爪蟾
- 网络Xenopus;xenopus laevis;Xenopus tropicalis
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这项研究表明在胚胎发育的过程中,dna并未发生改变,至少对于非洲爪蟾来说是这样的。
This showed that DNA is not altered during embryonic development , at least in Xenopus .
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非洲爪蟾卵母细胞GABAB和GABAC受体介导的电流反应
Current responses mediated by endogenous GABA_B and GABA_C receptors in Xenopus oocytes
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注射热损伤大鼠纹状体mRNA的爪蟾卵母细胞对多巴胺的反应
Responses induced by dopamine in Xenopus laevis oocytes injected with mRNA from heat injured rat striatum
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注射羊外周血淋巴细胞mRNA的爪蟾卵母细胞M型乙酰胆碱电流基本特性
Features of ion current induced by acetylcholine in Xenopus oocytes injected with sheep blood lymphocyte mRNA
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以LambdaDNA为外源性DNA,爪蟾卵非细胞系统中进行核组装。
By adding exogenous Lambda DNA to a cell-free system prepared form Xenopus eggs , nuclei were assembled .
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TGFβ相关蛋白在爪蟾早期胚胎中的免疫组织化学的研究
Immunohistochemical studies on the tgf_ β - related protein in the early development of Xenopus laevis
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微量注射抗TGFβ-1抗体对爪蟾发育的影响
The effect of microinjection of anti-tgf_ β - 1 antibodies on the early development of Xenopus laevis
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方法微量注射仪将纯化的大鼠脑mRNA注入准备好的爪蟾卵母细胞内并于19℃温浴48h以上使其表达;
Methods mRNA was isolated from brain of neonatal rat and injected into prepared Xenopus oocytes .
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GABAA受体在爪蟾卵母细胞的表达
Brain GABA_A receptors expression in Xenopus oocytes in rats
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结论:非洲爪蟾VEGF疫苗治疗可诱导纤维肉瘤小鼠产生抗自身VEGF免疫反应及抗肿瘤作用。
Conclusion : Immunotherapy with xVEGF as a xenogeneic vaccine induces antitumor effect in mice by inducing immune response against self-VEGF .
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外源DNA或染色质在非洲爪蟾卵提取物中可以诱导细胞核样结构的重建。重建核除不具有核仁样结构外,在其它形态结构上与真核细胞核十分相似。
Nuclei assembled in Xenopus egg extracts from exogenous DNA or chromatin are morphologically similar to normal eukaryotic nuclei except that they do not contain nucleolus - like structure .
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以PVS沉淀法和荧光免疫检测法,考察爪蟾V、V工期卵母细胞是否有内源性的LDLR。
㏕ o investigate whether Xenopus Oocyte have endogenetic LDLR with PVS Sedimentation and Fluorescence detection .
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人胎肝中肝细胞生长因子poly(A)~+mRNA在非洲爪蟾卵母细胞内的翻译
Translation of poly ( a ) ~ + mRNA encoding hepatic growth factor derived from human fetal liver in Xenopus laevis oocytes
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鲫鱼视网膜谷氨酸受体和GABA受体在爪蟾卵母细胞中的表达
Studies on glutamate and GABA receptor expressed in amphibian oocytes after injection of carp retina mRNA
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爪蟾卵母细胞中PPARγ-PPRE调控系统的建立和应用
Studies on a PPAR γ - PPRE regulatory systerm in xenopus oocyte and its application
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非洲爪蟾卵母细胞内源性ATP激活电流I(D2)成分的分析
Analysis of the I_ ( D2 ) Component of Endogenous ATP-Induced Inward Currents in Follicular Oocytes of Xenopus Laevis
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Zn~(2+)对爪蟾卵母细胞表达鲫鱼脑GABA受体的调制作用
Zinc modulation of GABA receptors expressed in Xenopus oocytes injected with carp brain poly ( a ) ~ + RNA
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结论①爪蟾V、VI期卵母细胞未见内源性LDLR,不存在对外源人类LDLR基因表达的干扰作用。
Conclusion ( 1 ) There are not endogenetic LDLR on membrane of Xenopus Oocyte of phase V , VI.
