Widal reaction

Clinical value of positive and false positive Widal reaction

肥达反应阳性和假阳性的临床意义

The collected samples were cultured for the identification of intestinal pathogens with Widal reaction done .

方法采用现场流行病学及病例对照方法进行调查，对采集的标本进行肠道致病菌培养和肥达反应检测。

Contrast observation on typhoid bacilli H antigen detection , widal reaction and bacterial culture for diagnosing typhoid

伤寒杆菌H抗原检测、肥达反应与细菌培养对伤寒诊断的对照观察

〔 Methods 〕 The pathogenic bacteria were cultured using the blood and bone marrow excrement biochemistry , serum agglutinin test , Widal reaction , ELISA and medicine sensitivity test .

〔方法〕采用血、骨髓、粪便病原菌培养方法、生化反应、血清凝集实验、肥达氏反应、抗原酶联免疫实验方法和药物敏感性试验。

METHODS Minigel column technique ( MGCT ) and Widal reaction ( WR ) were used to detect the antibody among 32 suspicious typhoid and paratyphoid patients .

方法采用微柱凝胶技术（MGCT）及肥达反应法（WR）分别检测32例疑似伤寒及副伤寒病患者。

The Schuffner test was inconclusive but the Widal reaction supported typhoid fever and he was admitted to Ruchill Hospital on13 December .

薛夫讷氏试验没有确定结果，而肥达氏反应支持为伤寒。患者于12月13日住进鲁奇尔医院。

Methods : We examined and observed 52 patients with typhoid and 20 normal controls by bacterial culture , Widal reaction , and enzyme-linked immunoassay for detection of typhoid bacilli H antigen .

方法该文采用细菌培养、肥达反应、伤寒杆菌H抗原酶联免疫检测检查52例伤寒患者，20例正常人，进行对照观察。

Results : The positive coincident rate of bacterial culture , Widal reaction , and enzyme-lin ked immunoassay for detection of typhoid bacilli H antigen for diagnosing typhoid were 34.6 % , 59.6 % , 86 % , respectively .

结果细菌培养、肥达反应、伤寒杆菌H抗原的酶免疫检测对伤寒患者的诊断的阳性符合率分别为34.6%，59.6%和86%。

Dot-ELISA was ten times more sensitive than Widal 's reaction , moreover , it had no cross-reaction .

Dot-ELISA法比肥达氏反应敏感10倍以上，且无交叉反应。

Result The sera from 21 patients with typhoid fever were detected , 8 samples showed posi-tive by Widal 's reaction , but 21 were positive by immunoblot assay .

结果：共检测确诊早期伤寒病人血清21例，肥达氏反应Hd阳性8例，免疫印迹酶联试验Hd阳性21例。

Methods A Dot-ELISA method was used to determine the specific antibodies of envelope antigen , O antigen and H d antigen in Salmonella . Then the results were compared with the results of Widal 's reaction .

方法采用Dot-ELISA测定伤寒沙门菌包膜抗原V、菌体抗原O和鞭毛抗原Hd的特异性抗体，并与肥达氏反应相比较。

Objective To explore the clinical value of anti serum typhoid antibody ( St Ab ), Widal ′ s reaction ( WR ) and bacteriological culture in the diagnosis of typhoid .

目的：探讨抗伤寒抗体（St-Ab）、肥达反应和细菌培养诊断伤寒的临床价值。