In error prone PCR , higher concentration of Mg 2 + increased the mutation rate . Increased content of dTTP and dCTP had better effect than that of dATP and dGTP .
在错配PCR中,增加Mg2+浓度可提高突变率,增加dTTP和dCTP浓度的致突变效果优于增加dATP和dGTP的效果。