免疫荧光试验

经间接ELISA和间接免疫荧光试验筛选，共获得了15株NDV杂交瘤细胞。

After screening by indirect ELISA and IFA , 15 MAbs were obtained .

间接免疫荧光试验结果显示，5株单抗和感染了CDV的Vero细胞均能发生反应。

The IFA test showed that all the 5 mABs can react with Vero cells infected by CDV .

微量免疫荧光试验和PCR在诊断肺炎衣原体急性感染中的应用

Application of microimmunofluorescence test and PCR in the diagnosis of acute Chlamydia pneumoniae infections

间接免疫荧光试验检测登革IgG的应用

Application of Indirect Immunofluorescent Assay for Detecting IgG of Dengue Virus

间接免疫荧光试验在人群埃可30型肠道病毒IgG抗体检测中的应用

Preliminary application of indirect immunofluorescence assay for detecting IgG antibodies to Echovirus 30

间接免疫荧光试验显示，HA蛋白表达于被感染细胞的胞浆中。

Indirect immunofluorescence assay ( IFA ) demonstrated that HAwas expressed by rPRV-HA in cytoplasm .

RT-PCR和间接免疫荧光试验结果显示体外培养的人巩膜成纤维细胞表达M1受体。

Results The cultured fibroblasts demonstrated mRNA expression of M1 receptor in RT-PCR .

1316只蚊分为116组，经C6／36白纹伊蚊细胞培养和单克隆抗体间接免疫荧光试验检测，全部未分离出DF病毒。

1316 mosquitoes divided into 116 groups were tested and no dengue virus was isolated .

间接免疫荧光试验证明：该组单抗测得不同人群外周血淋巴细胞阳性数与用抗B细胞单抗和直接免疫荧光法测得的结果接近。

An approximate positive rate of peripheral blood lymphocyte ( PBL ) was achieved when these McAbs and the McAb against B lymphocytes were tested by indirect immunofluorescent assay ( IFA ) .

用FITC标记的羊抗兔IgG进行间接免疫荧光试验，可检测到特异性黄绿色荧光。

Specific yellow-green fluorescence was visual in indirect immunofluorescence assay with FITC-labeled goat anti-rabbit IgG .

用逆转录聚含酶链反应（ReversetranscriptasePOLYMERASECHAINREACTION，RTPCR）和免疫荧光试验检测HBV基因在早期胚胎的表达。

Reverse transcriptase polymerase chain reaction ( RT-PCR ) and immunofluorescence assay were performed to observe the expression of the HBV gene in two-cell stage .

细胞免疫荧光试验的结果表明，ATM转染的CHO细胞内荧光强度明显低于ATN转染的CHO细胞。

Cell immunofluorescence assay results indicated that the intensity of fluorescence was significantly reduced in ATM cell compared with ATN cell .

目的：评价间接免疫荧光试验（IFA）在生殖器疱疹（GH）诊断中的应用价值。

AIM : To evaluate the application of indirect immunofluorescence assay ( IFA ) in the diagnosis of genital herpes .

上述杂交瘤细胞涂片后与日本血吸虫感染兔血清进行间接免疫荧光试验（IFT），以探讨凝集机制。

Immunofluorescent test was carried out with the hybridoma cells and schistosome-infected sera .

间接免疫荧光试验（IFA）显示F12D可识别培养的FCC1/HN。

Moreover , the antibody also recognized the cultured parasites of P.falciparum by indirect immunofluorescent antibody test ( IFA ) .

间接免疫荧光试验结果表明重组NP多克隆抗体能够识别牛副流感病毒3型在感染细胞中表达的NP蛋白，而且在稀释度为1：2560时仍具有识别能力。

Indirect immunofluorescence test showed that the antibody could identify the NP protein expressed by bovine parainfluenza virus type 3 in infected cells , even in the dilution of 1:2560 .

免疫荧光试验表明，抗AFPiRNA能诱导小鼠脾细胞表达膜AFP抗体或受体。

In addition , the immunofluorescence revealed that AFP-specific iRNA is capable of inducing the expression of membranous anti-AFP antibodies or AFP receptors on mouse spleen cells .

经BUdR加压筛选3次后，重组病毒分别用PCR、间接免疫荧光试验和westernblot进行鉴定，并进行小鼠免疫研究。

The recombinant virus was identified by PCR , indirect immunofluorescent assay ( IFA ) and Western blot after three times of pressure selection with BUdR , and the mice immunization experiment was performed .

