糖化酶

Southern印迹分析证明，糖化酶基因已整合进工程菌染色体。

The glucoamylase gene was integrated into the host chromosome as shown by Southern blotting .

CA外压管式超滤膜浓缩糖化酶

The application of Ca tubular external pressure ultrafiltration membrane to the concentration of Glucoamylase

然而高温淀粉酶的最适作用温度和pH与中温糖化酶均不相同，需要反复调整pH值和温度。

Because both enzymes have different optimum temperature and pH , the pH values and temperatures must be adjusted repeatedly .

TiO2膜吸附固定糖化酶特性的研究

Absorption properties of TiO_2 films for glucoamylase

TiO2膜固定的糖化酶使用8次后其剩余酶活仍能保持在72%。

The residual activity of glucoamylase immobilized on polypropylene TiO_2 film could maintain 72 % after being used eight times .

用PEG／（NH4）2SO4双水相体系萃取糖化酶

Extracting Glucoamylase by PEG / ( NH4_ ) _2SO_4 Aqueous Bi-Phase System

TiO2膜和PP.TiO2膜对糖化酶的吸附性能及稳定性能均较好，PP。

The acetyl cellulose TiO_2 film and polypropylene TiO_2 film showed better for glucoamylase absorption , and this immobilized glucoamylase also had higher stability .

结果表明，调节pH值法和添加表面活性剂法葡萄糖苷转移酶去除可达60%以上，且糖化酶酶活损失小于25%。

Surfactant-increasing method and pH-regulating method are the better methods as the removing rate of glucosyltransferase is more than 60 % , and the recovering rate of glucoamylase is less than 25 % .

葡萄糖异构酶高产菌株的诱变选育生淀粉糖化酶菌种黑曲霉S-1的激光选育

Mutagenesis and selection of raw starch glucoamylase-producing strains of aspergillus niger S-1 by laser irradiation

利用PEG/（NH4）2SO4双水相系统从粗酶液中提取糖化酶。

The glucoamylase was extracted and purified by PEG / ( NH4 ) 2SO4 double aqueous phase system .

采用聚乙烯醇（PVA）为载体共固定化酵母菌细胞和糖化酶制剂，并以木薯为原料进行酒精连续发酵工艺研究。

An alcoholic continuous fermentation using cassava as material , and by immobilization of glucoamylase preparation and yeasts using polyvinyl alcohol ( PVA ) gel as the carrier was studied .

PVP/（NH4）2SO4液&固萃取法提取糖化酶的研究

Studies on glucoamylase by using liquid-solid extraction system composed of PVP / ( nh_4 ) _2so_4

结果表明，发酵液糖化酶活力为6500U/ml，CMC酶活力为99mg葡萄糖/ml·h。

The results showed that the glucoamylase activity and CMC-enzyme activity of the mixed fermentation were 6500U / ml and 99 mg glucose / ml · h , which were 16 % and 266 % higher than that of A.

糖化酶用量125u/g原料，60℃糖化30～60min；

Use level of saccharifying enzyme as 125 u / g raw materials ; 30 ~ 60 min saccharification at 60 ℃;

利用产朊假丝酵母1087和热带假丝酵母1254，进行微生物发酵生产蛋白，探讨其对酒糟废液中COD去除率的影响，并进行复合酶和糖化酶在发酵中的作用进行了比较。

Using Candida utilis 1087 and Candida tropicalis 1254 , the microbial fermentation of protein was produced , and its lees liquid in the removal of COD was explore , also with the compare of enzymes and glucoamylase complex role in the fermentation .

通过在湘泉浓香型白酒的混蒸混烧工艺中添加TH-AADY和糖化酶，进行多季节连排生产试验。

TH-AADY and saccharifying enzyme were added during mixed-steaming and mixed-heating of Xiangquan Luzhou-flavor liquor for multiple-seasons consecutive production test .

黑曲霉突变株9-1-D糖化酶糖化淀粉条件研究

Conditions of starch saccharification catalyzed by glucoamylase produced by mutant of Aspergillus niger 9-1-D

在浓香型大曲酒丢糟中添加TH-AADY、糖化酶和纤维素酶酿造食醋，同时配合应用生香ADY。

TH-AADY , glucoamylase and cellulase were added in spent grains of Luzhou-flavor Daqu coupled with the use of aroma-producingADY to produce edible vinegar .