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功能性SDF-1受体在非洲爪蟾胚胎发育中的表达研究
Expression of functional receptor of SDF-1 in Xenopus laevis embryo
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采用显微注射法,将所提取的RNA注射于成熟的爪蟾卵母细胞中,采用电压钳位方法记录表达到非洲爪蟾卵母细胞膜上的神经递质受体通道电流。
The mature oocytes of clawed frog were injected with the extracted RNA by microinjection , and double electrode voltage clamps were used to detect activated peak currents of expressed neurotransmitter receptor channel .
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观察淀粉样β蛋白1-40(Aβ1-40)对爪蟾卵母细胞表达的淋巴细胞神经递质受体(NR)功能的影响,探讨淋巴细胞NR功能检测在阿尔茨海默痴呆(AD)病理生理学上的意义。
It is designed to investigate the effects of amyloid β protein ( A β 1-40 ) on the functions of expressed lymphocyte neurotransmitter receptors ( NRs ) in Xenopus oocytes .
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非洲爪蟾GATA-1转录因子与爪蟾发育
Transcription factor GATA-1 of Xenopus and development of Xenopus AFRICA
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作为胚胎发育时期的重要蛋白,头蛋白(noggin)对爪蟾(xenopus)和哺乳动物的中枢神经系统有诱导分化的作用。
As the important gene of embryonic development , noggin has been identified to be the neural inducer of Xenopus and mammalian .
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采用全细胞记录膜片钳技术,研究非洲爪蟾脑片视顶盖神经元微突触后电流(miniatureinhibitorypostsynapticcurrent,mIPSC)频率和振幅对电压依赖关系。
Experiments were performed to study the voltage-dependence of miniature inhibitory postsynaptic current ( mIPSC ) frequency and amplitude using patch-clamp technique with whole cell recording in optic tectal slices of Xenopus .
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它在爪蟾和小鼠的胚胎以及成体小鼠和人中有着广泛的表达,并能与多种LIM同源域蛋白发生相互作用,进而在神经系统发育及红细胞和淋巴细胞的成熟过程中发挥作用。
They can interact with several LIM homeodomain proteins , thereby having important functions in the development of nervous system and the maturation of erythrocyte and lymphocyte .
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在非洲爪蟾和小鼠卵母细胞中,90kDa的核糖体S6蛋白激酶p90rsk是目前研究得最清楚的MAPK底物。
The 90 kDa ribosome S6 protein kinase ( p90rsk ) is the best known MAPK substrate in Xenopus and mouse oocytes .
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研究H+对非洲爪蟾卵母细胞内源性ATP受体的调制作用。采用双极电压钳技术记录卵母细胞膜ATP受体介导的电流。
To investigate the modulatory effect of H ~ + on ATP-activated current and the allosteric modulation through ATP receptor , double electrode voltage clamp technique was used to record the ATP-activated current in healthy Xenopus oocytes .
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结论:成功建立了爪蟾卵母细胞表达体系,并使用双电极电压钳记录技术,对爪蟾卵母细胞表达的NMDA受体不同亚型的药理学特性进行了研究。
Conclusions : The expression system of Xenopus laevis oocytes was successfully established . Using two-electrode voltage clamp recording technique to study about pharmacological properties of expressed different sub-types of NMDA receptors .
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结果测序结果表明,成功地将弓形虫主要表面抗原1单链抗体S1与爪蟾抗菌肽的融合基因构建到原核表达载体pET-32c中;
Results The fused gene of S1 and magainin was successfully cloned into procaryotic expression vector pET-32c proved by DNA sequencing .
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通过与NCBI上已经发表的同源物种进行序列对比,确定测的基因序列是棘胸蛙MHCIIB基因序列,其与非洲爪蟾的相似性达到72%。
Comparison to homologous species which has been published on the NCBI show that the gene sequence obtained is MHC IIB gene sequences and72 % similarity with Xenopus .