包含体

以包含体形式由大肠杆菌表达的基因重组人白细胞介素2（rhIL－2），经纯化后其纯度已达95%以上〔1〕。

Human recombinant interleukin-2 ( rhIL-2 ) in the form of inclusion bodies derived from E. coli was purified with the homogeneity of more than 95 % .

从大肠杆菌包含体中提取有活性的人溶菌酶的研究

The Studies on Recovery of Biologically Active Recombinant Human Lysozyme from Inclusion Bodies in Escherichia coli

透析法和稀释法复性重组人Cu，Zn-SOD包含体

Renaturation of Recombinant Human Cu , Zn-Superoxide Dismutase by Dilution and Dialysis

特发性帕金森氏病（PD）是一种缓慢进展的神经系统变性疾病，病理改变见于中脑黑质纹状体，神经细胞内存在嗜酸性包含体，又称Lewy氏小体。

Idiopathic Parkinson 's disease ( PD ) is a slowly progressive disorder of degeneration of nerve system .

面模型在线模型的基础上虽增加了面的信息，但还不包含体的信息，因此发展了实体模型并逐步在CAD中得到了广泛的应用。

Developed from wireframe model , the surface model lacks information about solids , although it adds information of surfaces . So a solid model has been developed and widely used in CAD systems .

进而将该方法应用到重组增强型绿色荧光蛋白（EGFP）的复性中，发现EGFP包含体的复性收率提高近一倍，并且在辅助复性的同时还具有纯化的效果。

The method was further applied to the renaturation of enhanced green fluorescent protein ( EGFP ) inclusion bodies . Like-charged exchanger increased the refolding yield by almost 100 % .

ColiDH5α（pIB-hIL-6），经过发酵培养、表达和包含体的分离纯化等步骤，获得了N端缺失5个氨基酸的rhIL-6纯品。

Coli DH 5 α( pIB-hIL-6 ) and purification of the inclusion body , we obtained the pure protein of recombinant human Interleukin-6 ( rhIL-6 ), characterized by the first 5 amino acids at the N-terminal end .

关于昆虫包含体病毒染色方法的探讨

Discussion on staining methods in inclusion body of insect virus

包含体&流行性出血热病毒形态学的重要特征

Inclusion body - an important morphological marker of EHF Virus

外源蛋白高效表达易形成包含体，通过对包含体的溶解、复性和纯化过程的研究，最终获得有活性的β-内酰胺酶并达到90%以上的纯度。

Finally β - lactamase was purified up to 90 % homogeneity .

表达后的融合蛋白以包含体的形式存在，其含量达到了菌体总蛋白的20%左右。

The fusion protein was existed as an inclusion body .

包含体蛋白质的复性研究进展

Advance in vitro folding of inclusion body proteins

在病叶的超薄切片中见到了大量玩具风车状及束状包含体，显示HMMV是马铃薯Y病毒组成员。

It was shown that HMMA is a member of potato Y virus group .

目的：减少大肠杆菌发酵过程中包含体的形成。

OBJECTIVE : To reduce the formation of inclusion body in the course of fermentation of E.

腺病毒肺炎肺内包含体与淋巴结反应性变化的关系

Relationship between intrapulmonary inclusion bodies in adenoviral pneumonia and changes in the reactivity of lymph nodes

包含体表达基因工程牛生长激素提取、变复性及纯化的研究

Study on the extraction , denaturation , renaturation and purification of inclusion body-expressed bovine growth hormone

描述和讨论了包含体与细胞及病毒发育成熟的关系。

The possible relationship among the inclusion bodies and their bearing to virus morphogenesis are discussed .

将两个目的蛋白的包含体进行酵母凝集实验，发现酵母凝集能力更强。

We found the ability of Saccharomyces cerevisiae agglutination of two purposes protein inclusion body is stronger .

构建的蛋白质分泌性表达的载体-宿主系统及合适的培养条件为易形成包含体的蛋白质的高效表达提供了一条新的途径。

This system provides a new approach for the expression of the proteins easy to form inclusion bodies .

重组蛋白分泌到周质空间或胞外培养基中较之在胞内以包含体形式表达有许多优势。

The secretion of recombinant protein to periplasm or extracellular medium has more advantages than the expression intracellular as inclusion bodies .

12%SDS-PAGE表明，表达产物大小为48.5kDa，主要以包含体形式存在。

12 % SDS-PAGE showed that the expression product with molecular weight 48.5 kDa was mainly in the form of inclusion body .

病叶超薄切片中有风车状包含体，属马铃薯Y病毒组的成员。

The ultra-thin sections of infected leaves showed pinwheel inclusion bodies , indicating that the virus is a member of the potato Y group .

柱层析法可以获得纯度95%以上的融合蛋白，包含体复性率80%以上。

The fusion protein with purity above 95 % could be obtained by column chromatography , the refolding rate of inclusion body above 80 % .

昆虫包含体病毒特异性的鉴别染色方法为我们准确地在光学显微镜下检查包含体病毒提供了有益的方法。

The special differential staining methods for inclusion body of insect virus affords us a useful methods , which check up on inclusion body of insect virus under the light-microscope .

结论：在痘病毒感染胞核内观察到四种核内包含体和核小体，这些特殊结构的形成与胞核功能活跃有关。

Conclusion : There are four kinds of intranuclear inclusion and nuclear bodies in nucleus of cells infected by poxvirus ; their formation may be related to active condition of nuclear functions .

在线面连接问题中，电流展开函数包含体展开函数、线展开函数和连接点展开函数三类。

When analyzing a system of conducting bodies interconnected by wires , the current expansion functions consist of three classes , body expansion functions , wire expansion functions and junction expansion functions .

本文利用双盲法分别观察了腺病毒肺炎的肺组织及淋巴结组织切片，并按一定的方法判定包含体的数量及淋巴结功能。

Both the lung and lymph node tissues of patients with adenoviral pneumonia were observed , using a double-blinded meth , to determine the amount of inclusion bodies and function of the lymph nodes .

离心收集重组体菌体，超声波破碎离心，发现沉淀中含有目的蛋白，说明目的蛋白以包含体的形式表达，没有活性，需要复性才能分离纯化。

Collecting recombinant bacterias and ultrasonic crushing centrifugal , we found that the purpose protein in precipitation . It illustrated that he purpose protein was expressed in form of IB with no activity , needing renaturation to separation and purification .

在接种病毒后8～15天出现了特异性包含体，主要分布在细支气管和呼吸性支气管上皮细胞，也可见于大支气管上皮细胞及腺细胞。

Specific inclusion bodies appeared 5 - 8 days after inoculation with 3 - AdV and were mainly distributed in the bronchioles and respiratory bronchiolar epithelial cells . They were also seen in the epithelial cells of the mucosa and glands of bronchi ;

应用CAD软件在计算机上建立包含修复体上部结构和种植体的下颌后牙区骨块。

A posterior mandible segment with an implant and a superstructure were modeled on a personal computer with CAD software .