结果免疫荧光试验显示A12表达产物与感染CVS的鼠脑细胞有强的荧光反应。

It was found that the strong reactivity of ScFV-A12 to the mouse brain cells infected with rabies virus CVS could be demonstrated by IFA .

方法采用微量免疫荧光试验，对149例急性呼吸道感染患儿及111例对照组儿童（55例同期住院的非呼吸道疾病患儿，56例健康儿童）进行血清肺炎衣原体特异性抗体IgG，IgM检测。

Methods The specific serum IgG , IgM antibodies against CP were detected in 149 children with acute respiratory disease and in 111 control cases ( 55 with non-respiratory disorders and 56 healthy children ) .

自身抗体检测采用间接免疫荧光试验（IIFT）。

Auto-antibodies were detected by indirect immunofluorescence test ( IIFT ) .

目的：建立检测血清中人疱疹病毒6型（HHV6）IgG的间接免疫荧光试验（IFA）。

AIM : To establish an indirect immunofluorescence assay ( IFA ) for detection of serum IgG antibody against human herpesvirus 6 ( HHV 6 ) .

目的：探讨肺炎衣原体（Cpn）急性感染在呼吸道疾病中的重要性。方法：用微量免疫荧光试验检测血清中Cpn抗体IgG。

Objective To evaluate the role of acute infection of Chlamydia pneumonia ( Cpn ) in respiratory diseases Methods Microimmunofluorescence test for detection of IgG antibodies for Cpn in serum was used .

中；将重组质粒转染到宿主细胞COS7细胞中，通过间接免疫荧光试验（IFA），证明该基因在宿主细胞中得到表达。

Then the recombinants were transfected into COS-7 , it was proved that the env gene were expressed in the host by IFA .

本文用乳胶凝集试验（PA）、免疫荧光试验（IFA）和蛋白印迹试验（WB）对103份食蟹猴血清作了STLV－1抗体检查。

103 Cynomolgus monkeys were tested for the serum antibody of STLV-1 by Gelatin particle agglutination ( PA ), Immunofluorescence assay ( IFA ) and Western blot ( WB ) .

采用微量免疫荧光试验检测血清CP抗体IgG、IgA、IgM，采用流式细胞仪测定两组的外周血CD3+、CD4+、CD8+T淋巴细胞。

Chlamydia pneumoniae specific serum antibodies IgG , IgA and IgM were measured by microimmunofluorescence ( MIF ) test ; flow cytometry was used to analyze the frequencies of CD3 + , CD4 + and CD8 + lymphocytes in patients with COPD and controls .

此外，采用微量免疫荧光试验（MIF）对96例呼吸道感染者与48例健康献血员的血清学分析表明：83.4%的患者肺炎衣原体IgG抗体阳性，与对照组比较，统计学上有非常显著差异。

In addition , sera from 96 patients and 48 healthy blood donors have been assayed with MIF test . The results showed that C. pneumonia IgG antibody of 83.4 % patients was positive , demonstrating very significant statistical differences if compared with the control and suggesting C.

质粒提纯后，在脂质体作用下转染BHK-21细胞，通过直接免疫荧光试验和间接免疫荧光试验分别检测P32和CD58在BHK-21中的表达。

After plasmid purification , the acquired recombinant plasmids were transfected into BHK-21 cell with liposome mediated method . the expression of P32 and CD58 were tested by direct immunofluorescence assay ( IFA ) or indirect immunofluorescence assay .

结果：毕赤酵母GS115培养上清液中有目的蛋白的表达，分子量约为44KD，间接免疫荧光试验显示在大鼠肋间肌细胞间有荧光出现。

Results : The target protein was secreted in the supernatant of expression culture medium . Its molecular weight was about 44 KD . There was fluorescence between the mouse intercostals muscle cells .

与免疫荧光试验（IFT）的平行对照表明，无论用何种底物，IGSS的敏感性均显著高于IFT，而特异性与IFT相同。

This method was compared with the current ones in respect to the method for staining and the selection of dsDNA antigen substrate . The results showed that compared with immunofluorescence test ( IFT ) , IGSS has significantly high sensitivity ( p0.05 ) and similar specificity ( p0.05 ) .