗体技术测定68例2型糖尿病患者（血管病变35例，无血管病变33例）及31例正常人CD62P、CD63的阳性表达率。

Methods 68 type 2 diabetes mellitus ( 35 cases with angiopathy , 33 cases without angiopathy ) and 31 normal individuals entered this study . The expression level of CD62P and CD63 were measured by flow cytometric immunoflurescence .

目的：利用单克隆抗体技术检测闭角型青光眼（ACG）患者外周血T细胞亚群，以了解ACG患者机体的整体免疫平衡状态。

· AIM : To understand the whole immunologic balance state of the body of angle-closure glaucoma ( ACG ) patient by testing T cell subset in the peripheral blood of ACG patient with monoclonal antibody technique .

方法：分别应用单克隆抗体技术和酶联吸附试验检测31例白血病患者外周血T淋巴细胞亚群和sIL2R水平，并以35名正常人作对照。

Methods : Serum levels of sIL 2R and T lymphocyte subgroup in 31 leukemia patients and 35 healthy persons as control were determined by Elisa and monoclone of antibody technique .

荧光抗体技术检测病毒活疫苗中衣原体的污染

Detection the Contamination of Chlamydia in Live Vaccine with Fluorescent Antibodies

结论用基因工程抗体技术制备抗体是可行的。

CONCLUSION Preparing antibodies by genetic engineered antibody technology is feasible .

方法间接荧光标记抗体技术检测广州管圆线虫抗体，间接证实广州管圆线虫感染。

Methods detection of serum antibodies by indirect fluorescent antibody test .

单克隆抗体技术皮肤浆细胞增多症1例

Monoclonal Antibody Technique Cutaneous plasmacytosis : A case report

狂犬病病毒快速荧光抗体技术测定的研究

A rapid fluorescent antibody technique for monitoring rabies virus

单克隆抗体技术的发展&基因工程抗体

Development of monoclonal antibody technology & genetic engineering antibody

免疫珠试验检测抗精子抗体技术在体外受精-胚胎移植中的应用

Application of immunobead test in vitro fertilization-embryo transfer

抗体技术由细胞工程抗体（杂交瘤-单克隆抗体）发展到基因工程抗体，尤其是抗体库技术的出现，将抗体工程发展到了一个新阶段。

Antibody technology has been developed from cellular engineering antibody to genic engineering antibody .