聚合酶链反应技术

用聚合酶链反应技术探测人载脂蛋白AI基因多态性

Polymorphism of apolipoprotein RI gene studied with polymerase chain reaction

同期用定量聚合酶链反应技术测定CHB患者外周血HBV载量。

The loads of HBV were measured with fluorescence quantitative polymerase chain reaction ( FQ-PCR ) .

用聚合酶链反应技术检测泌尿系疾病患者尿中疱疹病毒DNA

Detection of Herpesvirus DNA in Urine Pellet of Patients with Urinary System Disorders

目的：评估荧光定量聚合酶链反应技术检测梅毒螺旋体DNA的应用价值。

ObjectiveTo assess the application value of fluorescence quantitative polymerase chain reaction for detection of treponema pallidum DNA .

应用依赖随机化末端连接物聚合酶链反应技术研究重铬酸钾对大鼠肺p53基因DNA损伤

Applying randomized terminal linker-dependent PCR to detect the DNA damage of p53 gene in rat

用聚合酶链反应技术构建人可溶性肿瘤坏死因子受体cDNA克隆

Construction of human soluble tumor necrosis factor receptor cDNA clone by PCR

免疫组织化学-激光显微切割-聚合酶链反应技术在胃癌石蜡切片中检测p53突变的应用

Application of immunohistochemistry-laser microdissection-PCR technique in detecting p53 gene mutation in paraffin sections of gastric cancer

顺序特异性引物聚合酶链反应技术对HLA-DRDNA的分型

Typing for HLA-DR DNA by polymer - ase chain reaction with sequence-specif - ic primers

提取各类细胞总RNA，行逆转录聚合酶链反应技术（RT-PCR）鉴定肌钙蛋白-T（1Tn-T1）的RNA表达。

Total RNA was isolated and reverse transcriptase-polymerase chain reaction ( RT-PCR ) was performed to detect the expression troponin-T 1 genes .

方法应用半巢式-聚合酶链反应技术检测肺癌患者服用卡宁前后外周血bcl-2表达水平。

[ Methods ] bcl-2 gene analysis was performed in patients with lung cancer by semi-nested-polymerase chain reaction before and after treatment .

运用cDNA微阵列结合荧光半定量聚合酶链反应技术，首次大规模分析人胚发育过程中肝脏脂代谢相关基因的表达情况，比较孕早、晚期人胚肝脂代谢相关基因的表达谱。

Aim To analyze the expression profile of lipid metabolism related genes in human fetus liver by cDNA microarray and fluorescent semi quantitative PCR .

应用半定量逆转录聚合酶链反应技术，检测培养细胞中IFNγ和IL4mRNA的表达水平。

The mRNA levels for IFN γ and IL 4 in culture cells were evaluated using semi quantitative reverse transcription polymerase chain reaction .

应用任意单引物聚合酶链反应技术，从水稻WA型雄性不育系的线粒体DNA中得到一个特异的扩增片段R2－630WA。

A specific amplified mtDNA fragment R 2 630 WA was obtained from wild abortive type ( WA ) male sterile cytoplasm by AP PCR technique .

采用免疫组织化学法和逆转录聚合酶链反应技术检测卵巢组织VEGF蛋白质和mRNA的表达。

With immunohistochemistry and reverse transcription poly - merase chain reaction , expressions of VEGF proteins and their mRNA in the ovarian tissue of each group were detected .

方法：用序列特异性引物聚合酶链反应技术（PCR-SSP）检测TNF等位基因及基因型。

Method : Allele and genotype frequencies were determined by polymerase chain reaction and sequence specific primers ( PCR-SSP ) .

方法：应用虫媒病毒的通用引物逆转录-聚合酶链反应技术（RT-PCR）。

Methods : Serum samples from 168 patients with unknown fever and the samples mosquitoes were assayed by reverse transcription polymerase chain reaction ( RT-PCR ) .

顺序特异引物聚合酶链反应技术HLA－DR1，DR51组的基因快速分型

Rapid genotyping for hla - Dr 1 , Dr 51-associated group by PCR-amplification with sequence-specific primers

方法采用外周血染色体G显带、C显带技术和多重聚合酶链反应技术，对2例无精症患者进行了细胞遗传学和分子遗传学检测。

Methods Peripheral blood samples were taken from two patients with azoospermia , and then were examined by use of G banding , C banding cytogenetic analysis and multiplex polymerase chain reaction ( PCR ) microdeletion analysis .

