细胞铺展

基于AFM形态学观察的细胞铺展机理的假想

Hypothesis of mechanism for cell spreading based on AFM morphological observation

在30min和60min两个时间点，Fn基质上细胞铺展分别降低了29%和26%；而在多聚赖氨酸基质上细胞铺展并没有变化。

Similarly , at 30-min and 60-min , the cell spreading on Fn but not on polylysine was inhibited by 29 % and 26 % respectively .

在经多聚赖氨酸衣被的表面临界脱离剪应力提高，细胞铺展生长。

It showed that the critical shear stress for cell detachment was relatively low on PHB , thus cells tend to aggregate and detach from surfaces .

抑制细胞迁移、铺展和局部粘附。

Inhibit cell migration , spread out and adhesiveness .

层粘连蛋白促进实体型瘤细胞粘着、铺展及~3H-TdR参人

Laminin stimulates the attachment , spread and ~ 3h-tdr incorporation of cancer cells

肝素图形样品还能促进内皮细胞粘附、铺展和增殖。

At the same time , the patterned heparin promoted cell adhesion , spreading and proliferation .

RGD修饰钛表面对人牙龈成纤维细胞初期黏附和铺展的影响

Influence of RGD-grafted titanium on the adhesion and spreading of human gingival fibroblasts

人肝癌细胞株HuH-7分泌铺展因子的研究

A Spreading Factor Secreted from Human Hepatoma Cell Line HuH-7 , in Serum-Free Medium

结果TCPHA和HA涂层材料表面黏附的细胞数、细胞铺展面积高于纯钛未涂层组，差异有显著性（P005），黏着斑的形成早于未涂层组；

Results TCP / HA and HA coatings exhibited that the attached cell number and cell spreading area were greater than those of CP titanium ( P 0 05 ), and the formation of focal adhesion plaque was earlier than that of uncoated substrate ( P 0 05 ) .

激光共聚焦显微镜观察发现，成骨细胞肌动蛋白纤维的铺展与钛片表面形貌有关。

Observed by LSCM , osteoblast spreading of actin fibers has relation to the surface topography .

目的探讨采用微弧氧化技术在钛表面注入钙、磷离子进行表面改性及其对成骨细胞早期附着和铺展的影响。

Objective To study the method for modifying titanium surface with calcium and phosphorus ions using micro-arc oxidation technique and observe osteoblast attachment to the modified surface .

血管内皮细胞&这一铺展于血管内腔、包绕着循环血的细胞，可合成和释放多种活性物质调节血液的凝血与抗凝平衡，在生物材料引起血栓形成的过程中起着重要的作用。

Vascular endothelial cells ( EC ), which envelope the circulating blood in a continuous monolayer , can synthesize and release large number of active mediators to regulate the hemostasis , and also play an important role in thrombosis caused by various kinds of biomaterials .

气道上皮的修复包括损伤边缘细胞变平、铺展、向损伤部位迁移和基细胞增殖分化成纤毛上皮细胞以及功能的恢复等环节。

It is reported that in the process of wound repair of airway epithelium , the cells around the wound initially get flat , then cells disseminate and migrate to the wound , and then basal cells proliferate and differentiate into ciliated epithelium with the recovery of the epithelium .

缺氧牵张12h细胞形态严重受损，微丝结构几乎完全破坏，细胞铺展面积变小，细胞完整性严重受损。

After three hour hypoxia and stretch , microfilament was damaged severely , microfilament was damaged completely , the spreading area of cell was shortened , and the whole cell was damaged severely .

细胞模板可以很好地调控细胞粘附的区域和细胞的铺展形态，利用该细胞模板获得了短期的内皮/平滑肌细胞联合培养体系。

The cell templates can be used to regulate cell adhesion and spreading on the patterns , and construct a short-term co-culture system of ECs / SMCs .

从人肝癌细胞株HuH－7的无血清培养基中分离出一种能促使细胞在体外铺展及生长的活性因子。

A heat-and trypsin-sensitive factor with cell-spreading and growth-promoting activity was isolated from human hepatoma cell line HuH-7 in a serum-free , hormone-supplemented medium .

实验结果表明：DPLA更有利于细胞的黏附，且高含量的丁二胺改性材料更有利于细胞的黏附与铺展。

The experimental results show that : DPLA more conducive to cell adhesion .