图位克隆

利用图位克隆（map-basedcloning）的策略，对拟南芥AST基因进行克隆。

The map-based cloning strategy was used to clone the AST gene .

本文主要应用图位克隆（Map-basedcloning）的方法定位长护颖基因。

This article major in the map-based cloning method to find the long glume gene .

对图位克隆法获得的4个抗病候选基因进行RT-PCR分析，表明4个候选基因在转录水平上均有表达。

Results show that all 4 candidate genes were expressed in mRNA level .

图位克隆表明一个编码SPP的基因发生突变。

The mutated gene was cloned by map-based cloning , and was shown to encode an SPP.

为了进一步精细定位im基因，为图位克隆该基因奠定基础。

In order to lay a good foundation for future map based cloning of the im gene , fine mapping of the im gene was performed .

通过关联分析获得了与棉花重要性状相关的SSCP标记，这为棉花重要性状的图位克隆和分子标记辅助选择育种提供了又一基础。

SSCP markers associated with important agronomic traits by association analysis provide a new foundation for map-based cloning and MAS .

遗传图谱是数量性状基因定位（QTL）、基因图位克隆、比较基因组学研究以及分子标记辅助育种等的基础。

Genetic linkage map is a QTL mapping , the genetic map spaces cloning , comparative genomics research and and molecular marker-assisted breeding the foundation .

这些生长相关性状的QTL及功能基因的定位为我们从事分子标记辅助育种和功能基因的图位克隆提供了参考区间。

The location of functional genes and QTLs associated with the growth would be very useful for molecular marker-assisted selection and map-based cloning of functional genes .

水稻长节间基因Eui的图位克隆及功能分析

Positional Cloning and Functional Study of the Rice Gene Eui Controlling Elongation of Uppermost Internode

次级群体是用于QTL鉴定、精细定位、互作分析、图位克隆、品种改良等的良好材料。

And they were powerful materials for QTL identification , QTL fine mapping , analysis of QTL interaction , map based on clone , variety improvement and so on .

本文综述了野生番茄单片段渐渗系的创建及其在基因精细定位、QTL遗传效应分析以及图位克隆中的应用。

This paper reviews establishment of introgression lines from wild tomato in Lycopersicon by MAS and application of theses lines in fine mapping , analysis of QTL effect and gene map-based cloning .

该图谱将为下一步的重要农艺性状的QTL定位、分子标记辅助育种、目标基因的图位克隆奠定了一定的理论基础。

The molecular linkage map would base a theoretical foundation for the next gene location of important quantity traits , molecular marker assistant breeding , goal gene location and map based cloning .

结合玉米基因组测序公布的BAC信息，利用课题组构建的近等基因系，利用各种方法在抗病基因区域内开发分子标记，并对抗病基因进行定位，为其图位克隆奠定基础。

Combination of maize genome sequencing information , development of molecular markers and mapping of resistance genes by using near isogenic lines in Maize is base of map-based cloning .

在本研究中我们通过图位克隆的方法分离到了PLA1基因。

In this work , we isolated the PLA1 gene by map-based cloning .

准确性高的、确实存在的QTL可以用来做分子标记辅助选择，可以进一步做图位克隆，而假阳性QTL是无意义的。

The existence of a QTL should be of high confidence if it is to be used in MAS or even more in map-based clone while those false positive QTLs are of no sense .

图位克隆结果表明BER15编码一个细胞色素P450单加氧酶，是植物激素油菜素内酯合成途径中的一个关键酶。

Map-based cloning of BER15 demonstrated BER15 encodes a cytochrome P450 monooxygenase which acts as a key enzyme in brassinolide synthesis .

由此作者认为这个微效QTL有着很高的遗传力，可以通过NIL-F2群体将该基因图位克隆。

Therefore , we suggested that minor QTLs for traits with high heritability could be isolated using a map-based cloning strategy in a large NIL-F2 population .

为了研究该QTL的分子基础，我们通过图位克隆的方法分离到了SKC1基因，发现它编码一个HKT型转运蛋白。

To understand the molecular basis of this QTL , we isolated the SKC1 gene by map-based cloning and found that it encoded a member of HKT-type transporters .

利用图位克隆方法，本实验室已确定POS的侯选基因是一个编码C2H2zinc-finger蛋白的转录因子，与拟南芥的JAG基因同源。

Using positional cloning approach , our laboratory had characterized the candidate gene of POS as a transcription factor encoding a C2H2 zinc-finger protein , closely homologous to Arabidopsis JAGGED ( JAG ) .

本研究主要在实验室已有工作的基础上，利用各种方法寻找与ms2紧密连锁的分子标记，为图位克隆ms2奠定基础。

Our study aims at finding the molecular marker tightly linked to ms2 and preparing for map-based cloning ms2 based on the previous work done by our lab.

稻米品质性状的遗传基础研究以及粒长基因GS3的图位克隆

Study on the Genetic Basis of Grain Quality Traits and Map-based Cloning of GS 3 for Grain Length in Rice

为了从分子和遗传水平上理解水稻早衰机理，我们采用图位克隆的策略定位Pse（t）基因。

In order to improve our understanding of the molecular mechanism for premature senescence in rice at the genetic and molecular levels , a map-based cloning strategy was employed to locate the Pse ( t ) gene .

本文，我们图位克隆到一个控制粒宽，结实率和千粒重的数量性状基因GS5。

We showed that the quantitative trait locus ( QTL ) GS5 in rice controls grain size by regulating grain width , filling and weight .

本文综述了BAC的发展，利用此载体构建基因组文库的程序和鉴定方法，及其在物理图谱构建、图位克隆、基因组测序、转基因技术等研究中的应用。

This paper presents a brief introduction of the development of BAC , the way of construction and identification of BAC library , and its applications in genomic physical mapping , genes map-based cloning , genomic sequencing and gene structure and function study .

图位克隆是发现新基因的重要手段。

Positional clone is an important method for discovering novel genes .

图位克隆技术是一种广泛的应用于突变体突变基因定位的一种技术。

Map-based cloning techniques are widely used to identify the mutation gene .

水稻长护颖突变体基因图位克隆

Map-Based Gene C long ing in Rice Long Glume Mutant

图位克隆技术在分离植物基因中的应用

The Application of Map-based Gene Cloning Method on Isolating Genes of Plants

作物数量性状基因图位克隆研究进展

Advances in Map-based Cloning for Crop Quantitative Trait Loci

水稻数量性状和其位点的基因图位克隆研究进展

The Research Progress of Rice QTLs and Gene Map-based Cloning Based on QTLs