通过逆转录聚合酶链反应技术，以GAPDH为内参照，测量SR的钙调控蛋白mRNA表达量。

The mRNA amount of calcium regulatory proteins genes was measured by reverse transcription polymerase chain reaction and normalized to the mRNA levels of GAPDH .

在相应时间点断头取脑，逆转录聚合酶链反应技术检测大鼠皮质caspase9mRNA的表达，并与对照组（C组）比较。

The rats of group B were treated with EPO after globe ischemia-reperfusion . Brain tissues were taken out after execution at different time point . The expression of caspase-9 mRNA was detected with reverse transcription PCR ( RT-PCR ) technique .

方法：使用荧光聚合酶链反应技术检测41例病儿单纯疱疹病毒DNA，巨细胞病毒DNA，柯萨奇病毒RNA，对其中为阳性的病儿进行脑电图检查，比较检查结果。

Methods : Herpes virus DNA , cytomegalo virus DNA , coxsackie virus RNA were detected by the Fluorogenic Quantitative PCR technology in 41 children with TORCH infection and EEG was done in those patients with positive results .

方法：用差异显示反转录-聚合酶链反应技术分析轻度修饰低密度脂蛋白诱导下人血管内皮细胞的基因表达差异，并用反向Northern分析证实差异显示基因片段。

METHODS : DD - reverse transcription ( RT ) - PCR technique was used to analyze genetic expression difference of human vascular endothelial cells induced with mm-LDL and reverse Northern analysis was performed to testify DD genetic fragments .

方法以41例消化系统肿瘤患者（肿瘤组）和32例非肿瘤性消化道疾病患者（对照组）为对象，应用逆转录-套式-聚合酶链反应技术检测血液标本CEAmRNA。

Methods There were 41 patients with digestive system tumor and 32 patients with non-tumor digestive system diseases in the study . CEA mRNA in blood was detected by reverse transcriptase-nested primers-polymerase chain reaction .

分别在大鼠出生后第10天和第21天，两组每窝各取1只幼鼠处死后取海马组织，采用逆转录聚合酶链反应技术测定海马前脑啡肽mRNA的表达。

Ten animals ( 1 rat from each of the 10 litters ) in each group were killed on the 10th and 21st day after birth respectively . Hippocampi were removed for determination of proenkephalin mRNA expression by RT-PCR .

方法：自行设计合成引物建立顺序特异性引物聚合酶链反应技术，并对110例肾移植供体作HLA-DR基因分型。

Methods : Design and synthesize primers to establish the polymerase chain reaction sequence-specific primer ( PCR-SSP ) method based on DNA extraction for HLA-DR locus genotyping .

方法利用CD14基因组成的特点，采用聚合酶链反应技术以人外周血单个核细胞基因组DNA为模板扩增获取人CD14基因。

Methods Based on the character of CD14 gene , the authors amplified the CD14 gene directly from genome DNA of human mononuclear cell in peripheral blood by PCR for the first time .

方法随机抽查110名无偿献血员所供血液；以聚合酶链反应技术检测血清中HPVB19DNA的表达。

Results PCR assay was applied to detect the serum HPV B 19-DNA expression in randomly selected 110 blood donors .

作为对照.其血清及周围血单核细胞（PBMCs）中正、负链HCVRNA用套式反转录&聚合酶链反应技术检测。

The plus and minus strands of HCV RNA in sera and peripheral blood mononuclear cells ( PBMCs ) of these patients were tested by means of nested reverse transcription-polymerase chain reaction ( nested RT-PCR ) .

目的应用聚合酶链反应技术（PCR）快速检测耐甲氧西林葡萄球菌（MRS）的mecA基因，与相关抗生素药敏表型试验对照分析，为临床合理使用抗生素提供实验室依据。

Objective To detect mecA gene of MRS ( methicillin resistant staphylococcus ) with PCR and to compare the results with conventional microbiologic method so as to provide the scientific basis for reasonable application of antibiotics .

方法：采用单克隆抗体一步法和顺序特异引物聚合酶链反应技术（PCR-SSP），对78例肾移植受者进行随机性HLA-A、B、DR位点配型。

Methods : HLA A , B , DR matching by one step monoclonal antibody and polymerase chain reaction with sequence specific primers ( PCR SSP ) were made for 78 randomly selected donor recipients of cadaveric renal transplantation .