染色细胞

TUNEL阳性染色细胞随时间延长而增多。

Positive cells of TUNEL staining increased in the course of time .

超短波对兔内毒素急性肺损伤肺内NOS阳性染色细胞数量的影响

Effects of ultrashort wave on numbers of pulmonary positive nitric oxide synthase staining cells in lung of the rabbits with acute endotoxin-induced lung injury

CD31免疫荧光染色细胞表面呈现黄绿色荧光。

CD 31 antigens cells showed green fluorescence .

结果伤后2,4,8,12周，A组S-100、MBP染色细胞和ISH细胞差异无统计学意义（P>0.05），其中ISH阳性细胞可见髓鞘形成。

Results There was no significant difference among staining positive cells , S 100 MBP and positive ISH cells in Group A.

PI染色细胞可以显示分布于G0-1，S，G2-M期以及细胞凋亡情况。

The PI stained cells revealed their distributions in the G0-1 , S and G2-M phases and the incidence of apoptosis .

方法采用电镜、还原型辅酶&黄递酶（NADPH-d）组织化学技术观察兔内毒素性急性肺损伤时肺组织超微结构的改变和肺内小血管、细小支气管、肺泡的NOS阳性染色细胞数目的变化。

Methods NADPH d histochemical technique was used to observe the changes in the ultrastructure of lung tissue and NOS positive staining cells in small vessels , bronchioles and pulmonary alveoli of rabbits with endotoxin induced acute pulmonary injury .

于24h、48h、72h，对各组细胞进行台盼蓝染色细胞计数并绘制生长曲线。

Trypan blue dyeing and cell number counting : BT-325 cells excluding from trypan blue dyeing after 24 , 48 or 72h of treatment for cells , were counted in the cell counter to draw the growth curve of cells after 72h .

大型不染色细胞在上呼吸道感染中的临床意义

Clinical significance of large unstained cells in upper respiratory infection

方法：活细胞显微摄影、伊红染色细胞计数、绘制细胞生长曲线方法。

Methods : Micrography of Living Cells . Diagram of Cellular Growth .

结果：随着传代次数增加，油红O染色细胞计数值与光密度值均下降。

Results The value of cell count by Oil red O stain coloration and optical density declined as the passages increased .

激光共聚焦显微镜下显示雪旺细胞、神经元、内皮细胞模型清晰可见，雪旺细胞为蓝色染色细胞，细胞呈长梭形，胞核呈卵圆形或长圆形，海鱼状排列生长。

In the laser scanning confocal microscope , schwann cells are long spindle cell , its nucleus are ovoid or oblong . schwann cells are fish shape arrangement .

图像分析测定单位面积内焦油紫染色细胞面积总和，各实验组两侧间均有显著性或极显著性差异（P<0.05，P<0.01）；

The areas of cresyl violet steined cells between the denervated and intact striatum were significant difference in every experimental group ( P < 0.05 , P < 0.01 ) .

各向异性导电胶中柔性颗粒化学镀镍研究结果表皮生长因子阳性染色细胞主要分布于棘层和颗粒层。

The Research of the Electroless Plating on the Soft Microspheres Applied in the ACA ; The results showed that the EGF positive staining cells were mainly localized in the stratum spinosum and stratum granulosum .

通过流式细胞仪检测PI染色的细胞DNA，发现病毒感染组出现明显凋亡峰；

Flow cytometric observation on DNA of PI stained cells showed obviously an apoptotic peak in the Cox B infected group .

方法：体外试验MTT法测存活率，甲基绿-派诺宁联合染色观察细胞凋亡形态，流式细胞术、琼脂糖凝胶电泳观察DNA损伤及细胞周期。

Methods : MTT , methyl green and pyronin stain assay , flow cytometry , agarose gel electrophoresis were used .

肠组织TUNEL染色检测细胞凋亡；

The apoptosis in intestine was detected by TUNEL staining .

免疫细胞化学染色检测细胞增殖核抗原（PCNA）的表达；

The expression of proliferating cell nuclear antigen ( PCNA ) was assessed by immunocytochemistry method .

PCNA免疫组化染色观察细胞的增殖；

Cell proliferation with PCNA immunohistochemistry ;

MTT方法检测细胞活力，免疫荧光细胞化学染色观察细胞内包涵体形成的变化。

Cell vitality was measured by MTT assay . The formation of inclusions was determined with immunofluorescence cytochemistry staining .

Cp对7种细胞系活性没有影响，并且Hoechst染色和细胞成集落实验显示对7种细胞没有明显作用。

And Hoechst staining and colony-forming unit had no obvious effect onthe seven kinds of cell lines .

当油酸作用肝细胞24h、48h、72h后采用油红O染色观察细胞内的脂滴的积聚情况。

The accumulation of lipid droplets in hepatocytes were observed by light microscopy after oil red staining .

用RT-PCR、Western-blot、免疫荧光染色以及细胞计数的方法，观测神经元的分化情况与神经干细胞及Notch通路信号分子表达的关系。

RT-PCR , Western-blot , immunocytochemistry and cell counting were employed to observe the neuron differentiation and Notch pathway molecules .

Hoechst染色检测细胞凋亡。

Hoechst staining was applied to examine apoptosis of LEC .

ET-1免疫组化染色阳性细胞主要分布在肺动脉血管壁和心肌细胞上，染色程度由强到弱依次为B组>C组>A组。

The distribution of ET-1 positive cells was seen in pulmonary arterial wall and cardiomyocytes . The ET-1 immunoreactivity was group B > group C > group A by turns .

应用Olympus光学显微镜观察细胞抗-神经巢蛋白和D2受体阳性染色阳性细胞数目计数。

The number of positive cells of cell anti-nerve nidogen and D2 receptor positive staining with Olympus optical microscope .

采用四唑盐（MTT）比色法测定细胞活性、吖啶橙荧光染色观察细胞凋亡的形态学变化，WESTERNBLOTTING分析Bcl-2、Bax和caspase-3蛋白的表达。

Cell viability was determined by MTT assay , apoptosis by acridine orange fluorescence dyeing , and expressions of Bcl-2 、 Bax and caspase-3 were analyzed by Western blotting .

方法：采用MTT法计算细胞存活率、Giemsa染色观察细胞形态变化及凋亡情况、流式细胞术检测细胞周期变化。

Methods : The cell growth inhibition , apoptosis and cell cycle changes were detected by MTT test , Giemsa staining and flow cytometry respectively .

还用台盼蓝拒染法确定细胞活力以及培养AML细胞离心涂片细胞化学染色观察细胞形态。

The viability of cultured AML cells was also evaluated by Trypan blue exclusion . Cytospin specimens of cultured AML cells were studied by cytochemical staining .

红油O染色观察细胞内脂质的含量；RT-PCR检测LDL受体（LDLr）的mRNA表达水平。

Intracellular lipid accumulation was assessed with Red Oil O staining , and the expressive levels of mRNA of LDL receptor ( LDLr ) were assessed with RT - PCR .

第4代细胞中进入生长期的细胞约占88.2%；免疫细胞化学染色显示细胞胞质中ALB、CK19染色阳性。

It was 88.2 % in the growing period of 4th generation cells and the stains of ALB and CK19 were positive in Immunocytochemical .