single spore isolation

The mycelia of Tremella fuciformis can be obtained by single spore isolation , mycelia isolation in medium or tissue isolation . These pure cultures can be cultured with associated fungi and thus become the master cultures of T.

用单孢分离、基本分离和组织分离获得银耳菌，银耳菌的纯培养与伴生菌长在一起成为银耳母种。

Single spore isolation analysis indicated that 35 % genotypic segregation was obtained from heterokaryotic strain .

单孢分离分析表明，异核体的分离频率约为35%。

Tissue and single spore isolation were conducted to isolate the pathogen , In natural condition , it was easier for pathogen isolation with the conidia than diseased leaf tissue .

采用组织分离和单孢分离法分离病原菌，自然条件下，用杨树角斑病菌的分生孢子分离较病组织分离易成功。

With the conservative regions and variable regions in rDNA gene sequences , the analysis of rDNA sequences is very efficient in research of fungi systemic development , classification identification and molecular detection . The method of single spore isolation is occupied in this experiments .

由于rDNA序列兼具保守区域和进化水平不同所致可变区，因此，rDNA序列分析是研究真菌系统发育、分类鉴定和分子检测非常有效的手段。

These major results are as follows : 1 . Fusarium strains collected from banana wilt disease in Zhangzhou , Fujian Province , by single spore isolation , molecular characteristics and pathogenicity tests , were 21 Foc Race 4 strains . 2 .

主要研究结果如下：1.香蕉枯萎病株采自福建省漳州，经单孢分离、致病性测定与分子鉴定，获得21个尖孢镰刀菌古巴专化型4号生理小种。