Stigmasterol

A Study on Technology of Extracting and Purification of Stigmasterol

豆甾醇提取纯化工艺研究

HPLC Analysis of Stigmasterol and β - Sitosterol in Phytosterols

混合植物甾醇中豆甾醇和β-谷甾醇的高效液相色谱分析

Separation and Purification of Stigmasterol and β - Sitosterol from Phytosterol by the Crystallization Method

结晶法分离精制混合植物甾醇中β-谷甾醇和豆甾醇

Nine compounds structure were identified , stigmasterol ,β - sterol , azelaic acid were also obtained from this genus the first time .

确定了9种化合物的结构，其中豆甾醇、β-谷甾醇、壬二酸等为首次在本属真菌中分离得到。

The results showed that they both are containing stigmasterol ,β - sitosterol , and small quantities of campesterol .

结果表明，两种组分均主要含有豆甾醇和β-谷甾醇以及少量菜油甾醇物质。

Stigmasterol acetate , β - sitosterol acetate and mixed phytosterol acetates were analyzed by HPLC .

应用高效液相色谱对豆甾醇乙酸酯、β谷甾醇乙酸酯和混合甾醇乙酸酯进行了分析测定；

And the Compounds of stigmasterol , ethyl gallate and butanedioic acid were obtained from this plant first time among these componds .

其中豆甾醇、没食子酸乙酯、苯甲酸为首次从该植物中分离得到。

The products were identified to be brassicasterol and stigmasterol respectively based on their chromatographic and spectral properties as well as by comparison with the authentic samples .

用色谱、光谱以及与标准品对照等方法鉴定代谢产物的结构分别为菜子甾醇和豆甾醇。

Various factors affecting rebinding of cholesterol are discussed along with recognition specificity studies on its analogues of stigmasterol and ergosterol through estimation of UV-Vis and electrochemical impedance spectroscopy .

讨论了影响胆固醇结合的几个因素，并通过紫外-可见光谱和电化学阻抗谱对胆固醇及其类似物的特异性识别进行了研究。

The phytosterol content in the purified sample was 85 % , including 42.3 % Betasitosterol 、 18.1 % stigmasterol 、 24.5 % campesterol .

精制甾醇中大豆总甾醇含量为85%，其中β-谷甾醇42.3%、豆甾醇18.1%、菜籽甾醇24.5%。

Results : The contents of stigmasterol were much the same in three kinds of package , but the contents of microbe and water in polyethylene package increased more rapidly than those in vacuum package and paper-plastic package .

结果：各包装饮片的豆甾醇的含量基本无变化，聚乙烯塑袋包装的饮片微生物和水分均远高于真空复合包装和纸塑包装。

The results show that the esterification is carried out completely under the experimental condition and the relationship between the ratio of the feature peak area of IR spectra and the ratio of the contents of stigmasterol acetate and β sitosterol acetate is linear .

结果表明：在实验条件下甾醇酯化反应完全，β-谷甾醇乙酯和豆甾醇乙酯的红外特征吸收峰面积比与两者的含量比成线性关系。

The experimental results show that the content of b-sitosterol is more than 87 % after the third fractional crystallization in cyclohexanone and the content of stigmasterol is more than 92 % after the fifth crystallization in n-pentanol or cyclohexanone .

实验结果表明，以环己酮为溶剂，混合植物甾醇经过3次分级结晶后，b谷甾醇含量达到87%；以环己酮或正戊醇为溶剂，通过5次重结晶后，豆甾醇含量达到92%。

Results : From the petrol-ether fraction of the EtOH extract of Semen Celosiae , 3 compounds were isolated and identified as β - si-tosterol ( 1 ), palmitic acid ( 2 ) and stigmasterol ( 3 ) .

结果：从青葙子乙醇提取物的石油醚部位分离得到化合物1～3，分别鉴定为β-谷甾醇（β-sitosterol）、棕榈酸（Palmiticacid）、豆甾醇（stigmasterol）；

Phytosterols , including J3-sitosterol , stigmasterol , campesterol and brassicasterol mainly , were obtained commonly from by-product of refining vegetable oil . Phytosterols have been use as a novel functional food ingredient with plasma cholesterol lowering activity which works by inhibiting the absorption of cholesterol .

从油脂精炼副产物中提取的植物甾醇主要包括β-谷甾醇、豆甾醇、菜油甾醇和菜籽甾醇，能抑制人体对胆固醇的吸收，是一种降低血清胆固醇的功能活性成分。