溶解酶

枯草杆菌SBS液体发酵联产血栓溶解酶和γ-聚谷氨酸

Co-production of thrombolytic enzyme and γ - polyglutamic acid by liquid-culture of Bacillus subtilis SBS

目的：本文研究尖吻蝮蛇毒纤维蛋白溶解酶FⅡa体内的溶栓作用。

AIM : A fibrinolytic enzyme from Agkistrodon acutus venom , called F ⅱ a , was tested for thrombolytic activity in animals .

基质金属蛋白酶（MMPs）是一组能降解所有细胞外基质的蛋白溶解酶家族，亦是致类风湿性关节炎（RA）不可逆关节损害的主要原因之一。

Matrix metalloproteinases ( MMPs ) are a family of matrix-degrading enzymes of the extracellular matrix and contribute to inreversible joint destruction .

兔眼玻璃体腔内注射1U纤维蛋白溶解酶7天内只能诱发出部分性PVD87.5%（7/8）。

The eyes of group B could induce partial PVD 87 . 5 % ( 7 / 8 ) .

在家兔连续多次给药实验中，测定给药20min时纤维蛋白溶解酶活性显著增加（P<001），凡给药24h后测定，纤维蛋白溶解酶活性均不见明显增强。

In the experiment on rabbits , in which they were given injections several times continuously , the activity increases markedly 20 minutes after the application ( P < 0.01 ), but there is none 24 hours later .

将交联纤维蛋白胶体置于pH7.6的TBS缓冲液中，加入纤维蛋白溶解酶。经过37℃摇床水解48小时，交联纤维蛋白胶体基本完全降解为可溶性降解产物。

The cross-linked fibrin gel was placed into pH7.6 TBS buffer , adding fibrinolytic enzymes , after shaking at 37 degrees Celsius for 48 hours , cross-linked fibrin almost completely degraded into soluble degradation products .

0.1U软骨素酶联合0.5U或1.0U纤维蛋白溶解酶眼内注射可以在短期内产生玻璃体后脱离，但在1周内产生的玻璃体后脱离为部分性。

Posterior vitreous detachment can induced by 0.1 U chondroitinase ABC combined with 0.5U or 1.0U plasmin in 1 week in rabbit eye , but the PVD is partly in 1 week .

长龙通注射液对家兔、豚鼠能增加纤维蛋白溶解酶活性，给药后20min、50min时最显著，90min后作用明显减弱或恢复正常。

This injection is capable of increasing the activity of fibrinolysin in rabbits and cavies , and the best result is seen 20 and 50 minutes after the application , but its effect markedly lessens or gets back to normal 90 minutes after the application .

蚯蚓纤维蛋白溶解酶的提取分离纯化

A Study on Extraction and Separation and Purification of Earthworm Fibrinolysin

尖吻蝮蛇毒无出血活性纤维蛋白溶解酶的纯化及其理化性质研究

Purification and characterization of non-hemorrhagic fibrinolytic enzyme from Agkistrodon acutus

纤维蛋白溶解酶是溶解血纤维蛋白凝块的药物。

Fibrinolytic enzymes are agents that dissolve fibrin clots .

总氧化剂和细胞溶解酶都正常。

Tox and vitreous lytes are all normal .

蛇毒纤维蛋白（原）溶解酶的研究进展

Progress in the research of the fihrino ( geno ) lytic enzymes from snake venoms

血纤维蛋白溶解酶是一类能直接降解血纤维蛋白的酶，在体外其主要来源于微生物、蚯蚓、蛇毒、某些海洋无脊椎动物和海藻等。

The fibrinolytic enzyme that directly hydrolysis fibrin mainly originate from earthworm , snake venom and microorganism .

胶原蛋白溶解酶注射治疗腰椎间盘突出症后症状加重再次治疗31例的临床观察

Clinical Observation on 31 Cases by Treating again which Sympton Increased after Collagenase Injection for Lumbar Disc Chemonucleolysis

纳豆激酶是一种纤维蛋白溶解酶，有望开发成为新型的溶栓药物。

Nattokinase ( NK ) is a kind of fibrinolytic enzyme , which is expected to be a new thrombolytic drug .

本文报告湖南尖吻腹蛇纤维蛋白溶解酶（简称蛇纤溶酶）对23例单纯高粘血症和31例脑血栓形成患者血液流变学的影响。

The paper reported the effect of fibrinolysin from Hunan Agkistrodon acutus on hemorheology in23 cases of hyperviscosaemia and31 cases of cerebral thrombosis .

