细胞分选

应用自动磁性细胞分选器分选人CD4~+CD（25）~+调节T细胞

Isolation human CD_4 ~ + CD_ ( 25 ) ~ + regulatory T cells with auto magnetic activated cell sorting

方法：用磁性细胞分选（MACS）和PCR技术分离、鉴定小鼠雄性胎肝Sca-1+细胞；

METHODS : The Sca-1 + cells from murine fetal liver were isolated with magnetic cell sorting ( MACS ) technique , the sex of which was identified by PCR .

应用磁激活细胞分选法从母血中分离胎儿有核红细胞做FISH行无创性产前诊断的研究

Non-invasive Prenatal Diagnosis From Maternal Blood : FISH of Fetal Nucleated Erythrocytes Isolated by Magnetic Activated Cell Sorting

OLPT淋巴细胞亚群分析及T细胞分选和体外培养初步研究

A Study of Assessment T Lymphocyte Subset of OLP Patient and Sorting and Culture T Lymphocyte in Vitro

本文应用流式细胞分选仪和电子显微镜研究了IL-3和羟基脲对人红白血病细胞株（K562细胞）凋亡的影响。结果显示：IL-3和羟基脲分别诱导K562细胞，不能引起细胞凋亡；

The effect of IL-3 and hydroxyurea on human erythroleukemia cell line ( K562 cells ) was demonstrated by using the electro-microscopy and flow cytometry .

采取ELISA检测腹腔液IL6水平，以流式细胞分选技术检测外周血T淋巴细胞亚群的变化。

The levels of IL 6 in peritoneal fluid were determined with ELISA , and the changes of T lymphocyte subpopulation in peripheral blood was also determined with flow cytometry technique .

本研究的目的是应用MidiMACS方法从急性髓系白血病（AML）患者骨髓细胞分选CD34+/CD123+细胞。

He aim of this study was to sort the CD34 ~ + / CD123 ~ + cells from the bone marrow cells of patients with acute myeloid leukemia ( AML ) by Midi MACS method .

方法：1、应用CD34+干细胞分选试剂盒和免疫磁珠法，经磁式细胞分选器（MACS）分离出人脐血CD34+干细胞；

Methods : ( 1 ) CD34 + hematopoietic stem cells were isolated from healthy human cord blood using CD34 + progenitor cell isolated kit by a high-gradient magnetic cells sorting system ( MACS ) .

利用磁吸附细胞分选法（MACS）分离大鼠脾脏CD4+T细胞，流式细胞术检测MACS分选前后CD4+T细胞比例，监测细胞分选效果。

Magnetic absorption cell sorting ( MACS ) method was used to isolate the CD4 + T lymphocytes from the spleen of the rats . Flow cytometry was used to monitor the efficiency of isolation .

目的评价免疫磁性细胞分选技术对乳腺癌患者的自体外周血干细胞（APBSC）的净化效果。

Objective To evaluate the effect of immunomagnetic technique in purging of peripheral blood progenitor cell ( PBPC ) of breast cancer patients .

标准化荧光激活细胞分选技术用于抗体定量是十分必要的。

A standardized fluorescence-activated cell sorting technique for antibody quantification is much needed .

结论荧光激活细胞分选技术是获得有效病毒滴度的包装细胞的有效方法。

Conclusion FACS is an efficient method for obtaining stable retroviral packaging cells .

结论：利用免疫荧光激活细胞分选术能分离出大量有活性的精原干细胞。

Conclusion : FACS can be used to isolate quantities of viable spermatogonial stem cells .

小鼠精原干细胞分选

Sorting of Spermatogonial Stem Cells in Mice

利用磁性细胞分选技术阴性选择法分离外周血嗜酸性粒细胞

Isolation of eosinophils from peripheral blood by the negative selection procedure of magnetic activated cell sorting

成人毛囊器官培养、干细胞分选及其增殖分化调控的研究

Study on the Whole-organ Culture of Adult Hair Follicle and Regulation of Selected Stem Cell 's Proliferation and Differentiation

主要观察指标：隐睾的形态学变化，精原干细胞分选结果。

MAIN OUTCOME MEASURES : The morphological changes of cryptorchidism , and the sorting results of spermatogonial stem cells .

