层析柱
- chromatography column;chromatographic column
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凝血酶经CM离子交换液相层析柱进一步纯化。
Thrombin was further purified with CM ion-exchange liquid chromatographic column .
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在长径比为25的层析柱上,以正交实验确定最佳的色层分离工艺,其分离度Rf达到0.87。
By chromatographic column of length and diameter ratio 25 , the optical chromatographic technology was determined by positive cross test , and its separating degree Rf is up to 0 . 87 . The testing results were identified by the ion chromatography .
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多粘菌素B琼脂糖层析柱清除体液中内毒素
Removal of endotoxin in various solution by polymyxin b-sepharose 4B affinity chromatography
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抗人C1抑制物单克隆抗体亲和层析柱分离的C1抑制物性质初探
Quality Study of C_1 Inhibitor Isolating from Anti-human C_1 Inhibitor McAb Affinity Chromatography Column
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利用ProteinA层析柱分离、纯化,获得纯度较高的融合抗体。
Through Protein A affinity chromatography , the fusion antibody with high purity and biological activity was obtained .
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单克隆抗体亲和层析柱在测定酱油与乳腐中黄曲霉霉素B1的应用
Application of monoclonal antibody affinity column to measurement of aflatoxin b_1 in soy sauce and fermented bean curd
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融和蛋白经过Ni离子亲和层析柱纯化。
The protein was purified by Ni2 + affinity column .
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Y(x)是溶质在层析柱中的正态分配函数。
Y ( x ) is a regular distribution function of the solute on the chromatogram colume .
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经过Ni离子亲和层析柱纯化后,得到纯度大于85%的蛋白。
The purity coefficient of protein exceeds 85 % after purification by Ni2 + affinity column . 5 .
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用葡聚糖凝胶G-75层析柱制备高纯度细胞色素C
The Preparation of Highly Pure Cytochrome C by Using Sephadex G 75
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用含Ca~(2+)缓冲液清洗层析柱后(洗脱物Pc为另一重要产品),该柱可反复使用。
The affinity chromatographic column could be washed with a buffer containing Ca2 + and the PC eluted product could be reused .
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采用谷眺甘肽亲和层析柱纯化GST融合蛋白,经肠激酶特异性裂解释放出目的蛋白。
The GST fusion protein was purified by glutathione affinity chromatography .
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将血清直接上样DSA亲和层析柱,分离肝型碱性磷酸酶强结合组分。
The sera were chromatographed directly on the DSA lectin column to isolate the strongly binding fraction .
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方法常规方法免疫BALB/c小鼠制备腹水,应用G蛋白亲和层析柱对小鼠腹水样品进行分离纯化。
Methods BALB / c mice were immunized by routine technique to prepare ascites , and the ascites samples were purified using protein G affinity chromatography column .
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◆通过蛋白A琼脂糖亲和层析柱制备和纯化血浆IgG,采用分光光度法对纯化的IgG进行浓度的测定。
◆ IgG are prepared and purified by protein A agarose affinity chromatography column and the concentration are detected by spectrophotometry .
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进行了5′AMPQT4亲和层析柱纯化乳酸脱氢酶(LDH)的试验。
An affinity column based on 5 ' - AMP-QT_4 was applied to purify lactate dehydrogenase ( LDH ) .
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ColiBL21(DE3)中表达,镍亲和层析柱纯化。
Coli BL21 ( DE3 ) . The fusion protein was purified through Nickel-affinity chromatography column .
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该多糖经SephadexG&200凝胶层析柱和高压电泳分析证明是单一成份。
The polysaccharide gave a single peak on gel chromatography with Sephadex G-200 and high voltage electrophoresis .
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表达的蛋白用Ni~(2+)亲和层析柱纯化后做SDS-PAGE检测,以及蛋白印迹(westernblot,Wb)和间接ELISA实验的应用研究。
The expressed Gag antigen was detected by SDS-PAGE , Western blot ( Wb ) and ELISA after purified by Ni2 + - NTA affinity chromatography .
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番茄成熟果实经抽提上鸡卵类粘蛋白(OM)-Sepharose4B亲和层析柱分离纯化制得番茄凝集素。
A lectin from the edible tomato ( Lycopersicon esculentum ) was purified by hen ovomucoid-Sepharose 4B affinity chromatography .
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通过NiNTA层析柱对重组蛋白进行纯化。
The recombinant protein was purified with Ni-NTA agarose bead column , and its bioactivity was determined with hyaluronan binding assay .
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所得粗肽用SP-Sepharose-FF层析柱和半制备型HPLC分离纯化。
Then purified with SP-Sepharose-FF and semi-preparative HPLC .
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用Ni2+-His亲和层析柱对包涵体蛋白进行纯化,纯化后的蛋白经SDS-PAGE电泳检测呈现单一条带。
A sigle band appeared for the purified protein by SDS-PAGE after the fusion proteins were purified by Ni2 + - His affinity columns .
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利用Ni亲和层析柱将表达产物进行了纯化,纯化效率达95%以上,再利用分步透析进行了纯化蛋白的成功复性。
The expression product was purified by affinity chromatography with Ni-NTA agarose , and efficiency of purification was more than 95 % . The purified protein was successfully refolded by gradient dialysis .
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应用有机汞亲和层析柱分离经微球菌核酸酶有限制消化的HL-60细胞核,得到有机汞层析柱亲和及不亲和的2个核小体组分。
The unbound and bound fractions of nucleosome from HL-60 cell nuclei digested with micrococcus nuclease were separated by Hg-affinity chromatographic column .
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结果:用抗scFvmAb亲和层析柱纯化的重组人源性抗HBsAgFab的纯度可达95%以上,回收率可达70%~85%。
RESULTS : The purity of purified recombinant anti-HBsAg Fab was above 95 % and its recovery rate was about 75 % - 85 % .
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结果显示,在薄层硅胶H为填充剂的干层析柱上,以石油醚-乙酸乙酯(15∶1)为洗脱剂,可以将大黄酚和大黄素甲醚分离。
The results indicated that they could be separated through silica gel H for thin layer chromatography as the stational phase , and petroleum aether and ethyl acetate ( 15 ∶ 1 ) as the mobile phase .
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通过索氏提取气溶胶样品,抽提物经硅胶层析柱分离,使用16种多环芳烃混合标准样品绘制标准曲线,以外标法对PAHs进行定量分析,并根据所得数据浅析了多环芳烃污染来源。
Coupled with soxhlet extraction and silicagel column chromatographic separation techniques . The method of mixed standard curves were used to quantitatively determine 16 PAHs in aerosol samples .
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本实验以DNA亲和层析柱为手段分离提取Ehrlich腹水癌及各种非癌疾患小鼠的血清DNA结合蛋白(DBP),并采用了SDS-聚丙烯酰胺凝胶电泳法对之进行了比较分析。
The DNA binding proteins ( DBPs ) in the serum of mice with Ehrlich ascites carcinoma or various non-malignant diseases were separated by DNA-cellulose column and analyzed by SDS-polyacrylamide gel electro-phoresis .
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用自制的免疫亲和层析柱用于转基因羊乳中rhEPO的提纯,具有很好的吸附作用,回收率达70%。
The immunoaffinity chromatography column could adsorb 70 % of rhEPO in purifying the rhEPO from transgenic goat milk .