双单倍体

马铃薯双单倍体诱导研究

A Study on Dihaploid Induction of Potato

分子标记与QTL间连锁的检测与估计Ⅱ.利用双单倍体（DH）群体

Methods for detection and estimation of linkage between a marker locus and quantitative trait loci ⅱ . using double haploid ( DH ) population

利用SSR标记检测来源于玉米孤雌生殖的双单倍体

Identification of Doubled Haploid Plants from Maize Gynogenesis by SSR Molecular Marker

02428/HX-3双单倍体（DH）群体对水稻白叶枯病的抗性鉴定

Identification of Resistance of 02428 / HX-3 Doubled Haploid Population to Bacterial Leaf Blight

利用RFLP标记分析一对水稻籼粳交双单倍体的基因型

Genotypic mapping using RFLP markers on doubled haploids derived from a cross between Indica and Japonica Rice

通过未授粉子房离体培养途径和辐射花粉授粉诱导雌核发育途径诱导甜瓜单倍体植株，再经染色体加倍迅速获得纯合的双单倍体植株（DoubleHaploid）。

Unpollinated ovary in vitro culture ways and pollen irradiation induced gynogenesis pathway can induce melon haploid plants . Homozygous double haploids will be obtained via chromosome doubling rapidly .

从255个双单倍体株系抗旱鉴定结果看，该群体表型呈单峰连续分布，符合正态分布，表明叶片卷曲度在DH群体中呈现数量遗传的性状。

The result indicates that the leaf rolling presents quantative genetic character in the DH population because the phenotype distribution accords with normal school .

若象大白菜、甘兰型油菜那样通过游离小孢子培养的方法获得双单倍体（DoubleHaploid；DH）植株，可大大缩短纯化菜心品种所需的时间并减少工作量。

With the method of anther and pollen culture , it can be got double haploid plant . Therefore , it may greatly shorten the time of purification in the breeding .

用大量资料论述了马铃薯双单倍体、四倍体再合成、2n配子在育种中应用研究进展概况.并论述了近10年2n配子育种成就。

By using large amount of information , the recomposition of potato dihaploids and tetraploids , and the progress of the application of 2n-garnetes in breeding are discussed in detail .

同时概述了集群分离分析法在F2代、回交、双单倍体、重组自交系等不同分离群体中的应用情况，并对不同分离群体的优缺点进行了比较。

At the same time the applications of the method in the different segregating generations such as the F2 , backcross , RIL , DH and so on were introduced and compared .

利用青花菜（BrassicaoleraceaVar.italica）与芥蓝（BrassicaoleraceaVar.alboglabra）杂交后代双单倍体（DH）群体为材料，通过AFLP技术构建了一个甘蓝类作物较高密度的遗传连锁图谱。

A high-density genetic linkage map of Brassica oleracea was constructed using AFLP markers based on a Brassica oleracea var. alboglabra , Brassica oleracea var italica F 1-derived doubled-haploid ( DH ) population with 46 lines .

本研究利用双单倍体群体构建了不结球白菜遗传连锁图谱，并在此基础上进行了主要农艺性状的QTL定位研究。

In this study , several doubled haploid ( DH ) populations of non-heading Chinese cabbage were obtained through isolated microspore culture , and then used for molecular genetic linkage map construction , and mapping QTL for several important agronomic traits .

以7个F1石竹品种和一系列已混杂种子为试材，通过系谱法选育自交系及花药培养选育双单倍体，以期得到符合育种目标的纯系。

In order to get pure lines of Dianthus Chinese for production of F1 seeds that meet the needs of breeding aim . seven commercial F1 hybrid pink cultivars and a series of mixed seeds were used as materials by the pedigree way and anther culture .

高频率诱导马铃薯双单倍体的研究

High frequency of DIHAPLOID induction from the cultivated tetraploid potato

水稻双单倍体群体的分子标记图示基因型分析

Graphical Genotype Analysis of Doubled Haploid Lines of Rice Using Molecular Linkage Map

用小孢子培养创建大白菜双单倍体永久作图群体

Establishing Doubled Haploid Population with Microspore Culture for Genetic Mapping in Chinese Cabbage

青梗菜小孢子植株群体中，单倍体、双单倍体及多倍体同时存在。

Pakchoi microspore plant food groups , haploid , doubled haploid and polyploid exist .

用双单倍体群体构建水稻的分子连锁图

Construction of a Rice Molecular Linkage Map Using a Double Hploid ( DH ) Population

提高马铃薯双单倍体花药培养产生胚状体及再生植株频率的研究

Study on increasing frequency of EMBROIDS and regenerated shoots from DIHAPLOID potato by anther culture in vitro

根据目前马铃薯育种方向，获得马铃薯双单倍体是马铃薯育种成功的关键.就马铃薯双单体细胞学。

According to the present direction , the key to the success for Potato breeding is to obtain double haploid .

双单倍体方法是一项已经广泛应用于小麦遗传育种研究的生物技术。

Doubled haploid ( DH ) system is a biotechnological tool which has been widely applied in wheat breeding programmes .

采用游离小孢子培养双单倍体技术快速育成早熟大白菜杂交一代新品种豫新50。

Yuxin 50 is a new Chinese cabbage F 1 hybrid developed by doubled haploid lines induced by isolated microspore culture .

双单倍体诱导纯合四倍体得到了较理想的结果，加倍频率达16.9%。

A better result has been got in inducing double haploid to pure tetraploid , and the frequency is 16.9 % .

21种基因型小孢子再生植株中双单倍体比率为23.94%～99.42%。

The ratio of doubled haploid plants derived from treated microspores of 21 genotypes ranged from 23 . 94 % to 99 . 42 % .

结果表明，在不同光照条件下，单倍体植株净光合速率均明显比双单倍体低。

The net photosynthetic rate ( Pn ) of haploid plant was lower than that of doubled haploid in any photo flux density ( PFD ) .

根据抗病基因保守区克隆小麦抗病基因及小麦抗赤霉病基因双单倍体作图群体的构建

Molecular Cloning of Disease Resistance Genes Based on the Conserved Domains of Plant Resistance Genes , and Construction of Double Haploid ( DH ) Populations for Mapping Scab Resistance Genes in Wheat

单倍体的自然加倍率较高，无需人工加倍即可获得大量的双单倍体及多倍体，多倍体比例为33.16%，双单倍体化植株比例为51.34%。

Haploid doubling rate of high natural , no artificial double to get a lot of double haploid and polyploid polyploid ratio of 33.16 % , the ratio of double-haploid plants of 51.34 % . 9 .

对染色体加倍方法研究的结果表明，用双单倍体或一倍体试管苗的茎段或叶片诱导愈伤组织然后再分化植株的方法加倍的效果最好。

It was proved that the method of inducing calli from the segments of stems or leaves of test tube dihaploid plants and then regenerating plantlets was the best one for doubling the number of potato chromosomes .

但是由离体小孢子自发染色体加倍形成的双单倍体植株仅占其再生植株群体的10%-30%。约70%-90%的植株是单倍体和嵌合体，不能正常开花产生一定量的种子，难以满足遗传和育种研究的需要。

But the spontaneous chromosome doubling frequency of microspore-derived plants is very low , 70 % to 90 % plants remains haploid , which flower abnormally and can not produce enough seeds , so it can not be utilized in genetic and breeding program research .