二维凝胶电泳

基于二维凝胶电泳的蛋白质定量分析技术

Quantitative Analytical Techniques of Proteins Based on Two-Dimensional Gel Electrophoresis

基于聚类统计分析的二维凝胶电泳连配方法的研究

Gel-to-Gel Matching of Two-dimensional Gel Electrophoresis Images Based on Clustering and Statistical Analysis

用十二烷基硫酸钠-凝胶电泳（SDS-PAGE）和二维凝胶电泳鉴定蛋白质纯度。

The obtained proteins were checked for the purity through SDS-polyacrylamide gel electrophoresis ( SDS-PAGE ) .

LuZH等利用二维凝胶电泳（2-DE）的方法对胰腺癌组织和癌旁正常胰腺组织进行比较蛋白质组学研究，共发现和鉴定了110种在癌组织中差异表达的蛋白。

With regard to pancreatic cancer , Lu ZH et al identified 110 differently expressed proteins in cancerous pancreatic tissue using comparative proteomic analysis .

方法：以束缚制动法制备肝郁证大鼠模型，二维凝胶电泳（2-DE）分离大鼠血清蛋白质，获得差异表达蛋白质；

Methods : Liver-QI syndrome model rats were prepared by restraints . Serum protein was separated by 2D gel electrophoresis ( 2-DE ), so as to gain differential expression protein .

目的通过聚丙烯酰胺二维凝胶电泳技术，全面展示小鼠成熟卵丘卵母细胞复合体（COC）的蛋白质表达谱。

Objective : To establish two-dimensional polyacrylamide gel electrophoresis for displaying the full-scale protein expression spectrum of mouse mature oocyte-cumulus complex and evaluate the application of this map in the study of ovarian follicle development .

目的：建立二维凝胶电泳分离结核分枝杆菌（M.TB）胞外蛋白和膜蛋白的方法。

Objectives : To establish a method for separating extracellular proteins and membrane proteins in mycobacterium tuberculosis using two dimensional gel electrophoresis .

二维凝胶电泳分离结核分枝杆菌胞外蛋白和膜蛋白

Separation of extracellular and membrane proteins in mycobacterium tuberculosis using two-dimensional gels electrophoresis

兔眼组织的二维凝胶电泳分析

Analysis of rabbit eye tissues using two-dimensional gel electrophoresis

水杨酸作用下东北红豆杉细胞的二维凝胶电泳分析

2-Dimensional Gel Electrophoresis on Protein Patterns from Suspension Cultures of Taxus cuspidate Induced by SA

二维凝胶电泳是血清蛋白质组学中的重要技术，它的分离能力强。

Two-dimensional electrophoresis that has stronger separation ability is the important technique of serum proteomics .

该技术的开发大大提高了转移效率，为二维凝胶电泳和质谱提供了一个有效的连接手段。

This technique significantly improved transference efficiency , and provided an efficient way for the connection of two-dimensional gel electrophoresis and mass spectrometry .

二维凝胶电泳、谱和生物信息学是蛋白质组学研究的三大关键技术。

The two-dimensional gel electophoresis ( 2 - DE ), mass spectrometry ( MS ) and bioinformatics are three key technologies for proteomics study .

我们对这四组研究对象进行了同位素标记的相对和绝对定量的蛋白组学研究，接着利用二维凝胶电泳及质谱分析确定我们所需的差异蛋白。

Isobaric tags for relative and absolute quantitation followed by two-dimensional gel electrophoresis and mass spectrometry were used to identify proteins that were differentially expressed among the four groups .

经二维凝胶电泳分离的蛋白质，在进入质谱之前需要长时间、繁琐的样品处理过程，难以满足自动化的需要。

Prior to mass spectrometry analysis , proteins separated by two-dimensional gel electrophoresis need a long and complex process , which makes it difficult to meet the requirements of automation .

介绍了新兴的蛋白质组学领域中较为常用的分离方法二维凝胶电泳、高效液相色谱和毛细管电泳及电色谱的基本原理。

Separation methods , such as two-dimensional gel electrophoresis ( 2DE ), High-Performance Liquid Chromatography ( HPLC ) and Capillary Electrophoresis / Electrochromatography ( CE / CEC ), are introduced in this paper , which are being used for proteomics .

2DE（二维聚丙烯酰胺凝胶电泳）技术是蛋白质组学研究中的主要分离技术，其分别以蛋白质的等电点和分子质量这两个独立的参数为基础，实现蛋白质混合物的分离。

Two-dimensional polyacrylamide gel electrophoresis is the key separation technique in proteomics research , which is designed by protein character : molecular weight and PI .

定量后，分别进行一维等电聚焦分离和二维聚丙烯酰胺凝胶电泳分离。

After quantification , the protein samples were separated by isoelectric focusing electrophoresis and then by SDS-PAGE .

蛋白质组学分析是由二维蛋白凝胶电泳和随后的计算机辅助凝胶分析并发现差异表达的蛋白。

Proteomic analysis was performed by two-dimensional protein gel electrophoresis and subsequent computerized gel analysis for detection of distinguishing patterns of protein expression .

对目前生命科学研究中的热点领域蛋白质组研究中应用的主要分离技术二维聚丙烯酰胺凝胶电泳的基本原理、应用和最新进展进行了综述。

As the major separation method in the technique platform of proteome , twodimensional polyacrylamide gel electrophoresis ( 2DE ) was optimized and developed in many ways in recent years .

结直肠癌组织的二维荧光差异凝胶电泳分析

Analysis of Colorectal Cancer by Two-dimensional Differential Gel Electrophoresis