体细胞重组
tǐ xì bāo chónɡ zǔ
- 名somatic recombination
体细胞重组-
目的建立一种快速、简便、有效且损伤小的核移植方法，研究来源于小鼠体细胞重组胚的早期发育。
Objective To establish a fast , simple , efficient and minimally invasive method for nuclear transfer ( NT ) to study the early development of mouse embryos reconstructed with cumulus cell nuclei in vitro .
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体外分离骨髓前体细胞，在重组小鼠粒细胞-巨噬细胞集落刺激因子（rmGM-CSF）刺激下，手法筛选，培养扩增小鼠骨髓来源DC（BM-DC）。
Methods : 1 . Dendritic cells were generated by culturing bone marrow precursors with recombinant mouse granulocyte-macrophage colony-stimulating factor ( rmGM-CSF ) .
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方法：分离小鼠骨髓中DC前体细胞，用5ng/ml重组小鼠粒细胞-巨噬细胞集落刺激因子（rmGM-CSF）来扩增、培养小鼠BM-DC。
Methods : DC progenitors were isolated from bone marrow of mice , and propagated in culture medium with recombinant mouse granulocyte-macrophage colony-stimulating factor ( rmGM-CSF ) .