其次研究了α-淀粉酶、纤维素酶、木聚糖酶、糖化酶在薯蓣皂甙元得率提高中的作用，并首次利用RP-HPLC研究了这些外源酶对薯蓣皂素质量的影响。

In sucession , we studied the purpose of α - amylase , fibrin enzyme , xylan enzyme and γ - amylase on the increasing of extracting rate of diosgenin . And we first made use of RP-HPLC to indentify the quality of diosgenin .

工艺消除了木质素降解酶粗酶液及Novozymes漆酶直接抑制糖化酶作用，分别提高了糖得率19.9%和9.5%。

The process eliminated the effect that fermentation broth and Novozymes laccase directly inhibit glucoamylase , therefore , the rate of sugar yield increased by 19.9 % and 9.5 % , respectively .

对转化子进行摇瓶发酵研究，发酵终止时转化子GB0506的糖化酶活力比出发菌株F0410提高了17.5%。

Shake-flask fermentation under optimal conditions showed that glucoamylase secreted by the transformant GB0506 was17.5 % higher than parental strain F0410 at the end of fermentation .

黑曲霉糖化酶基因表达调控的研究&Ⅱ.A.nigerT21和3.795glaA基因5'调控区功能的体内分析

Research on the regulation of glucoamylase gene ( glaa ) expression in a.niger ──ⅱ . analysis of the function 0f 5 ' - regulatory region of A. niger T21 and 3.795 GLAA gene

用PEG/（NH4）2SO4双水相体系提取、纯化糖化酶，研究了影响体系分配系数、回收率和纯化因子的因素，从而确定了萃取糖化酶的最佳条件。

The saccharifying enzymes were extracted and purified by PEG / ( NH 4 ) 2 SO 4 double aqueous phases system . The factors affect-ing system distribution coefficients , recycling rate and purifying agents were studied and the optimal conditions of saccharifying enzymes extraction were then confirmed .

培菌阶段在豆腐坯表面积累了大量的蛋白酶、脂肪酶、α淀粉酶及糖化酶，培菌36h后菌体开始老化，酶活有所下降。

In the culture phase , a great deal of protease , lipase ,α _amylase and diastase were accumulated on the skin of beancurd . After 36 h , the thallus began to decay and the activity of all these enzymes began to decline .

糖化酶在制曲过程中活性呈逐渐增加趋势并在制曲后期达到最大值107.27U/g，在后发酵过程中活性逐渐降低，最后维持在45U/g左右。

Glucoamylase activity increased gradually to reach maximum 107.27U/g in the late koji stage , then decreased gradually in the post-fermentation process , and finally maintained at about 45U / g in the koji process .

从20种糖化酶制剂中优选出适于糖化玉米原料的2种酶制剂，用酵母菌株1031-R于40℃检验了适合玉米原料酒精发酵的条件。

Two enzyme preparations suitable for saccharification of raw corn grits were selected among about 20 kinds of saccharifying enzyme preparations . Suitable ethanol fermentation conditions of raw corn grits was examined using yeast strain 1031-R at 40 ℃ .

整合型转化子在以可溶性淀粉为碳源的培养基中分泌糖化酶活力达2.5u/ml，在非选择性培养基中连续转移10次，糖化酶分泌活力稳定不变。

The secreted glucoamylase activity of integrants in the medium with soluble starch as carbon source reached 2.5u/ml . After ten times successive transfers in nonselective medium the activity of secreted glucoamylase of integrant was approximately at its original level .

球形α-壳聚糖固定化糖化酶的比较研究

Comparative research on immobilization of glucoamylase with globe α - chitosan

纤维素酶产生菌和糖化酶产生菌液体混合发酵的研究

Liquid Mixed Fermentation by Cellulase Producing Strain and Glucoamylase Producing Strain

黑曲霉糖化酶基因在荧光假单胞菌中的表达

Expression of the Glucoamylase Gene of Aspergillus niger in Pseudomonas fluorescens