gapdh

The effect of targeting inhibition of GAPDH expression by RNA interference on rotenone induced cell death

RNA干扰靶向抑制三磷酸甘油醛脱氢酶表达对鱼藤酮所致神经毒性的影响

The functional diversity of GAPDH is discussed in this review .

本文主要讨论了GAPDH的功能多样性。

Advances in study on functional diversity of GAPDH

GAPDH功能多样性研究进展

The significant decrease of Gapdh after SSH suggested high subtractive efficiency in our experiment .

结果，Gapdh的表达丰度在消减杂交结束后明显降低，表明我们的消减效率很高。

Relationship Between the Expression of GAPDH Gene and Anther Abortion of Physiological Male Sterile of Wheat

GAPDH基因表达与小麦生理型雄性不育花药败育的关系

The N - terminal amino acid of snake muscle GAPDH is valine .

蛇肌GAPDH的N-末端氨基酸为缬氨酸。

Then use Real-time PCR to detect VEGF expression , taking GAPDH as internal reference , make analysis of relative expression quantity .

然后用Real-timePcR检测VEGF基因表达，进行相对表达量分析。

Half life determination of bcl-2 and GAPDH mRNA in sinusoidal endothelial cells and Kupffer cells of rat liver

肝窦内皮细胞和枯否细胞中bcl-2和GAPDHmRNA半衰期的测定

Phylogenetic analysis shows that GAPDH is a classical nuclear gene , a house-keeping gene and a function gene .

结果表明，GAPDH是一个古老的核基因，管家基因，又是一个功能基因，是十分保守的基因。

Using GAPDH as internal control , PCR amplification was carried out and the product was analyzed by agarose gel electrophoresis .

以GAPDH基因作为内对照，进行PCR扩增，扩增产物采用琼脂糖凝胶电泳检测分析。

In the 12 weeks after transplantation group , dystrophin / GAPDH was about 0.218 ± 0.338 ;

骨髓移植12周组约为0.218±0.338；

Objective To investigate the expression patterns of Glyceraldehyde-3-phosphate dehydrogenase ( GAPDH ) in glioma and its clinical significances .

目的探讨甘油醛-3-磷酸脱氢酶在神经胶质瘤中的表达及其临床意义。

Blocking with chemically synthesized siRNA of the gene expression of GAPDH in bone marrow-derived immature murine dendritic cells

化学合成siRNA阻断小鼠骨髓源性不成熟树突状细胞GAPDH基因的表达

The fragments of GAPDH gene and the potassium channel gene were amplified using the genomic DNA of the four animals as a template .

以基因组DNA为模板，进行PCR扩增。

We designed and synthesized the primers of the human GAPDH gene and the Elaphodus cephalophus potassium channel gene .

根据人的GAPDH基因和毛冠鹿的钾通道基因设计引物。

Conclusion Time-dependent degradation of GAPDH mRNA in kidney as detected by fluorescence semi-quantitative RT-PCR technique has some value for estimation of PMI .

结论荧光半定量RT-PCR技术检测大鼠死后肾GAPDHmRNA含量变化对PMI推断具有一定的应用价值。

Conclusion : Time-dependent degradation of GAPDH mRNA in liver as detected by fluorimetric RT-PCR technique may provide a new indicator for estimation of PMI .

结论荧光RT-PCR技术检测大鼠肝组织GAPDHmRNA，在死后的降解呈现出一定的规律，可为死亡时间（PMI）推断提供新的指标。

The mRNA amount of calcium regulatory proteins genes was measured by reverse transcription polymerase chain reaction and normalized to the mRNA levels of GAPDH .

通过逆转录聚合酶链反应技术，以GAPDH为内参照，测量SR的钙调控蛋白mRNA表达量。

NOS expression was detected by using RT PCR and RNAase protection assay ( RPA ), and GAPDH was used as an internal standard .

应用RTPCR法和RNA酶保护试验（RPA）法测定2种NOS的表达，以2,3二羟基丙醛3磷酸脱氢酶（GAPDH）为内参。

The amplification efficiencies were identical for both the target gene ( PAX5 and CD19 ) and house keeping gene ( GAPDH ) .

实验证实靶基因（PAX5和CD19）与管家基因（GAPDH）的扩增效率一致，因此可以用比较循环数（Ct）法对PAX5和CD19的表达进行相对定量。

RESULTS : The specificity of products was proved to be COX-2 and GAPDH by the analysis of the melting curve of the amplified products and agarose gel electrophoresis .

结果：30份标本的扩增产物经熔解曲线分析及DNA琼脂糖凝胶电泳，证实均为COX-2和GAPDH。

Objective : To sequence the gene of glyceraldehyde 3 phosphate dehydrogenase ( GAPDH ) from Plasmodium falciparum strain FCC-1 / HN .

目的：对恶性疟原虫FCC-1HN株的3-磷酸甘油醛脱氢酶（GAPDH）基因进行序列测定。

The ratio of GAPDH ∶ HOAD suggests that not only lipid , but also carbohydrate could be utilized as energy substrates during sustained flight of the moth .

对成虫GAPDH∶HOAD活性进行分析比较的结果还显示，粘虫蛾持续飞行的能源物质既有脂类也有糖类，而不仅仅只限于脂类。

In the 4 weeks after transplantation group , only few dystrophin expressions were detected , and the dystrophin / GAPDH was about 0.095 ± 0.267 ;

骨髓移植4周组仅可见抗肌萎缩蛋白的微弱表达，dystrophin/GAPDH灰度比值约为0.095±0.267；

Objective To study the immunological characteristics induced in C57BL6 mice by nucleic acid vaccine harboring the gene encoding glyceraldehyde-3-phosphate dehydrogenase ( GAPDH ) of Schistosoma japonicum .

目的研究日本血吸虫大陆株三磷酸甘油醛脱氢酶（GAPDH）基因核酸疫苗免疫C57BL/6小鼠诱生的免疫应答特征和保护性。

The full - length cDNA of SS gene and the partial cDNA of GAPDH gene were obtained by RT-PCR , then cloned into pMD-18T Vector and sequenced .

运用RT-PCR方法扩增三七SS基因的全长cDNA序列及GAPDH基因的部分cDNA序列，分别克隆至pMD-18TVector，进行测序及序列分析。

The subtractive efficiency of 2 ~ 5 folds was obtained in the two libraries by using the housekeeping gene encoding glyceraldehyde-3-phosphate dehydrogenase ( GAPDH ) as the reference .

以持家基因GAPDH为参照指标检测消减文库的消减效率，结果发现两个文库的消减效率均高达25倍。

The preliminary crystallographic study shows that GAPDH crystal belongs to C2 space group , there is only half molecule per asymmetric unit and the molecule located at the 2-fold axis .

初步Χ射线晶体学研究确定：此酶晶体属於C2空间群，不对称单位内含有半个分子，分子坐落在二重轴上。

Conclusion Highly pure atrial cells could be obtained by LCM . Tiny amount RNA of captured atrial cells could be extracted and amplified with genes of β - actin and GAPDH .

结论LCM可以分离出纯度较高的心房细胞，提取的微量RNA可以扩增出管家基因β-actin和GAPDH。

Comparison between ribosomal 18S rRNA , GAPDH and β - actin genes as internal standard for quantitative comparison of mRNA levels in development of flounder , Paralichthys olivaceus

3-磷酸甘油醛脱氢酶、β-肌动蛋白和18SRRNA作为相对定量的内标在牙鲆发育阶段的稳定性比较