特异性结合

转录因子是控制基因表达第一阶段即DNA转录成RNA的一种蛋白质。它通过特异性结合调控区域的DNA序列来调控基因转录过程[1]。

Transcription factors ( TF ) are proteins that control the first step of gene expression , the transcription of DNA into RNA sequences , which regulate gene transcription by specific binding of the regulatory regions of DNA sequences .

对于CHO/CCR5,10μg/mlSaponin对受体G蛋白与[35S]GTPγS的特异性结合无影响，刺激比率大小未变；

On CHO / CCR5 , 10 μ g / ml of Saponin had no effect on the activation of the receptor , the specific binding of [ ~ 35 S ] GTP γ S to receptor G-protein and the stimulation ratio ;

DNA免疫激活序列特异性结合蛋白的研究

Identification of Immunostimulatory Sequence-specific DNA-binding Proteins

首先他们分离与DNA的启动序列特异性结合的转录因子。

First , they isolated transcription factors that were specifically bound to DNA at their promoter sites .

HeLa细胞染色体端粒DNA与核骨架的特异性结合

Specific Attachment of Telomeric DNA to Nuclear Matrix in Hela Cells

端粒存在于真核生物染色体末端，由特定的DNA重复序列及与之特异性结合的蛋白质构成。

Telomeres were composed of specific DNA repeat sequence and associated specific binding proteins in eukaryotes chromosome ends .

EB病毒DNA酶基因特异性结合蛋白的检测

Detection of proteins specifically binding to Epstein Barr virus DNase gene

将获得的抗体进行westernblot分析表明多克隆抗体能与重组蛋白发生很好的特异性结合。

Western blot analysis indicated that the polyclonal antibody could react with the target protein .

FATS基因启动子DNA能与p53转录因子发生特异性结合。

The transcription factor p53 specifically bind to the promoter of FATS gene . 3 .

新型阿片受体配基特异性结合μ受体并抑制cAMP生成

Specific binding to μ opioid receptors by new opioid ligands with inhibition of I cAMP production

westernblot检测结果表明，原核系统表达的E蛋白可以与猪乙型脑炎阳性血清抗体特异性结合。

Western blot analysis showed that the expressed envelope protein from prokaryotic expression can specifically combine with positive serum antibody .

金黄色葡萄球菌蛋白A（staphylococcalproteinA，SPA）是金黄色葡萄球菌的细胞壁蛋白，具有与免疫球蛋白特异性结合的能力。

Staphylococcal protein A ( SPA ) is a cell wall protein of Staphylococcus aureus .

Western印迹分析表明，该表达产物可与HBV感染患者体内的抗X抗体特异性结合。

Western blotting showed that the HX protein could specifically be recognized by the human antibodies against HBV X antigen ( ptotein ) .

SDSpage电泳和westernblot法证实有完整免疫球蛋白的表达，免疫印迹法证实它们能与流感病毒血凝素蛋白特异性结合。

SDS-PAGE and Western blot proved the intact IgG had been expressed and immunoblotting identified they could bind specifically with influenza virus HA .

应用酵母单杂交系统筛选CpG免疫激活序列的特异性结合蛋白

Screening out CpG immunostimulatory sequence-specific DNA-binding proteins with Yeast-one-hybrid system

目的：用黑素瘤（MM）细胞系筛选大容量人源噬菌体抗体库，获得能与MM细胞特异性结合的人源性单链抗体（scFv）。

AIM : To obtain the specific human scFv against malignant melanoma ( MM ) using phage antibody library technology .

结论：目前认为原发性系统性淀粉样变性并发凝血因子X缺乏的机制是淀粉样纤维与X因子特异性结合沉积在组织中形成的。

Conclusion : The mechanism of factor-X deficiency with primary amyloidosis was known as that the factor-X directly bind to amyloid fibrils and deposit in tissue .

ELISA结果显示，噬菌体展示CD28可与抗CD28抗体特异性结合，也可与CD80特异性结合；

ELISA detection showed that phage-displayed CD28 could bind to CD28Ab and CD80 ;

EB病毒LMP1CCT蛋白特异性结合短肽的筛选

Screening and Identification of EBV LMP1 CCT Binding Peptide from Random Phage Display Peptide Library

第二，Rb蛋白可以通过特异性结合肿瘤发生相关的蛋白抑制肿瘤细胞活性。

Secondly , Rb protein can inhibit tumor cell activity by specifically combining with tumor-related proteins .

转化生长因子β（transforminggrowthfactor-，βTGF-β）可通过特异性结合并激活具有丝氨酸/苏氨酸激酶活性的细胞表面受体启动各种应答。

Transforming growth factor β( TGF - β) initiates its diverse cellular responses by binding to and activating specific cell surface receptors that have intrinsic serine / threonine kinase activity .

IgG通过与相应抗原发生特异性结合而发挥免疫效应，是构成机体体液免疫的主要物质。

IgG , the main compositional material of the humoral immune , has the immunologic function when combined with specific antigen .

IFNα2b与肿瘤新生血管特异性结合多肽融合蛋白基因的克隆、表达及表达产物的纯化、鉴定

Cloning and Expression of Tumour Vascular Targeted IFN-IFN α 2b-NGR , Fusion Protein Purification and Its Preliminary Identification

人γδT细胞TCR分子与结核杆菌多肽抗原特异性结合的证据

Specific binding of TCR molecule on human γδ T cells to Mycobacterium tuberculosis peptide antigen

利用GFP标签我们追踪到融合蛋白可与小肠粘膜发生特异性结合。

We may detect that the fusion protein is specially combined with intestinal mucosa by GFP .

结果表明，人亲环素33只与有poly（A）尾序列结构的mRNA，即poly（A）+RNA发生特异性结合。

The result showes that human cyclophilin 33 binds specifically with poly ( A ) tailing mRNA , namely poly ( A ) + RNA .

westernblot和细胞免疫荧光染色证实，该多克隆抗体能与MPS-1蛋白特异性结合。

The polyclonal antibody were found to specifically bind to MPS-1 protein in Western blot and immunofluorescent staining .

westernblot显示该融合蛋白相对分子质量为70×103，可与鼠抗人IL12p40p70单克隆抗体特异性结合。

Western blot showed the presence of a 70 × 10 3 band of the fusion protein , which specifically bond to mouse anti human IL 12 monoclonal antibody .

RNA结合蛋白（RNABindingProtein，RBPs）是一类与RNA分子特异性结合的蛋白质。

RNA binding proteins ( RNA binding protein , RBPs ) are a class of proteins which can directly binds to the RNA molecules .

应用蛋白质核酸紫外交联法检测多个细胞株，目的是明确是否存在可与HCV复制中间体3′末端特异性结合的细胞蛋白质。

UV cross-linking experiment was used to identify the cellular proteins which bind to the 3 ′ - end of HCV intermediate negative-strand RNA .