目前国外采用较多的有荧光激活细胞分选法、磁活化细胞分选法、免疫磁珠分离法、密度梯度离心法。

Various methods of enrichment exit : fluorescent activated cell sorting , magnetic-activated cell sorting , immunomagnetic beads , density gradient .

目的用磁激活细胞分选技术富集并定量分析结直肠癌患者外周血循环中的癌细胞。

ObjectiveTo enrich and quantitatively analyze peripheral circulative carcinoma cells by magnetic activated cell sorting ( MACS ) in colorectal cancer patients .

目的：利用免疫磁性细胞分选系统分离纯化骨髓衍生肝干细胞亚群c-Kit+lin-。

AIM : To isolate and purify c-Kit + lin - cells from murine bone marrow by magnetic activated cell sorting ( MACS ) .

研究人员运用尖端的细胞分选技术，找出方法，证实人类和老鼠身上都存在卵巢干细胞。

Using a sophisticated cell-sorting technique , the researchers developed a way to identify ovarian stem cells that works for both mice and humans .

因此，广泛应用于免疫学检测、核酸纯化、细胞分选、药物载体和酶的固定化等生物医学领域。

Therefore , they are widely used in research of immunology detection , nucleic purification , cell sorting , drug carrier and biological enzyme immobilization .

目的探讨利用荧光激活细胞分选技术获得有效重组逆转录病毒包装细胞系的方法。

Objective To develop a method for acquisition of efficient and stable retroviral packaging cells on the basis of fluorescence-activated cell sorting ( FACS ) technique .

方法：采用磁性细胞分选技术的阴性选择法分离外周血嗜酸性粒细胞，并检测分离后嗜酸性粒细胞的纯度、存活率，计算其回收率。

Methods : Eosinophils of peripheral blood were isolated by the negative selection procedure of magnetic activated cell sorting and their purity and the ratio of viability and recovery were assessed .

然后通过免疫磁性细胞分选技术分离出播散的肿瘤细胞并进行培养，对培养成活的肿瘤细胞在鉴定来源的同时进行生物学活性检测，从细胞学角度判断入血肿瘤细胞形成转移灶的能力。

Secondly , we separated the tumor cells in blood samples by IMS and cultured it carefully , activities of the tumor cells has been tested to confirm their ability to form a successful metastasis .

利用免疫磁性细胞分选系统分选出的骨髓衍生肝干细胞亚群c-Kit+lin-细胞纯度和回收率分别为（77.98±2.34）%，75.40%，纯化前后细胞活力不受影响。

The cell purification and recovery rate of c-Kit + lin - cells were ( 77.98 ± 2.34 ) % , 75.40 % , respectively by MACS . The cell activity did not be affected before and after purification .

方法对2例病程分别为5年和7年，狼疮肾Ⅲ级和Ⅳ级，主要表现为持续性血小板减少、蛋白尿和浆膜炎的患儿进行CD34+细胞分选的自身干细胞移植。

Methods Two children with 5-year and 7-year duration of the lupus nephropathy at III and IV grade , respectively , mainly presented with persistent thrombocytopenia , proteinuria , and serositis received CD34 + autologous peripheral progenitor cells transplantation .

免疫磁珠细胞术分选脐血CD（34）~+细胞方法的改良

Improvement of sorting cord blood cd_ ( 34 ) ~ + cells by MACS

结论：合并使用G418及流式细胞仪分选是一种高效的筛选方法。

Conclusions It is a highly effective method to select the transfected cells using both G418 and FCM .

实验通过磁性纳米粒子与CD34单克隆抗体结合，构建磁性纳米粒子&生物分子复合物，制成细胞磁分选溶液。

Experiment through the magnetic nanoparticles with CD34 monoclonal antibody with magnetic nanoparticles to build - complex biological molecules , made of magnetic cell sorting solution .