李斯特菌

多重-巢式PCR检测食品中单增李斯特菌研究

Rapid Detection of Listeria Monocytogenes in Food with Multiplex - nested PCR

荧光实时定量PCR检测单核李斯特菌方法学建立及应用

Development and Application of Fluorescence Real-time Quantitative PCR for Detecting Listeria Monocytogenes

用随机扩增DNA多态性制备李斯特菌属特异探针

RAPD-PCR in isolating a generic specific DNA probe for listeria

PCR方法检测单核细胞增多性李斯特菌的实验研究

Detection of Listeria monocytogenes in MJME by PCR technique

改良分子信标-实时PCR快速检测产单核李斯特菌

Rapid simultaneous detection of Listeria monocytogenes using modified molecular beacons and real-time PCR

PCR在快速检测食品单核细胞增多性李斯特菌中的应用

Application of Polymerase Chain Reaction on Rapid Detection of Listeria monocytogenes in Food

荧光定量PCR技术用于模拟临床标本单增李斯特菌检测的研究

Real-time PCR-based method for the detection of Listeria monocytogenes in simulated clinical sample

单增李斯特菌与志贺氏菌多重PCR检测技术的建立

The Development of Multiplex PCR Technique for Detection of Listeria Monocytogenes and Shigella

食品中产单核李斯特菌PCR检测灵敏度的研究

Study on sensitivity of PCR method for the detection of Listeria monocytogenes in food

应用PCR方法检定单核细胞增多性李斯特菌

Identification of Listeria monocytogenes by PCR Method

李斯特菌DNA对小鼠H（22）肝癌Bcl-2基因表达的影响

Effect of Listeria DNA on Expression of Bcl-2 in H_ ( 22 ) Hepatocarcinoma in Mice

目的：研究PCR检测不同食品中产单核李斯特菌的灵敏度。

Objective : To study the sensitivity of PCR method for the detection of Listeria monocytogenes in food .

3MPetrifilm~（TM）环境李斯特菌测试片的比较实验

Comparison Experiment of 3M Petrifilm ~ ( TM ) Environmental Listeria Plate

目的单核细胞增生李斯特菌（Listeriamonocytogenes，Lm）属于革兰阳性无芽孢杆菌李斯特菌属，主要通过食物传播。

Objective Listeria monocytogenes is a gram-positive , no spore , food-borne pathogen which causes listeriosis .

单增李斯特菌PCR-ELISA快速检测技术研究

Study on rapid detection techniques of PCR-ELISA for Listeria monocytogenes

本研究发现了一些潜在的李斯特菌毒力因子，为LM与LI的致病性差异研究提供了新的方向。

This study found a set of potential Listeria virulence factors , pointed out a new direction for research on the pathogenic difference between LM and LI .

基于ActA基因的单核细胞增多性李斯特菌的序列分型研究

ActA gene sequence typing of Listeria monocytogenes

总之，本试验所建立的三重PCR和RAPD分型方法为进一步开展牛奶生产加工过程中李斯特菌污染的监控奠定了基础。

In conclusion , the present studies on triplex PCR identification and RAPD typing have provided good means for further work on the control of the listerial contamination in pasteurized milk .

[方法]对经传统方法结合ATB鉴定分离的菌株（单核细胞增生李斯特菌）分别采用accuprobe和PCR试剂盒进行鉴定比较。

[ Methods ] Tradtional identification method were compared with molecular method ( accuprobe and PCR ) .

SPA-ELISA用于单核细胞增生性李斯特菌检验的应用研究

Study on application of SPA-ELISA for detection of Listeria Monocytogenes

这些结果表明3M测试片用于食品加工环境李斯特菌的快速检测不失为一种好方法。

We can conclude that the3M Petrifilm is an alternative rapid method for detecting Listeria species in environmental samples from food plant .

基于actA基因的产单核细胞李斯特菌遗传谱系研究

Lineage classification of Chinese Listeria monocytogenes isolates based on the partial sequence of the actA gene

GFP用于单核细胞增生李斯特菌PrfA调控毒力基因actA转录表达的研究

Use of GFP to Study the Expression of the Virulence Gene actA Regulated by PrfA in Listeria monocytogenes

李斯特菌属包括七个种，其中单核细胞增多性李斯特菌（Listeriamonocytogenes，以下简称Lm或单增李斯特菌）是能引起人畜共患病的食源性致病菌。

The genus Listeria consists of seven species , one of which named Listeria monocytogenes , short for Lm , is a zoonosis pathogen .

单核细胞增生李斯特菌毒力基因actA转录调控机制的初步研究和缺失突变株的构建

The Construction of an Attenuated Strain and Research on Expression of the Virulence Gene actA Regulated by PrfA in Listeria Monocytogenes

多抗的ELISA检测结果显示制备所得多抗对副溶血性弧菌具有较强的特异性反应，同时对单增李斯特菌、金黄色葡萄球菌、铜绿假单胞菌也有较弱的交叉反应。

It was found that the polyclonal antibody can well identify Vibrio parahaemolyticus , while it has the weak response to Listeria monocytogenes , Staphylococcus aureus , Pseudomonas aeruginosa and an unknown strain of Vibrio by the ELISA detection . 4 .

药敏试验结果显示，沙门菌、副溶血性弧菌、O157∶H7大肠杆菌均有多重耐药株，单核细胞增生性李斯特菌对抗生素产生耐药性的比例较低。

Antibiotic susceptibility tests revealed that Salmonella , Vibrio parahaemolyticus and EHEC O157 ∶ H7 had multiple antibiotic resistant strains , but most Listeria monocytogenes strains showed high antibiotic susceptibility .

根据单核细胞增多性李斯特菌ActA基因序列保守区设计一对引物，对21个菌株进行PCR扩增，得到长度为820bp的片段。

Using ActA-based DNA sequence typing procedure , a pair of primers was designed for PCR amplification of a 820 bp DNA fragment . The products from 21 Listeria monocytogenes strains were submitted directly for sequencing .

用聚合酶链式反应（PCR）扩增hly基因，检测单核细胞增生症李斯特菌的纯培养物及其模拟污染的生猪肉、水和牛奶。

A polymerase chain reaction ( PCR ) assay targeting the gene encoding hly was developed for detecting Listeria monocytogenes in pure cell cultures and on artificially contaminated water , milk and pork .

目的研究转录调控蛋白PrfA对两组新近发现的单核细胞增生李斯特菌基因的体外转录作用。

Objective The aim of study was to investigate PrfA-dependent transcription activities of two groups of newly identified and putatively in vivo PrfA-regulated genes of listeria monocytogenes .