端粒克隆、着丝粒克隆以及重叠群之间空缺的大小都经过粗线期染色体荧光原位杂交鉴定。
BAC clones corresponding to telomeres , as well as to the centromere position and the gap sizes between contigs , were determined by BAC-pachytene chromosome fluorescence in situ hybridization ( FISH ) .
克隆重叠群