梨火疫病

免疫吸附PCR技术提高梨火疫病菌检测灵敏度的研究

Sensitivity enhancement by using of immuno - capture - PCR for detecting of Erwinia amylovora

本研究首次从梨火疫病菌中克隆sdiA基因并作为新靶标应用于该病菌分子检测。

Amylovora and as a new target applied for molecular detection of Erwinia amylovora .

以梨火疫病细菌基因组DNA为模板，通过合成5′－端及3′－端一对特异引物，利用多聚酶链式反应（PCR）扩增获得harpin蛋白基因。

The entire coding sequence of harpin protein gene was amplified by means of PCR using genomic DNA of Erwinia amylovora as template and a pair of specific oligonucleotides at 5 ′ and 3 ′ end as primers , and cloned into E.

以梨火疫病随进境苹果果实传入的可能性为例，利用Monte-Carlo模拟方法评估有害生物的入侵风险。

Monte-Carlo simulation was used for the evaluation of the risk probabilities of invasion of the fire blight disease caused by Erwinia amylovora via imported apple fruits by considering that the proportion of infected fruits varying on the production area and the year .

利用本实验室自行设计的梨火疫病菌特异性引物REA-FEA，再次验证了免疫吸附-PCR技术的灵敏性和专化性，得到了同样的结果。

Using the specific primers REA-FEA designed in this work by ourselves , we proved the specificity and sensitivity of the immuno-capture-PCR method again . The same result has been achieved .

梨火疫病菌在中国的潜在分布及入侵风险分析

Potential Distribution of Erwinia amylovora in China and Invasion Risk Assessment

梨火疫病菌群体感应系统的初步研究他把那个梨平分给这两个小孩。

A Primary Study of Quorum Sensing in Erwinia Amylovora ; He halved the pear between the two children .

【结论】梨火疫病在中国的大部分地区适生，在有适宜寄主分布的地区，就可能严重发生。

【 Conclusion 】 Fire blight can survive in the majority part in China and might blast under suitable host plants and environmental conditions .

结果表明，这2种方法可以准确、灵敏地检测梨火疫病菌及样品，方法简单、快速、实用，可以在中国口岸推广使用，减少梨火疫病进入中国的风险。

It shows that these two methods were specific and sensitive , could be used to detect this disease in ports of China , and could reduce the risk of fire blight entry to China .