ALPA

Selection and breeding of the strain for production of acid ALPA - amylase

酸性α-淀粉酶生产菌株的选育的初步研究

But the difference of ALPA activity in rhizosphere is not significant among the genotypes .

根际碱性磷酸酶在基因型间差异不显著。

Low phosphorus condition also made ALPA activity in rhizosphere rise , the contents of soluble protein and soluble P in leaf reduce .

低磷胁迫下，根际碱性磷酸酶活性升高，叶片中可溶性蛋白含量、可溶性磷含量降低。

Cloning , Expression , Purification and Identification of Conservative Region of Four Helicobacter pylori Adhesin Genes in AlpA Gene

幽门螺杆菌AlpA基因中四种黏附素基因保守区的克隆、表达、纯化及鉴定

The result of nucleotide sequence analysis showed that the sequence of alpA DNA was almost the same as that of the published in GenBank .

结果DNA序列分析表明，所克隆的alpA基因序列与GenBank公布的基本一致。

Conclusion Cloning and high-expression of adhesin AlpA and primary confirmation of its immunogenicity lay the foundation for H.pylori gene engineering vaccine preparation and adhering mechanism research .

结论AlpA克隆、高效表达及其免疫原性的初步证实，为Hp基因工程疫苗的研制和粘附机制的研究打下了基础。

The alpA DNA fragment was amplified by PCR and then inserted into the plasmid pET-22b ( + ) for sequencing .

方法利用PCR技术扩增alpA，并将其定向插入pET-22b（+）载体中通过DNA序列分析仪进行核苷酸分析。

To obtain DNA fragment of Helicobacter pylori ( Hp ) adhesin gene alpA and to insert the amplified fragment into the plasmid pET-22b ( + ) for nucleotide sequence analysis .

目的获取幽门螺杆菌黏附素基因alpA，并将它克隆到质粒pET-22b（+）中进行核苷酸序列分析。