大岩桐
- 网络Sinningia;sinningia speciosa;Gloxinia
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大岩桐组培苗移栽技术研究初报
Primary Report on Study in Transplant Technique of Tissue Culture Seedling from Sinningia Speciosa
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采用间接酶联免疫吸附法(EnzymeLinkedImmunosorbentassay,简称ELISA)测定了大岩桐花瓣发育过程中4种内源激素含量的动态变化。
The changes of endogenous hormone content during petal development in Sinningia speciosa were measured by indirect ELISA ( enzyme linked immunosorbent assay ) .
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用PCR法对转化再生植株进行了检测,证实NPTⅡ已经整合到大岩桐的基因组DNA中。
PCR was used to detect transgenic plants in the regenerated shoots .
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农杆菌介导的CFL和MADS-box基因转化大岩桐研究
Studies on Agrobacterium Tumefaciens-mediated Sinningia Speciosa Transformation with CFL and MADS-box Genes
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本研究用野生型、突变型MAPKK基因转化观赏花卉大岩桐,为进一步研究MAPKK基因的功能奠定基础,也为花卉植物的转基因提供实验系统。
The aim of this research is to establish a transgenic system for flower plants and investigate the functions of MAPKK in transgenic plants in the future .
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北方大岩桐组培试管苗瓶外移栽试验
The Research of Transplanting Test Tube Gloxinia Plantlets In The Field
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影响大岩桐试管块茎形成的若干因素
Fators effecting on the formation of test-tube tubers of Sinningia speciosa plantlets
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大岩桐的试管生根诱导与移栽技术研究
Study on Roots Inducement in Tube and Transplant Technique of Sinningia speciosa
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大岩桐开花生物学的观察及快繁技术的研究
Study on Blooming Biological Characteristics and Rapid Propagation Technique of Sinningia speciosa
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大岩桐快繁体系优化研究
Study on Optimized System for Rapid Propagation in vitro of Sinningia speciosa
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以叶盘为外植体,初步建立了大岩桐的遗传转化体系。
A genetic transformation system was established by using leaf discs of Sinningia speciosa .
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利用大岩桐腋芽诱导分化育苗技术研究
Research of the Technique of Cultivating Seedlings Derived by Using Axillary Bud of Gloxinia
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以大岩桐的叶片为外植体进行组织培养。
The tube propagation of gloxinia was studied by using the leaves as the explants .
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不同基质对大岩桐移栽成活率的影响研究
The Research for the Different Matrix 's Impact on the Survival Rate of Transplanting of Gloxinia
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大岩桐的组织培养
Tissue culture of gloxinia
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以大岩桐幼嫩叶片为外殖体,通过0.1%氯化汞消毒后进行组织培养,适宜条件为:(1)诱导培养基为MS+BA2.0mg.L-1(以下单位同)+NAA0.2;
Using sinningia speciosa leaves as explant treated with 0.1 % mercuric chloride , the optimum conditions for tissue culture are : ( 1 ) inducing culture is MS + BA2.0 + NAA0.2 ;
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同时,对大岩桐花瓣发育过程中四种内源激素的动态变化做了定量分析,为进一步研究花器官发育的激素调控机制积累科学素材。
Meanwhile , quantitative analysis on the dynamic changes of four endogenous hormones in petal development of Sinningia speciosa was done , which provide a scientific material for the further studying the mechanism of floral organ development .