拷贝数
- 名copy number
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增生性瘢痕与瘢痕疙瘩DNA拷贝数变化的差异分析
Differences of DNA copy number changes between hyperplastic scar and keloid
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喉癌线粒体DNA拷贝数的临床意义
Clinical significance of increase in mitochondrial DNA copy number in laryngeal squamous cell carcinoma
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应用引物延伸预扩增提高微量DNA拷贝数
To increase copy of small DNA sample by primer-extension preamplification
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乳腺癌线粒体DNA拷贝数改变的研究及意义
Study on copy numbers alteration of mitochondrial DNA in breast cancer tissue
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肺鳞癌转移相关基因组DNA拷贝数改变的研究
A Study on DNA Copy Number Alterations Related to Metastasis in Lung Squamous Cell Carcinomas
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最后对本研究中用于进行病毒绝对拷贝数定量的实时荧光定量PCR的技术原理和应用前景进行了简要介绍。
The principle of mechanism of Real-Time Quantitative PCR is introduced .
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方法用PCR定量法检测血清中HBVdna拷贝数,以及与转氨酶的相关性。
Methods HBV-DNA copies in patients were detected by PCR quantitative method .
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病例3和病例5其基因组DNA分别合并有重复和缺失的复杂DNA拷贝数变异。
Case 3 and case 5 : There are the cases of complex genomic duplications and deletions .
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起始拷贝数越多,Ct值越小。
The more number the template onset copy has , and less the Ct value will be .
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用实时定量PCR检测标本线粒体DNA的拷贝数。
Moreover , To detect the mitochondria DNA copy numbers of sample with real-time PCR .
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利用实时荧光定量PCR方法分析转基因水稻外源基因拷贝数
Estimating copy number of transgenes in transformed rice by real time quantitative PCR
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结论:线粒体DNA拷贝数改变与乳腺癌发生有关。
Conclusion : It is relative that copy numbers alteration of mitochondria DNA with generation of breast cancer .
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从病毒悬液中提取DNA,采用实时荧光定量PCR检测病毒基因拷贝数。
Extracted DNA from virion suspension and quantified viral genomes copies by fluorescence quantitated real-time PCR ;
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转基因马铃薯植株中外源基因PCR扩增和拷贝数测定
PCR Amplification and Copy Number Analysis of Foreign Gene in Transgenic Potato Plants Hasi Agula
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一种能精确检测人间期细胞核中21号染色体拷贝数的DNA探针
A DNA Probe Suitable for the Detection of Chromosome 21 Copy Number in Human Interphase Nuclei by Fluorescence in Situ Hybridization
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实时荧光定量PCR检测毕赤酵母基因组中外源基因拷贝数
Determination of Copy Number of Foreign Gene in Genome of Pichia pastoris by Real-time Fluorescent Quantitative PCR
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用荧光定量PCR方法检测转染细胞中外源基因的拷贝数
Detection of the Copy Numbers of Exogenous Gene of Transfected Cells by Fluorescent Quantitative Polymerase Chain Reaction
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荧光定量PCR检测细菌染色体上基因拷贝数
The copy number of the gene in chromosome of strain detected by the real time quantitative PCR
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Southern杂交分析显示大部分株系呈阳性,拷贝数1-4。
Southern blot analysis indicated that most transgenic lines are positive and gene copy number ranged frome 1 to 4.2 .
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荧光定量PCR法检测重组汉逊酵母中HBsAg基因的拷贝数
Estimation of the HBsAg Gene Copy Number of Recombinant Hansenula Polymorpha Yeast by Fluorescence Quantitative PCR
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Southern杂交结果显示,盐藻烯醇酶基因已整合到植物基因组中,在转基因烟草中的拷贝数为2~4个。
The Southern hybridization results showed there were 2 ~ 4 copies of Dunaliella salina enolase gene in the transgenic tobacco .
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应用实时定量PCR,检测秀丽线虫的线粒体拷贝数(mtDNA)。
The mtDNA copy number of C. elegans was determined with the quantitative PCR .
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本研究以细菌染色体之外的遗传因子&质粒为对象,研究La3+对质粒DNA复制拷贝数的影响。
So the genetic factor-plasmid is used as experimental material to research the effect of La (ⅲ) on replication copy number of plasmid DNA .
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实时定量PCR方法检测鼻咽癌病人全血EB病毒拷贝数的实验研究
Real-time quantitative PCR assay of Epstein-Barr virus in whole blood cells of nasopharyngeal carcinoma patients
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Southern杂交分析表明若处于很强的选择压力下,该质粒的拷贝数会发生有效的扩增。
By means of southern hybridization analysis , the efficient amplification of plasmid copy number was detected under a strong selection pressure .
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检测上清PGE2分泌、细胞增殖和细胞PPAR-γmRNA拷贝数。
Cell proliferation , PGE_2 secretion and PPAR - γ mRNA were detected .
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对部分PCR阳性株进行Southern杂交检测,证明外源基因已经整合到植株基因组中,而且拷贝数较低,为1-2个拷贝。
Southern blot analysis of part of positive transgenic plants revealed that the exogenous gene has been integrated into the plant genome .
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病毒RNA分子拷贝数和靶基因mRNA水平用实时定量RT-PCR确定。
The RNA copies of viral vectors and mRNA levels of target genes were determined by quantitative real-time RT-PCR .
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而亲本Ds元件拷贝数对Ds的跳跃频率影响不大。
It was found that Ds copy number had little influence on Ds transposition frequency .
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经检验,每个PCR反应孔的理论cDNA模板拷贝数为0.44个,说明扩增反应是特异有效的。
Upon examination , each PCR reaction contains theoretical 0.44 copy number of cDNA template , indicating that amplification was specific and effective .