农杆菌转化法

在过去的几十年间，研究人员用农杆菌转化法获得了多种转基因植物，该转化法依赖于农杆菌中存在的Ti质粒。

Scientists have used Agrobacterium-mediated genetic transformation to generate transgenic plants , and this method depends on the Ti plasmid .

利用基因枪和农杆菌转化法可将大豆铁结合蛋白基因导入旱稻基因组中，并使转基因稻米铁含量增高。

The soybean ferritin gene can be transferred into upland rice using the method of Biolistic and Agrobacterium-mediated transformation , which increased iron concentration in the transgenic plant seeds .

转化三种植物表达载体的番茄再生植株的获得：通过农杆菌转化法对三个番茄栽培品种进行转化，各个载体和品种都获得了大量的转基因植株。

Attainment of the regenerated plants transformed by three plant expression vectors : three vectors were transformed into three tomato cultivars by Agrobacterium mediated-transformation method . A lot of putative transgenic plants were obtained from the regenerated plants .

通过农杆菌介导转化法侵染270块的愈伤组织，获得6株再生植株，转化率2.2%。

Only 6 plantlets were regenerated form 270 calli by Agrobacterium-mediated transformation method , the transformation frequency was 2.2 % .

通过根癌农杆菌和叶盘转化法将curcin基因导入烟草。

At last , we lead gene Curcin in tobacco by the conversion method of Agrobacterium and Leaf discs of tobacco .

将这两个载体转化农杆菌，再利用农杆菌介导转化法将启动子片段和报告基因GUS的融合基因导入烟草，组织培养获得转基因植株。

Two vectors were transformed into agrobacterium , and the transformed agrobacterium was used to bring the fusion gene of promoter fragment / reporter gene GUS into tobacco . Transgenic plants were gained by tissue culture .