细胞周质

由于RGD水蛭素嵌合基因上游连接了金黄色葡萄球菌蛋白A（SPA）的信号肽序列，在IPTG诱导下两种嵌合分子都获得了分泌表达，表达产物主要集中在细胞周质空间。

Coli strain BL21 ( DE3 ), and gene expression was induced by IPTG . The target proteins were directed into the periplasmic space by the staphylococcal protein A ( SPA ) signal sequence preceding the RGD hirudin gene .

用超薄切片和原生质体负染的方法，在电镜下观察到洋葱鳞茎内表皮细胞周质微管的存在。

Cortical microtubules were observed in intraepidermal cells of onion bulb with thin section and negative staining of protoplasts .

虽然基因组序列分析预测可能的β-葡萄糖苷酶都位于细胞周质，但是多次酶活实验表明大部分的β-葡萄糖苷酶活力位于细胞表面。

Most of the β - glucosidase activity was located on the cell surface of C. hutchinsonii from the enzyme assay , although the genomic analysis showed that all the candidate β - glucosidases were predicted to be located in the periplasmic space .

而后锰蛋白转移到细胞外周质，开始对锰原子进行捕获。

The manganese protein is then transported to the periplasm having first trapped its manganese .