用牛血纤溶酶原Separose4B亲和层析方法从蚯蚓粗提物丙酮粉中分离纯化一种纤维蛋白溶解酶。

A fibrinolytic enzyme was isolated and purified from the crude extract of the earthworm by using affinity chromatography on bovine plasminogen sepharose 4B column .

血凝块形成是一个缓慢但正常的生理过程，对抗不希望的血凝块的天然保护者是纤维蛋白溶解酶系统。

Formation of the blood clot is a slow but normal physiological process , and the nature 's guard against blood clots is the fibrinolytic enzyme system .

细胞外基质和基底膜的主要组织结构为胶原、层粘素和纤维结合素等，这些结构的破坏与降解需要相应的溶解酶参与。

The main tissue structures of basement membrane and extracellular matrix including collagen , laminin and fibronectin . To decompose and deconstruct these structure need the effect of resolvase .

利用纤维蛋白溶解酶（纤溶酶）溶解血栓，使血管再通是目前防治此类疾病的有效手段。

Using the fibrinolytic enzyme to dissolve the thrombus and cause blood vessel to be unblocked is the effective method to prevent and control this kind of disease at present .

目的：通过纤维蛋白溶解酶（以下简称纤溶酶）和透明质酸酶兔玻璃体腔内注射，研究玻璃体视网膜超微结构改变，阐明玻璃体后脱离的发生机制。

Objective : Through vitreous injection of plasmin or hyaluronidase , investigate the ultrastructural change of the vitreoretinal interface , and illuminate the mechanism of posterior vitreous detachment ( PVD ) .

结果肿瘤血管生成主要与生长因子、溶解酶及炎症细胞特异性分子表达增加有关。研究证明许多天然或人工合成化学分子通过作用于上述血管增生反应中的特异性分子发挥抗肿瘤效应。

RESULTS Growth factors , lytic enzymes and inflammatory cells were main molecular mediators in cancer angiogenesis and were proved to be target of many natural or synthetic chemically molecules in cancer therapy .

侵袭性是恶性肿瘤的重要生物学特征，侵袭性高的肿瘤细胞在分泌各种溶解酶破坏细胞外基质的同时，还可通过增强的迁移能力使其更容易向周围组织浸润。

Tumor cells of high invasion secret various dissolved enzymes which destruct extracellular matrix , at the same time , they also can be easier to spread to the surrounding tissue by the enhanced migration ability .

而是可以引起真皮的炎症反应，尤其是激活血管周围的巨噬细胞和肥大细胞，引起免疫细胞浸润，炎性介质以及细胞因子可导致组织溶解酶如弹性蛋白酶、胶原酶的释放，进而缓慢溶解上述基质成分。

It can cause dermal inflammation , especially activated perivascular macrophages and mast cell infiltration . Inflammatory mediators and cytokines can lead to tissue lytic enzymes releasing , such as elastase , collagenase , and slow dissolute the matrix components .

乙酸溶解-酶消化法制备医用胶原膜

Preparation of medical collagen membrane with acetic acid dissolution and enzyme digestion

本文通过对溶解和酶解两个关键工序的研究，确定了以鱼粉为原料生产鱼蛋白复合多肽的最佳工艺。

This paper studied on two key procedure of fish protein complex polypeptide ( the dissolution and enzymolysis of fish powder ), and the optimum conditions were determined .

沉淀-溶解可调节酶载体的合成及表征

Synthesis and characterization of insoluble soluble reversible carriers for immobilization of enzyme

方法：从猪肾皮质中分离刷状缘微绒毛膜，用正丁醇溶解膜结合的酶，经硫酸铵沉淀，DEAEsephacel、ConASepharose、SephadexG200及抗γ谷酰基转移酶（GGT）免疫亲和层析提纯ALP。

Methods : The ALP purification procedure included isolation microvillus of porcine kidney cortices by solubilization the membrance-binding enzymes with 1-butanol , precipitation with ammonium sulfate , chromatography on DEAE-Sephacel , ConA-Sepharose , Sephadex G-200 and a immuno-affinity column of anti-GGT antibodies .

PTE35b（+）-phyA／BL21（DE3）系统表达的蛋白约占菌体总蛋白的20%，主要以溶解形式存在，酶的活力较低。

In PTE35b ( + ) - phyA / BL2l , the recombinant protein in the form of soluble protein and reached 25 % in thallus protein , The biology activity of refolded protein was very low .